Disposable electrochemical sensor: Highly sensitive determination of nitrofurazone antibiotic in environmental samples and pharmaceutical formulations.

The study presents the successful development of a new electrochemical sensor with low cost and disposability for application in nitrofurazone detection in environmental and pharmaceutical samples. The sensors were fabricated using materials obtained from local storage and conductive carbon ink. The modification of the screen-printed electrodes with the hybrid nanomaterial based on silver nanoparticles, carbon quantum dots, and carbon nanotubes showed synergistic contributions in the nitrofurazone electrooxidation, as observed in the wide linear range (0.008 at 15.051 µM), with a sensitivity of 0.650 µA/µM. The limit of detection obtained was 4.6 nM. Differential pulse voltammetry, cyclic voltammetry, X-ray photoelectron spectroscopy, X-ray diffraction analysis, and high-resolution transmission electron microscopy were used to evaluate the electrochemical and structural characteristics. Studies of possible interferences were considered with nitrofurazone in the presence of the ions and organic molecules. The results were satisfactory, with a variation of 93.3% ± 4.39% at 100% ± 2.40%. The low volume used in the analyses (50 µL), disposability, high sensibility, selectivity, and low limit of detection are advantages that make the proposed sensor an electrochemical tool of high viability for the NFZ detection in environmental matrices and pharmaceutical formulations.

Interaction between nitroheterocyclic compounds with beta-cyclodextrins: phase solubility and HPLC studies.

Chagas disease is a serious health problem for Latin America. Nitrofurazone (NF) and Hidroxymethylnitrofurazone (NFOH) are active against Trypanosoma cruzi. The effect of beta-cyclodextrin (beta-CD) and dimethyl-beta-cyclodextrin (DM-beta-CD) complexation on the UV absorption and retention time of nitrofurazone (NF) and its hydroxymethylated analog (NFOH) were studied in solution. The retention behavior was analyzed on a reversed phase C18 column and the mobile phase used was acetonitrile-water (20/80 v/v), in which cyclodextrins (beta-CD or DM-beta-CD) were incorporated as a mobile phase additive. The decrease in the retention times of NF (or NFOH) with increasing concentration of HP-beta-CD enables the determination of the complex stability constants by HPLC. A phase-solubility study was performed, according to the method reported by Higuchi and Connors, to evaluate the changes of NF/NFOH in the complexation state, and the diagrams obtained suggested that it forms complexes with a stoichiometry of 1:1. This is an important study for the characterization of potential formulations to be used as therapeutic options for the treatment of Chagas disease.

Atividade antibacteriana de medicamentos endodônticos sobre bactérias anaeróbias estritas / Evaluation of the antibacterial activities of some endodontic medicaments against anaerobic bacteria

Avaliou-se a atividade antibacteriana dos medicamentos paramonoclorofenol (PMC) canforado; PMC associado ao Furacin e PMC aquoso a 2 por cento e das pastas de hidróxido de cálcio em água destilada ou em PMC aquoso a 2 por cento sobre principalmente bactérias anaeróbias estritas, os principais patógenos endodônticos. Os resultados demonstraram maior eficácia antibacteriana do PMC canforado, do PMC/Furacin e de pasta de hidróxido de cálcio/PMC a 2 por cento. O PMC aquoso a 2 por cento apresentou baixa atividade antibacteriana, enquanto que a pasta de hidróxido de cálcio em água destilada evidenciou ausência completa de efeito inibitório contra todas as cepas bacterianas testadas

Resposta pulpar frente à técnicas de tratamento com misturas de PMCC + furacin, óxido de zinco e eugenol, após pulpotomia em dentes de cães / Pulp response to treatment techniques using compounds of CMPC + furacin and zinc oxide-eugenol, after pulpotomy in teeth of dogs

Avaliou-se histopatologicamente a resposta pulpar de dentes de cães submetidos a pulpotomia e tratamento do remanescente pulpar com misturas de paramonoclorofenol canforado (3,5 : 6,5) e (2,5 : 7,5) mais furacin e ou óxido de zinco e eugneol em períodos de 7, 45 e 90 dias. Concluiu-se que as misturas de paramonoclorofenol canfordo (3,5 : 6,5) e (2,5 : 7,5) mais furacin mostraram-se irritantes ao tecido conjuntivo pulpar, levando à morte pulpar em 86,75 por cento e 60,98 por cento, respectivamente. O óxido de zinco e eugenol mostrou melhor comportamento, observando-se vitalidade pulpar (89,42 por cento), irritação persistente e decrescente no decorrer dos períodos. Em todos os casos observou-se manutenção parcial ou total das vitalidades nas extensões conjuntivas do delta apical menos expressiva no Grupo PMCC (3,5 : 6,5) - furacin

Ensayo de estabilidad para nitrofurazona crema / Stability assay for nitrofurazone crea

Se analiza un estudio de la estabilidad del preparado. Las muestras se colocan a varias temperaturas posteriormente se analizaron por espectofotometría en la zona ultravioleta. Además se realizan ensayos cromatográficos utilizando como solvente una mezcla de benceno acetona 3-2. Se realiza un ensayo comparativo de las muestras colocadas a diferentes temperaturas. Se le da fecha de vencimiento de acuerdo con los resultados obtenidos

Ensayo de estabilidad para nitrofurazona crema / Stability assay for nitrofurazone cream

Se analiza un estudio de la estabilidad del preparado. Las muestras se colocan a varias temperaturas posteriormente se analizaron por espectofotometría en la zona ultravioleta. Además se realizan ensayos cromatográficos utilizando como solvente una mezcla de benceno acetona 3-2. Se realiza un ensayo comparativo de las muestras colocadas a diferentes temperaturas. Se le da fecha de vencimiento de acuerdo con los resultados obtenidos