RESUMEN
Oocyte composition can directly influence offspring fitness, particularly in oviparous species such as most insects, where it is the primary form of parental investment. Oocyte production is also energetically costly, dependent on female condition and responsive to external cues. Here, we investigated whether mating influences mature oocyte composition in Drosophila melanogaster using a quantitative proteomic approach. Our analyses robustly identified 4,485 oocyte proteins and revealed that stage-14 oocytes from mated females differed significantly in protein composition relative to oocytes from unmated females. Proteins forming a highly interconnected network enriched for translational machinery and transmembrane proteins were increased in oocytes from mated females, including calcium binding and transport proteins. This mating-induced modulation of oocyte maturation was also significantly associated with proteome changes that are known to be triggered by egg activation. We propose that these compositional changes are likely to have fitness consequences and adaptive implications given the importance of oocyte protein composition, rather than active gene expression, to the maternal-to-zygotic transition and early embryogenesis.
Asunto(s)
Proteínas de Drosophila/genética , Drosophila melanogaster/genética , Oocitos/metabolismo , Oogénesis/genética , Proteoma/genética , Cigoto/metabolismo , Animales , Proteínas de Unión al Calcio/clasificación , Proteínas de Unión al Calcio/genética , Proteínas de Unión al Calcio/metabolismo , Proteínas Portadoras/clasificación , Proteínas Portadoras/genética , Proteínas Portadoras/metabolismo , Copulación/fisiología , Proteínas de Drosophila/clasificación , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/metabolismo , Femenino , Fertilización/genética , Perfilación de la Expresión Génica , Regulación de la Expresión Génica , Ontología de Genes , Aptitud Genética , Masculino , Proteínas de la Membrana/clasificación , Proteínas de la Membrana/genética , Proteínas de la Membrana/metabolismo , Anotación de Secuencia Molecular , Oocitos/citología , Oocitos/crecimiento & desarrollo , Biosíntesis de Proteínas , Proteoma/clasificación , Proteoma/metabolismo , Espermatozoides/citología , Espermatozoides/fisiología , Cigoto/citología , Cigoto/crecimiento & desarrolloRESUMEN
BACKGROUND: Staphylococcus aureus cell wall anchored Serine Aspartate repeat containing protein D (SdrD) is a member of the microbial surface component recognising adhesive matrix molecules (MSCRAMMs). It is involved in the bacterial adhesion and virulence. However the extent of genetic variation in S. aureus sdrD gene within isolates from healthy carriers are not known. The aim of this study was to evaluate allelic variation of the sdrD gene among S. aureus from healthy nasal carriers. RESULTS: The sdrD A region from 48 S. aureus isolates from healthy carriers were analysed and classified into seven variants. Variations in the sdrD A region were concentrated in the N2 and N3 subdomains. Sequence analysis of the entire sdrD gene of representative isolates revealed variations in the SD repeat and the EF motifs of the B repeat. In silico structural modelling indicates that there are no differences in the SdrD structure of the 7 variants. Variable amino acid residues mapped onto the 3D structure revealed that the variations are surface located, exist within the groove between the N2-N3 subdomains and distributed mainly on the N3 subdomain. Comparison of adhesion to keratinocytes in an in vitro cell adhesion assay, using NCTC 8325-4∆sdrD strains expressing the various sdrD gene variants, indicated a significant difference between only two complements while others showed no major difference in their adhesion. CONCLUSIONS: This study provides evidence of sequence variations across the different domains of SdrD from S. aureus isolated from healthy nasal carriers. Proper understanding of these variations is necessary in the study of S. aureus pathogenesis.
Asunto(s)
Proteínas Bacterianas/genética , Proteínas de Unión al Calcio/genética , Variación Genética , Nariz/microbiología , Staphylococcus aureus/genética , Secuencia de Aminoácidos , Adhesión Bacteriana , Proteínas Bacterianas/clasificación , Proteínas Bacterianas/aislamiento & purificación , Proteínas de Unión al Calcio/clasificación , Proteínas de Unión al Calcio/aislamiento & purificación , Línea Celular , Humanos , Queratinocitos/microbiología , Modelos Moleculares , Tipificación de Secuencias Multilocus , Filogenia , Conformación Proteica , Dominios Proteicos , Infecciones Estafilocócicas/microbiología , Staphylococcus aureus/aislamiento & purificación , Virulencia/genéticaRESUMEN
Calcium ions are involved in the regulation of diverse cellular processes. Fourteen genes encoding calcium binding proteins have been identified in Dictyostelium. CBP7, one of the 14 CBPs, is composed of 169 amino acids and contains four EF-hand motifs. Here, we investigated the roles of CBP7 in the development and cell migration of Dictyostelium cells and found that high levels of CBP7 exerted a negative effect on cells aggregation during development, possibly by inhibiting chemoattractant-directed cell migration. While cells lacking CBP7 exhibited normal development and chemotaxis similar that of wild-type cells, CBP7 overexpressing cells completely lost their chemotactic abilities to move toward increasing cAMP concentrations. This resulted in inhibition of cellular aggregation, a process required for forming multicellular organisms during development. Low levels of cytosolic free calcium were observed in CBP7 overexpressing cells, which was likely the underlying cause of their lack of chemotaxis. Our results demonstrate that CBP7 plays an important role in cell spreading and cell-substrate adhesion. cbp7 null cells showed decreased cell size and cell-substrate adhesion. The present study contributes to further understanding the role of calcium signaling in regulation of cell migration and development.
Asunto(s)
Proteínas de Unión al Calcio/metabolismo , Quimiotaxis , Dictyostelium/metabolismo , Transducción de Señal , Calcio/metabolismo , Proteínas de Unión al Calcio/clasificación , Proteínas de Unión al Calcio/genética , Factores Quimiotácticos/genética , Factores Quimiotácticos/metabolismo , AMP Cíclico/metabolismo , Dictyostelium/citología , Dictyostelium/genética , Movimiento , FilogeniaRESUMEN
Numerous oil bodies of 0.2-2 µm occupied approximately 80% of intracellular space in mature pine (Pinus massoniana) megagametophytes. They were stably isolated and found to comprise mostly triacylglycerols as examined by thin layer chromatography analysis and confirmed by both Nile red and BODIPY stainings. Fatty acids released from the triacylglycerols of pine oil bodies were mainly unsaturated, including linoleic acid (60%), adrenic acid (12.3%) and vaccenic acid (9.7%). Proteins extracted from pine oil bodies were subjected to immunological cross-recognition, and the results showed that three proteins of 28, 16 and 14 kDa were detected by antibodies against sesame seed caleosin, sesame oleosin-L and lily pollen oleosin-P, respectively. Complete cDNA fragments encoding these three pine oil-body proteins, tentatively named caleosin, oleosin-L and oleosin-G, were obtained by PCR cloning and further confirmed by mass spectrometric analysis. Consistently, phylogenetic tree analyses showed that pine caleosin was closely-related to the caleosin of cycad megagametophyte among known caleosin sequences. While pine oleosin-L was found clustered with seed oleosin isoforms of angiosperm species, oleosin-G was distinctively grouped with the oleosin-P of lily pollen. The oleosin-G identified in pine megagametophytes seems to represent a new class of seed oleosin isoform evolutionarily close to the pollen oleosin-P.
Asunto(s)
Proteínas de Unión al Calcio/genética , Gotas Lipídicas/metabolismo , Proteínas de Plantas/genética , Proteínas de Unión al Calcio/química , Proteínas de Unión al Calcio/clasificación , Filogenia , Pinus/genética , Pinus/metabolismo , Proteínas de Plantas/química , Proteínas de Plantas/clasificación , Polen/genética , Polen/metabolismo , Isoformas de Proteínas/química , Isoformas de Proteínas/clasificación , Isoformas de Proteínas/genéticaRESUMEN
Caleosin is a common lipid-droplet surface protein, which has the ability to bind calcium. Arabidopsis (Arabidopsis thaliana) is considered a model organism in plant researches. Although there are growing researches about caleosin in the past few years, a systemic analysis of caleosins in Arabidopsis is still scarce. In this study, a comprehensive investigation of caleosins in Arabidopsis was performed by bioinformatics methods. Firstly, eight caleosins in Arabidopsis are divided into two types, L-caleosin and H-caleosin, according to their molecular weights, and these two types of caleosin have many differences in characteristics. Secondly, phylogenetic tree result indicates that L-caleosin may evolve from H-caleosin. Thirdly, duplication pattern analysis shows that segmental and tandem duplication are main reasons for Arabidopsis caleosin expansion with the equal part. Fourthly, the expression profiles of caleosins are also investigated in silico in different organs and under various stresses and hormones. In addition, based on promoter analysis, caleosin may be involved in calcium signal transduction and lipid accumulation. Thus, the classification and expression analysis of caleosin genes in Arabidopsis provide facilities to the research of phylogeny and functions in this gene family.
Asunto(s)
Proteínas de Arabidopsis/genética , Arabidopsis/genética , Proteínas de Unión al Calcio/genética , Genes de Plantas , Proteínas de Plantas/genética , Secuencia de Aminoácidos , Arabidopsis/metabolismo , Proteínas de Arabidopsis/clasificación , Proteínas de Arabidopsis/metabolismo , Señalización del Calcio/genética , Proteínas de Unión al Calcio/clasificación , Proteínas de Unión al Calcio/metabolismo , Mapeo Cromosómico , Evolución Molecular , Duplicación de Gen , Regulación de la Expresión Génica de las Plantas , Genómica , Metabolismo de los Lípidos/genética , Datos de Secuencia Molecular , Familia de Multigenes , Filogenia , Aceites de Plantas/metabolismo , Proteínas de Plantas/clasificación , Proteínas de Plantas/metabolismo , Regiones Promotoras Genéticas , Homología de Secuencia de AminoácidoRESUMEN
Nesfatin-1 is an eighty two amino acid, naturally occurring multifunctional protein encoded in the precursor nucleobindin-2 (NUCB2). A comparison of sequences indicates that NUCB2 is present in a number of animals, from hydra to humans. The 30 amino acid mid-segment of nesfatin-1 is considered to be the bioactive core of the protein, and this region displays the highest identity among nesfatin-1 sequences reported thus far. Similar to the sequence relationships observed, the tissue-specific expression and biological actions of nesfatin-1 also appear to be highly conserved across species. For example, brain is a major tissue abundantly expressing nesfatin- 1 in several species. It has been shown that various key regions of the rat, mouse and goldfish brain, which are involved in the regulation of feeding and metabolism express nesfatin-1. Exogenous administration of nesfatin-1 results in a decrease in the food intake of rats, mice and goldfish. In addition, nesfatin-1 has been shown to regulate a number of other physiological processes including hormone secretion from the pancreatic islets and pituitary gland, stress and behavior. While nesfatin-1 research still remains an emerging area in physiology, the literature available thus far clearly shows that nesfatin-1 is an important regulator of homeostasis in animals.
Asunto(s)
Proteínas de Unión al Calcio/clasificación , Proteínas de Unión al ADN/clasificación , Proteínas del Tejido Nervioso/clasificación , Filogenia , Animales , Proteínas de Unión al Calcio/genética , Proteínas de Unión al Calcio/fisiología , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/fisiología , Humanos , Proteínas del Tejido Nervioso/genética , Proteínas del Tejido Nervioso/fisiología , NucleobindinasRESUMEN
Calcium-binding photoproteins have been discovered in a variety of luminous marine organisms [1]. Recent interest in photoproteins from the phylum Ctenophora has stemmed from cloning and expression of several photoproteins from this group [2-5]. Additional characterization has revealed unique biochemical properties found only in ctenophore photoproteins, such as inactivation by light. Here we report the cloning, expression, and characterization of the photoprotein responsible for luminescence in the deep-sea ctenophore Bathocyroe fosteri. This animal was of particular interest due to the unique broad color spectrum observed in live specimens [6]. Full-length sequences were identified by BLAST searches of known photoprotein sequences against Bathocyroe transcripts obtained from 454 sequencing. Recombinantly expressed Bathocyroe photoprotein (BfosPP) displayed an optimal coelenterazine-loading pH of 8.5, and produced calcium-triggered luminescence with peak wavelengths closely matching the 493 nm peak observed in the spectrum of live B. fosteri specimens. Luminescence from recombinant BfosPP was inactivated most efficiently by UV and blue light. Primary structure alignment of BfosPP with other characterized photoproteins showed very strong sequence similarity to other ctenophore photoproteins and conservation of EF-hand motifs. Both alignment and structural prediction data provide more insight into the formation of the coelenterazine-binding domain and the probable mechanism of photoinactivation.
Asunto(s)
Proteínas de Unión al Calcio/química , Proteínas de Unión al Calcio/efectos de la radiación , Ctenóforos/metabolismo , Luminiscencia , Proteínas Luminiscentes/química , Proteínas Luminiscentes/efectos de la radiación , Secuencia de Aminoácidos , Animales , Calcio/metabolismo , Proteínas de Unión al Calcio/clasificación , Proteínas de Unión al Calcio/genética , Clonación Molecular , Imidazoles/química , Sustancias Luminiscentes/química , Proteínas Luminiscentes/clasificación , Proteínas Luminiscentes/genética , Datos de Secuencia Molecular , Filogenia , Conformación Proteica , Pirazinas/químicaRESUMEN
Centrins are a ubiquitous family of small Ca(2+)-binding proteins found at basal bodies that are placed into two groups based on sequence similarity to the human centrins 2 and 3. Analyses of basal body composition in different species suggest that they contain a centrin isoform from each group. We used the ciliate protist Tetrahymena thermophila to gain a better understanding of the functions of the two centrin groups and to determine their potential redundancy. We have previously shown that the Tetrahymena centrin 1 (Cen1), a human centrin 2 homologue, is required for proper basal body function. In this paper, we show that the Tetrahymena centrin 2 (Cen2), a human centrin 3 homologue, has functions similar to Cen1 in basal body orientation, maintenance, and separation. The two are, however, not redundant. A further examination of human centrin 3 homologues shows that they function in a manner distinct from human centrin 2 homologues. Our data suggest that basal bodies require a centrin from both groups in order to function correctly.
Asunto(s)
Proteínas de Unión al Calcio/metabolismo , Centriolos/metabolismo , Proteínas Protozoarias/metabolismo , Tetrahymena thermophila/metabolismo , Secuencia de Aminoácidos , Western Blotting , Proteínas de Unión al Calcio/clasificación , Proteínas de Unión al Calcio/genética , Proteínas de Ciclo Celular/clasificación , Proteínas de Ciclo Celular/genética , Proteínas de Ciclo Celular/metabolismo , Centriolos/ultraestructura , Expresión Génica , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Humanos , Microscopía Electrónica , Microscopía Fluorescente , Datos de Secuencia Molecular , Mutación , Filogenia , Isoformas de Proteínas/clasificación , Isoformas de Proteínas/genética , Isoformas de Proteínas/metabolismo , Proteínas Protozoarias/clasificación , Proteínas Protozoarias/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Homología de Secuencia de Aminoácido , Tetrahymena thermophila/genética , Tetrahymena thermophila/ultraestructuraRESUMEN
In plants, epoxygenated fatty acids (EFAs) are constituents of oil seeds as well as defence molecules and components of biopolymers (cutin, suberin). While the pleiotropic biological activities of mammalian EFAs have been well documented, there is a paucity of information on the physiological relevance of plant EFAs and their biosynthesis. Potential candidates for EFA formation are caleosin-type peroxygenases which catalyze the epoxidation of unsaturated fatty acids in the presence of hydroperoxides as co-oxidants. However, the caleosins characterized so far, which are mostly localized in seeds, are poor epoxidases. In sharp contrast, quantitative RT-PCR analysis revealed that PXG4, a class II caleosin gene, is expressed in roots, stems, leaves and flowers of Arabidopsis. Expressed in yeast, PXG4 encodes a calcium-dependent membrane-associated hemoprotein able to catalyze typical peroxygenase reactions. Moreover, we show here that purified recombinant PXG4 is an efficient fatty acid epoxygenase, catalyzing the oxidation of cis double bonds of unsaturated fatty acids. Physiological linoleic and linolenic acids proved to be the preferred substrates for PXG4; they are oxidized into the different positional isomers of the monoepoxides and into diepoxides. An important regioselectivity was observed; the C-12,13 double bond of these unsaturated fatty acids being the least favored unsaturation epoxidized by PXG4, linolenic acid preferentially yielded the 9,10-15,16-diepoxide. Remarkably, PXG4 catalyzes exclusively the formation of (R),(S)-epoxide enantiomers, which is the absolute stereochemistry of the epoxides found in planta. These findings pave the way for the study of the functional role of EFAs and caleosins in plants.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Proteínas de Unión al Calcio/metabolismo , Ácidos Grasos Insaturados/metabolismo , Oxigenasas de Función Mixta/metabolismo , Oxidorreductasas/metabolismo , Arabidopsis/genética , Proteínas de Arabidopsis/genética , Biocatálisis , Western Blotting , Proteínas de Unión al Calcio/clasificación , Proteínas de Unión al Calcio/genética , Ácidos Grasos Insaturados/química , Regulación Enzimológica de la Expresión Génica , Regulación de la Expresión Génica de las Plantas , Ácidos Linoleicos/química , Ácidos Linoleicos/metabolismo , Ácidos Linolénicos/química , Ácidos Linolénicos/metabolismo , Oxigenasas de Función Mixta/clasificación , Oxigenasas de Función Mixta/genética , Oxidación-Reducción , Oxidorreductasas/genética , Oxígeno/metabolismo , Filogenia , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Estereoisomerismo , Especificidad por SustratoRESUMEN
Trophic endosymbiosis between anthozoans and photosynthetic dinoflagellates forms the key foundation of reef ecosystems. Dysfunction and collapse of symbiosis lead to bleaching (symbiont expulsion), which is responsible for the severe worldwide decline of coral reefs. Molecular signals are central to the stability of this partnership and are therefore closely related to coral health. To decipher inter-partner signaling, we developed genomic resources (cDNA library and microarrays) from the symbiotic sea anemone Anemonia viridis. Here we describe differential expression between symbiotic (also called zooxanthellate anemones) or aposymbiotic (also called bleached) A. viridis specimens, using microarray hybridizations and qPCR experiments. We mapped, for the first time, transcript abundance separately in the epidermal cell layer and the gastrodermal cells that host photosynthetic symbionts. Transcriptomic profiles showed large inter-individual variability, indicating that aposymbiosis could be induced by different pathways. We defined a restricted subset of 39 common genes that are characteristic of the symbiotic or aposymbiotic states. We demonstrated that transcription of many genes belonging to this set is specifically enhanced in the symbiotic cells (gastroderm). A model is proposed where the aposymbiotic and therefore heterotrophic state triggers vesicular trafficking, whereas the symbiotic and therefore autotrophic state favors metabolic exchanges between host and symbiont. Several genetic pathways were investigated in more detail: i) a key vitamin K-dependant process involved in the dinoflagellate-cnidarian recognition; ii) two cnidarian tissue-specific carbonic anhydrases involved in the carbon transfer from the environment to the intracellular symbionts; iii) host collagen synthesis, mostly supported by the symbiotic tissue. Further, we identified specific gene duplications and showed that the cnidarian-specific isoform was also up-regulated both in the symbiotic state and in the gastroderm. Our results thus offer new insight into the inter-partner signaling required for the physiological mechanisms of the symbiosis that is crucial for coral health.
Asunto(s)
Dinoflagelados/genética , Anémonas de Mar/genética , Simbiosis/genética , Transcriptoma , Secuencia de Aminoácidos , Animales , Proteínas de Unión al Calcio/clasificación , Proteínas de Unión al Calcio/genética , Moléculas de Adhesión Celular/clasificación , Moléculas de Adhesión Celular/genética , Dinoflagelados/fisiología , Francia , Perfilación de la Expresión Génica , Geografía , Interacciones Huésped-Parásitos/genética , Calor , Mar Mediterráneo , Proteínas de la Membrana/clasificación , Proteínas de la Membrana/genética , Datos de Secuencia Molecular , Análisis de Secuencia por Matrices de Oligonucleótidos , Filogenia , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Anémonas de Mar/parasitología , Homología de Secuencia de Aminoácido , Factores de TiempoRESUMEN
During adaptation and developmental processes cells respond through nonlinear calcium-decoding signaling cascades, the principal components of which have been identified. However, the molecular mechanisms generating specificity of cellular responses remain poorly understood. Calcineurin B-like (CBL) proteins contribute to decoding calcium signals by specifically interacting with a group of CBL-interacting protein kinases (CIPKs). Here, we report the subcellular localization of all 10 CBL proteins from Arabidopsis and provide a cellular localization matrix of a plant calcium signaling network. Our findings suggest that individual CBL proteins decode calcium signals not only at the plasma membrane and the tonoplast, but also in the cytoplasm and nucleus. We found that distinct targeting signals located in the N-terminal domain of CBL proteins determine the spatially discrete localization of CBL/CIPK complexes by COPII-independent targeting pathways. Our findings establish the CBL/CIPK signaling network as a calcium decoding system that enables the simultaneous specific information processing of calcium signals emanating from different intra- and extracellular stores, and thereby provides a mechanism underlying the specificity of cellular responses.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Proteínas de Unión al Calcio/metabolismo , Calcio/metabolismo , Proteínas Serina-Treonina Quinasas/metabolismo , Transducción de Señal , Arabidopsis/genética , Arabidopsis/metabolismo , Proteínas de Arabidopsis/clasificación , Proteínas de Arabidopsis/genética , Western Blotting , Proteínas de Unión al Calcio/clasificación , Proteínas de Unión al Calcio/genética , Regulación de la Expresión Génica de las Plantas , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Microscopía Confocal , Filogenia , Unión Proteica , Proteínas Serina-Treonina Quinasas/genética , Protoplastos/metabolismo , Nicotiana/genética , Nicotiana/metabolismo , Vacuolas/metabolismoRESUMEN
Regulators of calcineurin (RCANs) in fungi and mammals have been shown to stimulate and inhibit calcineurin signaling in vivo through direct interactions with the catalytic subunit of the phosphatase. The dual effects of RCANs on calcineurin were examined by performing structure-function analyses on yeast Rcn1 and human RCAN1 (a.k.a. DSCR1, MCIP1, and calcipressin 1) proteins expressed at a variety of different levels in yeast. At high levels of expression, the inhibitory effects required a degenerate PxIxIT-like motif and a novel LxxP motif, which may be related to calcineurin-binding motifs in human NFAT proteins. The conserved glycogen synthase kinase 3 (GSK-3) phosphorylation site was not required for inhibition, suggesting that RCANs can simply compete with other substrates for docking onto calcineurin. In addition to these docking motifs, two other highly conserved motifs plus the GSK-3 phosphorylation site in RCANs, along with the E3 ubiquitin ligase SCF(Cdc4), were required for stimulation of calcineurin signaling in yeast. These findings suggest that RCANs may function primarily as chaperones for calcineurin biosynthesis or recycling, requiring binding, phosphorylation, ubiquitylation, and proteasomal degradation for their stimulatory effect. Finally, another highly divergent yeast RCAN, termed Rcn2 (YOR220w), was identified through a functional genetic screen. Rcn2 lacks all stimulatory motifs, though its expression was still strongly induced by calcineurin signaling through Crz1 and it competed with other endogenous substrates when overexpressed, similar to canonical RCANs. These findings suggest a primary role for canonical RCANs in facilitating calcineurin signaling, but canonical RCANs may secondarily inhibit calcineurin signaling by interfering with substrate interactions and enzymatic activity.
Asunto(s)
Calcineurina/metabolismo , Proteínas de Unión al Calcio/metabolismo , Proteínas Portadoras/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Saccharomyces cerevisiae/metabolismo , Transducción de Señal/fisiología , Proteínas Adaptadoras Transductoras de Señales , Secuencia de Aminoácidos , Animales , Proteínas de Unión al Calcio/clasificación , Proteínas de Unión al Calcio/genética , Proteínas Portadoras/clasificación , Proteínas Portadoras/genética , Proteínas de Ciclo Celular/genética , Proteínas de Ciclo Celular/metabolismo , Proteínas de Unión al ADN , Evolución Molecular , Proteínas F-Box/genética , Proteínas F-Box/metabolismo , Humanos , Péptidos y Proteínas de Señalización Intracelular/clasificación , Péptidos y Proteínas de Señalización Intracelular/genética , Péptidos y Proteínas de Señalización Intracelular/metabolismo , Datos de Secuencia Molecular , Proteínas Musculares/clasificación , Proteínas Musculares/genética , Proteínas Musculares/metabolismo , Factores de Transcripción NFATC/genética , Factores de Transcripción NFATC/metabolismo , Filogenia , Estructura Terciaria de Proteína , Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/clasificación , Proteínas de Saccharomyces cerevisiae/genética , Ubiquitina-Proteína Ligasas/genética , Ubiquitina-Proteína Ligasas/metabolismoRESUMEN
Although many aspects of the physiological and pathophysiological mechanisms remain unknown, recent advances in our knowledge suggest that the CREC proteins are promising disease biomarkers or targets for therapeutic intervention in a variety of diseases. The CREC family of low affinity, Ca2+-binding, multiple EF-hand proteins are encoded by five genes, RCN1, RCN2, RCN3, SDF4, and CALU, resulting in reticulocalbin, ER Ca2+-binding protein of 55 kDa (ERC-55), reticulocalbin-3, Ca2+-binding protein of 45 kDa (Cab45), and calumenin. Alternative splicing increases the number of gene products. The proteins are localized in the cytosol, in various parts of the secretory pathway, secreted to the extracellular space or localized on the cell surface. The emerging functions appear to be highly diverse. The proteins interact with several different ligands. Rather well-described functions are attached to calumenin with the inhibition of several proteins in the endoplasmic or sarcoplasmic reticulum membrane, the vitamin K(1) 2,3-epoxide reductase, the gamma-carboxylase, the ryanodine receptor, and the Ca2+-transporting ATPase. Other functions concern participation in the secretory process, chaperone activity, signal transduction as well as participation in a large variety of disease processes.
Asunto(s)
Proteínas de Unión al Calcio/clasificación , Proteínas de Unión al Calcio/metabolismo , Enfermedad , Animales , Proteínas de Unión al Calcio/química , Proteínas de Unión al Calcio/genética , Humanos , Transporte de Proteínas , Transcripción Genética/genéticaRESUMEN
The NOD-like receptor (NLR) family of proteins is involved in the regulation of innate immune responses and cell death pathways. Recent findings show that the NLR family member NLRC4 (also known as IPAF) has important roles in innate immune responses to Gram-negative bacteria. Macrophages infected with Legionella pneumophila, Salmonella typhimurium, Shigella flexneri, or Pseudomonas aeruginosa activate caspase-1 in an NLRC4-dependent manner leading to macrophage cell death and the release of proinflammatory cytokines. This review will discuss these findings as well as the role of bacterial type III and type IV secretion systems and flagellin in NLRC4-mediated caspase-1 activation.
Asunto(s)
Proteínas Adaptadoras de Señalización CARD/metabolismo , Proteínas de Unión al Calcio/metabolismo , Animales , Proteínas Adaptadoras de Señalización CARD/clasificación , Proteínas de Unión al Calcio/clasificación , Caspasa 1/metabolismo , Activación Enzimática , Humanos , Inflamación/inmunología , Inflamación/microbiología , Proteína Inhibidora de la Apoptosis Neuronal/metabolismoRESUMEN
Calcium serves as a critical messenger in many adaptation and developmental processes. Cellular calcium signals are detected and transmitted by sensor molecules such as calcium-binding proteins. In plants, the calcineurin B-like protein (CBL) family represents a unique group of calcium sensors and plays a key role in decoding calcium transients by specifically interacting with and regulating a family of protein kinases (CIPKs). Several CBL proteins appear to be targeted to the plasma membrane by means of dual lipid modification by myristoylation and S-acylation. In addition, CBL/CIPK complexes have been identified in other cellular localizations, suggesting that this network may confer spatial specificity in Ca2+ signaling. Molecular genetics analyses of loss-of function mutants have implicated several CBL proteins and CIPKs as important components of abiotic stress responses, hormone reactions and ion transport processes. The occurrence of CBL and CIPK proteins appears not to be restricted to the plant kingdom raising the question about the function of these Ca2+ decoding components in non-plant species.
Asunto(s)
Calcineurina/metabolismo , Proteínas de Unión al Calcio/metabolismo , Calcio/metabolismo , Proteínas de Plantas/metabolismo , Proteínas Quinasas/metabolismo , Transducción de Señal/fisiología , Secuencia de Aminoácidos , Calcineurina/genética , Proteínas de Unión al Calcio/clasificación , Proteínas de Unión al Calcio/genética , Evolución Molecular , Datos de Secuencia Molecular , Filogenia , Proteínas de Plantas/clasificación , Proteínas de Plantas/genética , Proteínas Quinasas/genética , Alineación de SecuenciaRESUMEN
The protein family of membrane-anchored extended synaptotagmin-like proteins (E-Syts) was recently discovered in humans. E-Syt1 to 3 each contain at least one transmembrane domain and three or five C2 domains. To investigate the whole C2 area of murine E-Syt2, highly pure recombinant E-Syt2 (rE-Syt2) covering all three C2 domains was isolated. The structure of rE-Syt2 was studied by small-angle X-ray scattering (SAXS) providing a three-dimensional image of a protein with three C2 domains. Calcium binding of rE-Syt2 triggered structural rearrangements and initiated reversible multimerization of the protein in vitro. Quantitative analysis of the calcium binding revealed an apparent binding constant of 100muM. This is the first structural study of a multi-C2 protein, presumably involved in Ca-dependent signalling events.
Asunto(s)
Proteínas de Unión al Calcio/química , Proteínas de la Membrana/química , Modelos Moleculares , Animales , Calcio/química , Proteínas de Unión al Calcio/clasificación , Proteínas de Unión al Calcio/genética , Secuencia Conservada , Humanos , Proteínas de la Membrana/clasificación , Proteínas de la Membrana/genética , Ratones , Filogenia , Estructura Secundaria de Proteína , Estructura Terciaria de Proteína , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Dispersión del Ángulo Pequeño , Solubilidad , Difracción de Rayos XRESUMEN
Calponin is an actin filament-associated regulatory protein expressed in smooth muscle and non-muscle cells. Calponin is an inhibitor of the actin-activated myosin ATPase. Three isoforms of calponin have been found in the vertebrates. Whereas the role of calponin in regulating smooth muscle contractility has been extensively investigated, the function and regulation of calponin in non-muscle cells is much less understood. Based on recent progresses in the field, this review focuses on the studies of calponin in non-muscle cells, especially its regulation by cytoskeleton tension and function in cell motility. The ongoing research has demonstrated that calponin plays a regulatory role in non-muscle cell motility. Therefore, non-muscle calponin is an attractive target for the control of cell proliferation, migration and phagocytosis, and the treatment of cancer metastasis.
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Proteínas de Unión al Calcio/metabolismo , Citoesqueleto/fisiología , Proteínas de Microfilamentos/metabolismo , Actinas/metabolismo , Secuencia de Aminoácidos , Animales , Proteínas de Unión al Calcio/clasificación , Proteínas de Unión al Calcio/genética , Movimiento Celular , Humanos , Proteínas de Microfilamentos/clasificación , Proteínas de Microfilamentos/genética , Datos de Secuencia Molecular , Células Musculares/enzimología , Músculo Liso/enzimología , Miosinas/metabolismo , Filogenia , Isoformas de Proteínas , Relación Estructura-Actividad , CalponinasRESUMEN
Calcyphosine is an EF-hand protein involved in both Ca(2+)-phosphatidylinositol and cyclic AMP signal cascades, as well as in other cellular functions. The crystal structure of Ca(2+)-loaded calcyphosine was determined up to 2.65 A resolution and reveals a protein containing two pairs of Ca(2+)-binding EF-hand motifs. Calcyphosine shares a highly similar overall topology with calmodulin. However, there are striking differences between EF-hand 4, both N-terminal and C-terminal regions, and interdomain linkers. The C-terminal domain of calcyphosine possesses a large hydrophobic pocket in the presence of calcium ions that might be implicated in ligand binding, while its N-terminal hydrophobic pocket is almost shielded by an additional terminal helix. Calcyphosine is largely monomeric, regardless of the presence of Ca(2+). Differences in structure, oligomeric state in the presence and in the absence of Ca(2+), a highly conserved sequence with low similarity to other proteins, and phylogeny define a new EF-hand-containing family of calcyphosine proteins that extends from arthropods to humans.
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Proteínas de Unión al Calcio/química , Motivos EF Hand , Estructura Secundaria de Proteína , Estructura Terciaria de Proteína , Proteínas Recombinantes/química , Secuencia de Aminoácidos , Animales , Sitios de Unión , Calcio/metabolismo , Proteínas de Unión al Calcio/clasificación , Proteínas de Unión al Calcio/genética , Proteínas de Unión al Calcio/metabolismo , Calmodulina/química , Calmodulina/clasificación , Calmodulina/genética , Calmodulina/metabolismo , Cristalografía por Rayos X , Evolución Molecular , Humanos , Modelos Moleculares , Datos de Secuencia Molecular , Resonancia Magnética Nuclear Biomolecular , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Alineación de Secuencia , Homología de Secuencia de AminoácidoRESUMEN
Centrins are Ca(2+)-binding proteins that have been implicated in a number of biological processes, including organelle duplication, mRNA export, DNA repair and signal transduction. In the protozoan parasite Trypanosoma brucei we have previously described TbCentrin2, which is present on a bi-lobed structure, and involved in the duplication and segregation of the Golgi complex. Recently, another centrin, TbCentrin4, was also found at the bi-lobe and has been implicated in organelle segregation and cytokinesis. We now show that cytokinesis is not inhibited, but that a dysregulation of nuclear and cell division leads to the production of zoids - daughter siblings that contain all organelles except the nucleus. Our results, therefore, suggest that TbCentrin4 is involved in processes that coordinate karyokinesis and cytokinesis.
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Proteínas de Unión al Calcio/metabolismo , División Celular/fisiología , Núcleo Celular/metabolismo , Proteínas Protozoarias/metabolismo , Trypanosoma brucei brucei , Animales , Proteínas de Unión al Calcio/clasificación , Proteínas de Unión al Calcio/genética , Calmodulina/genética , Calmodulina/metabolismo , Filogenia , Isoformas de Proteínas/genética , Isoformas de Proteínas/metabolismo , Proteínas Protozoarias/clasificación , Proteínas Protozoarias/genética , Trypanosoma brucei brucei/citología , Trypanosoma brucei brucei/fisiologíaRESUMEN
The mineralized skeleton is a critical innovation that evolved early in vertebrate history. The tissues found in dermal skeletons of ancient vertebrates are similar to the dental tissues of modern vertebrates; both consist of a highly mineralized surface hard tissue, enamel or enameloid, more resilient body dentin, and basal bone. Many proteins regulating mineralization of these tissues are evolutionarily related and form the secretory calcium-binding phosphoprotein (SCPP) family. We hypothesize here the duplication histories of SCPP genes and their common ancestors, SPARC and SPARCL1. At around the same time that Paleozoic jawless vertebrates first evolved mineralized skeleton, SPARCL1 arose from SPARC by whole genome duplication. Then both before and after the split of ray-finned fish and lobe-finned fish, tandem gene duplication created two types of SCPP genes, each residing on the opposite side of SPARCL1. One type was subsequently used in surface tissue and the other in body tissue. In tetrapods, these two types of SCPP genes were separated by intrachromosomal rearrangement. While new SCPP genes arose by duplication, some old genes were eliminated from the genome. As a consequence, phenogenetic drift occurred: while mineralized skeleton is maintained by natural selection, the underlying genetic basis has changed.