RESUMEN
The emergence of multidrug-resistant (MDR) bacteria has risen rapidly, leading to a great threat to global public health. A promising solution to this problem is the exploitation of phage endolysins. In the present study, a putative N-acetylmuramoyl-L-alanine type-2 amidase (NALAA-2, EC 3.5.1.28) from Propionibacterium bacteriophage PAC1 was characterized. The enzyme (PaAmi1) was cloned into a T7 expression vector and expressed in E. coli BL21 cells. Kinetics analysis using turbidity reduction assays allowed the determination of the optimal conditions for lytic activity against a range of Gram-positive and negative human pathogens. The peptidoglycan degradation activity of PaAmi1 was confirmed using isolated peptidoglycan from P. acnes. The antibacterial activity of PaAmi1 was investigated using live P. acnes cells growing on agar plates. Two engineered variants of PaAmi1 were designed by fusion to its N-terminus two short antimicrobial peptides (AMPs). One AMP was selected by searching the genomes of Propionibacterium bacteriophages using bioinformatics tools, whereas the other AMP sequence was selected from the antimicrobial peptide databases. Both engineered variants exhibited improved lytic activity towards P. acnes and the enterococci species Enterococcus faecalis and Enterococcus faecium. The results of the present study suggest that PaAmi1 is a new antimicrobial agent and provide proof of concept that bacteriophage genomes are a rich source of AMP sequences that can be further exploited for designing novel or improved endolysins.
Asunto(s)
Bacteriófagos , Siphoviridae , Humanos , Propionibacterium acnes/genética , Peptidoglicano/metabolismo , Escherichia coli/metabolismo , Endopeptidasas/metabolismo , Siphoviridae/metabolismo , Bacteriófagos/metabolismo , Antibacterianos/químicaRESUMEN
Bacillus anthracis is a potent biowarfare agent, able to be highly lethal. The bacteria dwell in the soil of certain regions, as natural flora. Bacteriophages or their lytic enzymes, endolysins, may be an alternative for antibiotics and other antibacterials to fight this pathogen in infections and to minimize environmental contamination with anthrax endospores. Upon screening environmental samples from various regions in Poland, we isolated three new siphophages, J5a, F16Ba, and z1a, specific for B. anthracis. They represent new species related to historical anthrax phages Gamma, Cherry, and Fah, and to phage Wbeta of Wbetavirus genus. We show that the new phages and their closest relatives, phages Tavor_SA, Negev_SA, and Carmel_SA, form a separate clade of the Wbetavirus genus, designated as J5a clade. The most distinctive feature of J5a clade phages is their cell lysis module. While in the historical phages it encodes a canonical endolysin and a class III holin, in J5a clade phages it encodes an endolysin with a signal peptide and two putative holins. We present the basic characteristic of the isolated phages. Their comparative genomic analysis indicates that they encode two receptor-binding proteins, of which one may bind a sugar moiety of B. anthracis cell surface.
Asunto(s)
Bacillus anthracis/virología , Bacteriófagos/aislamiento & purificación , Siphoviridae/aislamiento & purificación , Bacillus anthracis/genética , Bacillus anthracis/metabolismo , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Bacteriófagos/clasificación , Bacteriófagos/genética , Bacteriófagos/metabolismo , Genoma Viral , Genómica , Filogenia , Receptores Virales/genética , Receptores Virales/metabolismo , Siphoviridae/clasificación , Siphoviridae/genética , Siphoviridae/metabolismo , Proteínas Virales/genética , Proteínas Virales/metabolismoRESUMEN
Marine viral sequence space is immense and presents a promising resource for the discovery of new enzymes interesting for research and biotechnology. However, bottlenecks in the functional annotation of viral genes and soluble heterologous production of proteins hinder access to downstream characterization, subsequently impeding the discovery process. While commonly utilized for the heterologous expression of prokaryotic genes, codon adjustment approaches have not been fully explored for viral genes. Herein, the sequence-based identification of a putative prophage is reported from within the genome of Hypnocyclicus thermotrophus, a Gram-negative, moderately thermophilic bacterium isolated from the Seven Sisters hydrothermal vent field. A prophage-associated gene cluster, consisting of 46 protein coding genes, was identified and given the proposed name Hypnocyclicus thermotrophus phage H1 (HTH1). HTH1 was taxonomically assigned to the viral family Siphoviridae, by lowest common ancestor analysis of its genome and phylogeny analyses based on proteins predicted as holin and DNA polymerase. The gene neighbourhood around the HTH1 lytic cassette was found most similar to viruses infecting Gram-positive bacteria. In the HTH1 lytic cassette, an N-acetylmuramoyl-L-alanine amidase (Amidase_2) with a peptidoglycan binding motif (LysM) was identified. A total of nine genes coding for enzymes putatively related to lysis, nucleic acid modification and of unknown function were subjected to heterologous expression in Escherichia coli. Codon optimization and codon harmonization approaches were applied in parallel to compare their effects on produced proteins. Comparison of protein yields and thermostability demonstrated that codon optimization yielded higher levels of soluble protein, but codon harmonization led to proteins with higher thermostability, implying a higher folding quality. Altogether, our study suggests that both codon optimization and codon harmonization are valuable approaches for successful heterologous expression of viral genes in E. coli, but codon harmonization may be preferable in obtaining recombinant viral proteins of higher folding quality.
Asunto(s)
Escherichia coli/genética , Fusobacterias/virología , Profagos/metabolismo , Proteínas Virales/metabolismo , Clonación Molecular , Codón , Genoma Viral , Respiraderos Hidrotermales/microbiología , Familia de Multigenes , Filogenia , Profagos/clasificación , Profagos/genética , Pliegue de Proteína , Estabilidad Proteica , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismo , Siphoviridae/clasificación , Siphoviridae/genética , Siphoviridae/metabolismo , Temperatura de Transición , Proteínas Virales/química , Proteínas Virales/genética , Proteínas Virales/aislamiento & purificaciónRESUMEN
A novel siphovirus, vB_PagS_MED16 (MED16) was isolated in Lithuania using Pantoea agglomerans strain BSL for the phage propagation. The double-stranded DNA genome of MED16 (46,103 bp) contains 73 predicted open reading frames (ORFs) encoding proteins, but no tRNA. Our comparative sequence analysis revealed that 26 of these ORFs code for unique proteins that have no reliable identity when compared to database entries. Based on phylogenetic analysis, MED16 represents a new genus with siphovirus morphology. In total, 35 MED16 ORFs were given a putative functional annotation, including those coding for the proteins responsible for virion morphogenesis, phage-host interactions, and DNA metabolism. In addition, a gene encoding a preQ0 DNA deoxyribosyltransferase (DpdA) is present in the genome of MED16 and the LC-MS/MS analysis indicates 2'-deoxy-7-amido-7-deazaguanosine (dADG)-modified phage DNA, which, to our knowledge, has never been experimentally validated in genomes of Pantoea phages. Thus, the data presented in this study provide new information on Pantoea-infecting viruses and offer novel insights into the diversity of DNA modifications in bacteriophages.
Asunto(s)
ADN Viral , Genoma Viral , Guanosina , Sistemas de Lectura Abierta , Pantoea/virología , Siphoviridae , Proteínas Virales , ADN Viral/genética , ADN Viral/metabolismo , Guanosina/análogos & derivados , Guanosina/química , Guanosina/metabolismo , Siphoviridae/genética , Siphoviridae/metabolismo , Proteínas Virales/genética , Proteínas Virales/metabolismoRESUMEN
Bacteriophages can play beneficial roles in phage therapy and destruction of food pathogens. Conversely, they play negative roles as they infect bacteria involved in fermentation, resulting in serious industrial losses. Siphoviridae phages possess a long non-contractile tail and use a mechanism of infection whose first step is host recognition and binding. They have evolved adhesion devices at their tails' distal end, tuned to recognize specific proteinaceous or saccharidic receptors on the host's surface that span a large spectrum of shapes. In this review, we aimed to identify common patterns beyond this apparent diversity. To this end, we analyzed siphophage tail tips or baseplates, evaluating their known structures, where available, and uncovering patterns with bioinformatics tools when they were not. It was thereby identified that a triad formed by three proteins in complex, i.e., the tape measure protein (TMP), the distal tail protein (Dit), and the tail-associated lysozyme (Tal), is conserved in all phages. This common scaffold may harbor various functional extensions internally while it also serves as a platform for plug-in ancillary or receptor-binding proteins (RBPs). Finally, a group of siphophage baseplates involved in saccharidic receptor recognition exhibits an activation mechanism reminiscent of that observed in Myoviridae.
Asunto(s)
Proteínas Bacterianas/metabolismo , Bacteriófagos/metabolismo , Lactococcus lactis/metabolismo , Siphoviridae/metabolismo , Proteínas de la Cola de los Virus/química , Proteínas de la Cola de los Virus/metabolismo , Proteínas Bacterianas/química , Proteínas Bacterianas/genética , Bacteriófagos/química , Bacteriófagos/genética , Cristalografía por Rayos X , Lactococcus lactis/química , Lactococcus lactis/genética , Lactococcus lactis/virología , Receptores Virales/genética , Siphoviridae/química , Siphoviridae/genética , Proteínas de la Cola de los Virus/genéticaRESUMEN
The lytic cassette of Lactococcus lactis prophage TP712 contains a putative membrane protein of unknown function (Orf54), a holin (Orf55), and a modular endolysin with a N-terminal glycoside hydrolase (GH_25) catalytic domain and two C-terminal LysM domains (Orf56, LysTP712). In this work, we aimed to study the mode of action of the endolysin LysTP712. Inducible expression of the holin-endolysin genes seriously impaired growth. The growth of lactococcal cells overproducing the endolysin LysTP712 alone was only inhibited upon the dissipation of the proton motive force by the pore-forming bacteriocin nisin. Processing of a 26-residues signal peptide is required for LysTP712 activation, since a truncated version without the signal peptide did not impair growth after membrane depolarization. Moreover, only the mature enzyme displayed lytic activity in zymograms, while no lytic bands were observed after treatment with the Sec inhibitor sodium azide. LysTP712 might belong to the growing family of multimeric endolysins. A C-terminal fragment was detected during the purification of LysTP712. It is likely to be synthesized from an alternative internal translational start site located upstream of the cell wall binding domain in the lysin gene. Fractions containing this fragment exhibited enhanced activity against lactococcal cells. However, under our experimental conditions, improved in vitro inhibitory activity of the enzyme was not observed upon the supplementation of additional cell wall binding domains in. Finally, our data pointed out that changes in the lactococcal cell wall, such as the degree of peptidoglycan O-acetylation, might hinder the activity of LysTP712. LysTP712 is the first secretory endolysin from a lactococcal phage described so far. The results also revealed how the activity of LysTP712 might be counteracted by modifications of the bacterial peptidoglycan, providing guidelines to exploit the biotechnological potential of phage endolysins within industrially relevant lactococci and, by extension, other bacteria.
Asunto(s)
Endopeptidasas/metabolismo , Lactococcus lactis/virología , Profagos/fisiología , Siphoviridae/fisiología , Acetilación , Bacteriólisis/efectos de los fármacos , Pared Celular/metabolismo , Endopeptidasas/química , Endopeptidasas/genética , Lactococcus lactis/crecimiento & desarrollo , Lactococcus lactis/metabolismo , Nisina/farmacología , Peptidoglicano/metabolismo , Profagos/genética , Profagos/metabolismo , Dominios Proteicos , Señales de Clasificación de Proteína , Siphoviridae/genética , Siphoviridae/metabolismoRESUMEN
Cyanobacteria are the most abundant photosynthetic microorganisms, the global distribution of which is mainly regulated by the corresponding cyanophages. A systematic screening of water samples in the Lake Chaohu enabled us to isolate a freshwater siphocyanophage that infects Microcystis wesenbergii, thus termed Mic1. Using cryoelectron microscopy, we solved the 3.5-Å structure of Mic1 capsid. The major capsid protein gp40 of an HK97-like fold forms two types of capsomers, hexons and pentons. The capsomers interact with each other via the interweaved N-terminal arms of gp40 in addition to a tail-in-mouth joint along the three-fold symmetric axis, resulting in the assembly of capsid in a mortise-and-tenon pattern. The novel-fold cement protein gp47 sticks at the two-fold symmetric axis and further fixes the capsid. These findings provide structural insights into the assembly of cyanophages, and set up a platform to explore the mechanism of specific interactions and co-evolution with cyanobacteria.
Asunto(s)
Proteínas de la Cápside/química , Proteínas de la Cápside/metabolismo , Microcystis/virología , Siphoviridae/metabolismo , Microscopía por Crioelectrón , Modelos Moleculares , Conformación Proteica , Dominios Proteicos , Pliegue de Proteína , Multimerización de Proteína , Siphoviridae/químicaRESUMEN
We present the genome sequences of Salmonella enterica tailed phages Sasha, Sergei, and Solent. These phages, along with Salmonella phages 9NA, FSL_SP-062, and FSL_SP-069 and the more distantly related Proteus phage PmiS-Isfahan, have similarly sized genomes of between 52 and 57 kbp in length that are largely syntenic. Their genomes also show substantial genome mosaicism relative to one another, which is common within tailed phage clusters. Their gene content ranges from 80 to 99 predicted genes, of which 40 are common to all seven and form the core genome, which includes all identifiable virion assembly and DNA replication genes. The total number of gene types (pangenome) in the seven phages is 176, and 59 of these are unique to individual phages. Their core genomes are much more closely related to one another than to the genome of any other known phage, and they comprise a well-defined cluster within the family Siphoviridae To begin to characterize this group of phages in more experimental detail, we identified the genes that encode the major virion proteins and examined the DNA packaging of the prototypic member, phage 9NA. We show that it uses a pac site-directed headful packaging mechanism that results in virion chromosomes that are circularly permuted and about 13% terminally redundant. We also show that its packaging series initiates with double-stranded DNA cleavages that are scattered across a 170-bp region and that its headful measuring device has a precision of ±1.8%.IMPORTANCE The 9NA-like phages are clearly highly related to each other but are not closely related to any other known phage type. This work describes the genomes of three new 9NA-like phages and the results of experimental analysis of the proteome of the 9NA virion and DNA packaging into the 9NA phage head. There is increasing interest in the biology of phages because of their potential for use as antibacterial agents and for their ecological roles in bacterial communities. 9NA-like phages that infect two bacterial genera have been identified to date, and related phages infecting additional Gram-negative bacterial hosts are likely to be found in the future. This work provides a foundation for the study of these phages, which will facilitate their study and potential use.
Asunto(s)
Empaquetamiento del ADN/genética , Fagos de Salmonella/genética , Salmonella/virología , Empaquetamiento del ADN/fisiología , Replicación del ADN , ADN Viral/genética , Genoma/genética , Genoma Viral/genética , Genómica/métodos , Filogenia , Salmonella/genética , Salmonella/metabolismo , Siphoviridae/genética , Siphoviridae/metabolismo , Proteínas Virales/genética , Virión/genéticaRESUMEN
A novel low-temperature siphovirus, vB_PagS_Vid5 (Vid5), was isolated in Lithuania using Pantoea agglomerans isolate for the phage propagation. The 61,437 bp genome of Vid5 has a Gâ»C content of 48.8% and contains 99 probable protein encoding genes and one gene for tRNASer. A comparative sequence analysis revealed that 46 out of 99 Vid5 open reading frames (ORFs) code for unique proteins that have no reliable identity to database entries. In total, 33 Vid5 ORFs were given a putative functional annotation, including those coding for the proteins responsible for virion morphogenesis, phage-host interactions, and DNA metabolism. In addition, a cluster of genes possibly involved in the biosynthesis of 7-deazaguanine derivatives was identified. Notably, one of these genes encodes a putative preQ0/preQ1 transporter, which has never been detected in bacteriophages to date. A proteomic analysis led to the experimental identification of 11 virion proteins, including nine that were predicted by bioinformatics approaches. Based on the phylogenetic analysis, Vid5 cannot be assigned to any genus currently recognized by ICTV, and may represent a new one within the family of Siphoviridae.
Asunto(s)
Bacteriófagos/genética , Bacteriófagos/metabolismo , Pantoea/virología , Bacteriófagos/ultraestructura , Frío , Biología Computacional , Genes Virales , Genoma Viral , Genómica/métodos , Guanosina/análogos & derivados , Guanosina/biosíntesis , Especificidad del Huésped , Familia de Multigenes , Filogenia , Proteómica/métodos , Análisis de Secuencia de ADN , Siphoviridae/genética , Siphoviridae/metabolismo , Proteínas Virales/genética , Proteínas Virales/metabolismoRESUMEN
With increasing numbers of 3D structures of bacteriophage components, combined with powerful in silico predictive tools, it has become possible to decipher the structural assembly and associated functionality of phage adhesion devices. Recently, decorations have been reported in the tail and neck passage structures of members of the so-called 936 group of lactococcal siphophages. In the current report, using bioinformatic analysis we identified a conserved carbohydrate binding module (CBM) among many of the virion baseplate Dit components, in addition to the CBM present in the 'classical' receptor binding proteins (RBPs). We observed that, within these so-called 'evolved' Dit proteins, the identified CBMs have structurally conserved folds, yet can be grouped into four distinct classes. We expressed such modules in fusion with GFP, and demonstrated their binding capability to their specific host using fluorescent binding assays with confocal microscopy. We detected evolved Dits in several phages infecting various Gram-positive bacterial species, including mycobacteria. The omnipresence of CBM domains in siphophages indicates their auxiliary role in infection, as they can assist in the specific recognition of and attachment to their host, thus ensuring a highly efficient and specific phage-host adhesion process as a prelude to DNA injection.
Asunto(s)
Lactococcus lactis/virología , Siphoviridae/genética , Siphoviridae/metabolismo , Proteínas de la Cola de los Virus/genética , Virión/genética , Carbohidratos/química , Modelos Moleculares , Unión Proteica , Conformación ProteicaRESUMEN
Psychrotrophic gram-negative Pseudomonas spp. represent a serious problem in the dairy industry as they can cause spoilage of milk and dairy products. Bacteriophages have moved into focus as promising biocontrol agents for such food spoilage bacteria. The virulent Siphoviridae phage PMBT14 was isolated on a mutant variant of P. fluorescens DSM 50090 challenged with an unrelated virulent P. fluorescens DSM 50090 Podoviridae phage (i.e., mutant strain DSM 50090R). PMBT14 has a 47,820-bp dsDNA genome with 76 predicted open reading frames (ORFs). Its genome shows no significant sequence similarity to that of known phages, suggesting that PMBT14 represents a novel phage. Phage PMBT14 could be a promising biocontrol agent for P. fluorescens in milk or dairy foods.
Asunto(s)
Genoma Viral , Lisogenia/fisiología , Fagos Pseudomonas/genética , Pseudomonas fluorescens/virología , Siphoviridae/genética , Proteínas Virales/genética , Agentes de Control Biológico , Mapeo Cromosómico , ADN/genética , ADN/metabolismo , ADN Viral/genética , ADN Viral/metabolismo , Productos Lácteos/microbiología , Microbiología de Alimentos , Ontología de Genes , Tamaño del Genoma , Humanos , Anotación de Secuencia Molecular , Sistemas de Lectura Abierta , Filogenia , Fagos Pseudomonas/clasificación , Fagos Pseudomonas/patogenicidad , Fagos Pseudomonas/ultraestructura , Análisis de Secuencia de ADN , Siphoviridae/clasificación , Siphoviridae/metabolismo , Proteínas Virales/metabolismoRESUMEN
Bacteriophages represent the widest group of viruses, from which only virulent phages are used as antibacterial agent. But the picture in the case of temperate phage is absolutely different; many lysogenic phages express gene products that have subtle effects on the phenotype of the host cell. This process is called lysogenic conversion. In present study we characterized new temperate Enterococcus faecium phage vB_GEC_EFS_2, which was isolated from river Mtkvari. The phage is a member of Siphoviridae family. Whole genome of phage vB_GEC-EfS_2 was sequenced and analyzed. Total length of the genome of phage vB_GEC_EFS_2 is 38 508bp, The assembly contains 65 ORFs, among them - 3 lysis genes , genes coded 13 structural proteins, 1 DNA replication-associated gene, 1 gene coded integration, 3 - lysis-lysogenic cycle regulation, 43 hypothetical proteins. One holin gene contained "Haemolysin XhIA" domain which is surface associated haemolisyn. We isolated and purified holin gene and determine its haemolitic activity alongside with vB_GEC_EfS_2 phage lysate. We clarified the XhIA domain function and role in protein's haemolytic nature and described another kind of lysogenic conversion.
Asunto(s)
Bacteriófagos/genética , ADN Viral/genética , Genoma Viral , Proteínas Hemolisinas/genética , Lisogenia , Siphoviridae/genética , Bacteriófagos/clasificación , Bacteriófagos/aislamiento & purificación , Bacteriófagos/metabolismo , Clonación Molecular , ADN Viral/metabolismo , Enterococcus faecium/virología , Escherichia coli/genética , Escherichia coli/metabolismo , Expresión Génica , Vectores Genéticos/química , Vectores Genéticos/metabolismo , Tamaño del Genoma , Georgia (República) , Proteínas Hemolisinas/metabolismo , Secuenciación de Nucleótidos de Alto Rendimiento , Sistemas de Lectura Abierta , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Ríos/virología , Siphoviridae/clasificación , Siphoviridae/aislamiento & purificación , Siphoviridae/metabolismoRESUMEN
Marine bacteriophage TW1 belongs to the Siphoviridae family and infects Pseudoalteromonas phenolica. Mass spectrometry analysis has identified 16 different proteins in the TW1 virion. Functions of most of these proteins have been predicted by bioinformatic methods. A 3.6 Å resolution cryoelectron microscopy map of the icosahedrally averaged TW1 head showed the atomic structures of the major capsid protein, gp57∗, and the capsid-stabilizing protein, gp56. The gp57∗ structure is similar to that of the phage HK97 capsid protein. The gp56 protein has two domains, each having folds similar to that of the N-terminal part of phage λ gpD, indicating a common ancestry. The first gp56 domain clamps adjacent capsomers together, whereas the second domain is required for trimerization. A 6-fold-averaged reconstruction of the distal part of the tail showed that TW1 has six tail spikes, which are unusual for siphophages but are similar to the podophages P22 and Sf6, suggesting a common evolutionary origin of these spikes.
Asunto(s)
Proteínas de la Cápside/metabolismo , Cápside/metabolismo , Siphoviridae/metabolismo , Bacteriófagos/metabolismo , Espectrometría de Masas , Modelos Moleculares , Conformación Proteica , Ensamble de VirusRESUMEN
To establish a prophage state, the genomic DNA of temperate bacteriophages normally becomes integrated into the genome of their host bacterium by integrase-mediated, site-specific DNA recombination. Serine integrases catalyse a single crossover between an attachment site in the host (attB) and a phage attachment site (attP) on the circularized phage genome to generate the integrated prophage DNA flanked by recombinant attachment sites, attL and attR. Exiting the prophage state and entry into the lytic growth cycle requires an additional phage-encoded protein, the recombination directionality factor or RDF, to mediate recombination between attL and attR and excision of the phage genome. The RDF is known to bind integrase and switch its activity from integration (attP x attB) to excision (attL x attR) but its precise mechanism is unclear. Here, we identify amino acid residues in the RDF, gp3, encoded by the Streptomyces phage ÏC31 and within the ÏC31 integrase itself that affect the gp3:Int interaction. We show that residue substitutions in integrase that reduce gp3 binding adversely affect both excision and integration reactions. The mutant integrase phenotypes are consistent with a model in which the RDF binds to a hinge region at the base of the coiled-coil motif in ÏC31 integrase.
Asunto(s)
Sitios de Ligazón Microbiológica , ADN Bacteriano/química , Proteínas de Unión al ADN/química , Integrasas/química , Siphoviridae/genética , Streptomyces/virología , Proteínas Virales/química , Secuencia de Aminoácidos , Sustitución de Aminoácidos , Sitios de Unión , Clonación Molecular , ADN Bacteriano/genética , ADN Bacteriano/metabolismo , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/metabolismo , Escherichia coli/genética , Escherichia coli/metabolismo , Expresión Génica , Vectores Genéticos/química , Vectores Genéticos/metabolismo , Integrasas/genética , Integrasas/metabolismo , Lisogenia , Modelos Moleculares , Mutación , Unión Proteica , Conformación Proteica en Hélice alfa , Conformación Proteica en Lámina beta , Dominios y Motivos de Interacción de Proteínas , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Siphoviridae/química , Siphoviridae/metabolismo , Streptomyces/química , Termodinámica , Proteínas Virales/genética , Proteínas Virales/metabolismoRESUMEN
Assembling multiple DNA fragments into functional plasmids is an important and often rate-limiting step in engineering new functions in living systems. Bacteriophage integrases are enzymes that carry out efficient recombination reactions between short, defined DNA sequences known as att sites. These DNA splicing reactions can be used to assemble large numbers of DNA fragments into a functional circular plasmid in a method termed serine integrase recombinational assembly (SIRA). The resulting DNA assemblies can easily be modified by further recombination reactions catalyzed by the same integrase in the presence of its recombination directionality factor (RDF). Here we present a set of protocols for the overexpression and purification of bacteriophage ÏC31 and Bxb1 integrase and RDF proteins, their use in DNA assembly reactions, and subsequent modification of the resulting DNA assemblies.
Asunto(s)
ADN Nucleotidiltransferasas/genética , Integrasas/genética , Ingeniería Metabólica/métodos , Plásmidos/metabolismo , Siphoviridae/genética , Proteínas Virales/genética , Sitios de Ligazón Microbiológica , ADN Nucleotidiltransferasas/aislamiento & purificación , ADN Nucleotidiltransferasas/metabolismo , ADN Circular/genética , ADN Circular/metabolismo , ADN Viral/genética , ADN Viral/metabolismo , Escherichia coli/genética , Escherichia coli/metabolismo , Expresión Génica , Integrasas/aislamiento & purificación , Integrasas/metabolismo , Plásmidos/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/aislamiento & purificación , Proteínas Recombinantes/metabolismo , Recombinación Genética , Serina/metabolismo , Siphoviridae/metabolismo , Proteínas Virales/aislamiento & purificación , Proteínas Virales/metabolismoRESUMEN
Minicircle DNA vectors are plasmid derivatives free of bacterial elements. These vectors are mostly provided from common plasmids via recombination by site-specific recombinases in E. coli. Absence of bacterial backbone in minicircle vectors results in high-level and persistent expression of transgene in comparison with conventional plasmids and provides promising vehicles for gene therapy and vaccination. Here we describe the production of replicative minicircle DNA vectors using the PBAD/araC system expressing ΦC31 integrase in E. coli.
Asunto(s)
ADN Nucleotidiltransferasas/genética , ADN Circular/genética , Escherichia coli/genética , Ingeniería Genética/métodos , Siphoviridae/genética , Transfección/métodos , Animales , Sitios de Ligazón Microbiológica , Células CHO , Cricetulus , ADN Nucleotidiltransferasas/metabolismo , ADN Circular/metabolismo , Escherichia coli/metabolismo , Expresión Génica , Genes Reporteros , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Plásmidos/química , Plásmidos/metabolismo , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Recombinación Genética , Siphoviridae/metabolismo , TransgenesRESUMEN
The tail tape measure protein (TMP) of tailed bacteriophages (also called phages) dictates the tail length and facilitates DNA transit to the cell cytoplasm during infection. Here, a thorough mutational analysis of the TMP from lactococcal phage TP901-1 (TMPTP901-1) was undertaken. We generated 56 mutants aimed at defining TMPTP901-1 domains that are essential for tail assembly and successful infection. Through analysis of the derived mutants, we determined that TP901-1 infectivity requires the N-terminal 154 aa residues, the C-terminal 60 residues and the first predicted hydrophobic region of TMPTP901-1 as a minimum. Furthermore, the role of TMPTP901-1 in tail length determination was visualized by electron microscopic imaging of TMP-deletion mutants. The inverse linear correlation between the extent of TMPTP901-1-encoding gene deletions and tail length of the corresponding virion provides an estimate of TMPTP901-1 regions interacting with the connector or involved in initiator complex formation. This study represents the most thorough characterisation of a TMP from a Gram-positive host-infecting phage and provides essential advances to understanding its role in virion assembly, morphology and infection.
Asunto(s)
Lactococcus/virología , Siphoviridae/química , Proteínas Virales/química , Siphoviridae/metabolismo , Relación Estructura-Actividad , Proteínas Virales/metabolismo , Proteínas Virales/ultraestructuraRESUMEN
Bacteria in the Roseobacter lineage have been studied extensively due to their significant biogeochemical roles in the marine ecosystem. However, our knowledge on bacteriophage which infects the Roseobacter clade is still very limited. Here, we report a new bacteriophage, phage DSS3Φ8, which infects marine roseobacter Ruegeria pomeroyi DSS-3. DSS3Φ8 is a lytic siphovirus. Genomic analysis showed that DSS3Φ8 is most closely related to a group of siphoviruses, CbK-like phages, which infect freshwater bacterium Caulobacter crescentus. DSS3Φ8 contains a smaller capsid and has a reduced genome size (146 kb) compared to the CbK-like phages (205-279 kb). DSS3Φ8 contains the DNA polymerase gene which is closely related to T7-like podoviruses. DSS3Φ8 also contains the integrase and repressor genes, indicating its potential to involve in lysogenic cycle. In addition, four GTA (gene transfer agent) genes were identified in the DSS3Φ8 genome. Genomic analysis suggests that DSS3Φ8 is a highly mosaic phage that inherits the genetic features from siphoviruses, podoviruses, prophages and GTAs. This is the first report of CbK-like phages infecting marine bacteria. We believe phage isolation is still a powerful tool that can lead to discovery of new phages and help interpret the overwhelming unknown sequences in the viral metagenomics.
Asunto(s)
Filogenia , Roseobacter/virología , Siphoviridae/genética , Proteínas de la Cápside/genética , Proteínas de la Cápside/metabolismo , Genoma Viral , Lisogenia , Siphoviridae/clasificación , Siphoviridae/metabolismo , Siphoviridae/fisiologíaRESUMEN
M. tuberculosis is intrinsically tolerant to many antibiotics largely due to the imperviousness of its unusual mycolic acid-containing cell wall to most antimicrobials. The emergence and increasingly widespread of multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant tuberculosis (XDR-TB) revitalized keen interest in phage-inspired therapy. SWU1gp39 is a novel gene from mycobacteriophage SWU1 with unknown function. SWU1gp39 expressed in M. smegmatis conferred the host cell increased susceptibility to multiple antibiotics, including isoniazid, erythromycin, norfloxacin, ampicillin, ciprofloxacin, ofloxacin, rifampicin and vancomycin, and multiple environment stresses such as H2O2, heat shock, low pH and SDS. By using EtBr/Nile red uptake assays, WT-pAL-gp39 strain showed higher cell wall permeability than control strain WT-pAL. Moreover, the WT-pAL-gp39 strain produced more reactive oxygen species and reduced NAD(+)/NADH ratio. RNA-Seq transcriptomes of the WT-pAL-gp39 and WT-pAL revealed that the transcription of 867 genes was differentially regulated, including genes associated with lipid metabolism. Taken together, our results implicated that SWU1gp39, a novel gene from mycobacteriophage, disrupted the lipid metabolism of host and increased cell wall permeability, ultimately potentiated the efficacy of multiple antibiotics and stresses against mycobacteria.
Asunto(s)
Antituberculosos , Pared Celular , Mycobacterium tuberculosis , Siphoviridae , Proteínas Virales , Antituberculosos/farmacocinética , Antituberculosos/farmacología , Pared Celular/genética , Pared Celular/metabolismo , Pared Celular/virología , Mycobacterium smegmatis/genética , Mycobacterium smegmatis/metabolismo , Mycobacterium smegmatis/virología , Mycobacterium tuberculosis/genética , Mycobacterium tuberculosis/metabolismo , Mycobacterium tuberculosis/virología , Permeabilidad , Siphoviridae/genética , Siphoviridae/metabolismo , Proteínas Virales/genética , Proteínas Virales/metabolismoRESUMEN
Despite the importance of phages in driving horizontal gene transfer (HGT) among pathogenic bacteria, the underlying molecular mechanisms mediating phage adsorption to S. aureus are still unclear. Phage Ï11 is a siphovirus with a high transducing efficiency. Here, we show that the tail protein Gp45 localized within the Ï11 baseplate. Phage Ï11 was efficiently neutralized by anti-Gp45 serum, and its adsorption to host cells was inhibited by recombinant Gp45 in a dose-dependent manner. Flow cytometry analysis demonstrated that biotin-labelled Gp45 efficiently stained the wild-type S. aureus cell but not the double knockout mutant ΔtarM/S, which lacks both α- and ß-O-GlcNAc residues on its wall teichoic acids (WTAs). Additionally, adsorption assays indicate that GlcNAc residues on WTAs and O-acetyl groups at the 6-position of muramic acid residues in peptidoglycan are essential components of the Ï11 receptor. The elucidation of Gp45-involved molecular interactions not only broadens our understanding of siphovirus-mediated HGT, but also lays the groundwork for the development of sensitive affinity-based diagnostics and therapeutics for S. aureus infection.