Multiple Effects of Egg Weight, in Ovo Carbohydrates, and Sex of Birds on Posthatch Performance in Broilers.

Chickens raised for their meat (Gallus gallus domesticus) tend to have a critical phase of life right after hatching due to the management of modern production systems. Early nutrition strategies such as in ovo intervention can be an alternative means to support growth and gut health by compensating for the energy deficit after pipping out of the egg. In the current study, 1200 Ross 308 eggs were used to examine the effects of a complex carbohydrate solution of disaccharides and glucose applied in ovo on hatchability, the hatching time of different-sized eggs, and the development, performance, and carcass characteristics of broilers of both sexes. The eggs were divided into three treatment groups: intact (NT), in ovo saline (ioS), and in ovo carbohydrate mixture (ioCH). The incubation protocol was performed according to the recommendations of Aviagen (2019), and the in ovo process was carried out on day 17 by manually injecting 0.5 mL of the solutions into the amniotic fluid. After hatching, the birds were kept in floor pens until day 35 and fed ad libitum in a three-phase feeding program. Body weight, average daily weight gain, feed intake and conversion, and carcass characteristics were measured during the trial. In ovo carbohydrates reduced hatchability by 15%, while growth performance and the weight of thigh and breast muscle were enhanced significantly (p < 0.05) compared with ioS as a possible outcome of carbohydrate-to-muscle satellite cell proliferation and protein accumulation. However, further study is needed to refine the in ovo carbohydrate supplementation method to minimize the mortality of embryos during hatching.

Evaluation of risk factors affecting strongylid egg shedding on Hungarian horse farms.

In Hungary, equine parasite control is mostly based on traditional approaches involving rotational anthelmintic treatment at regular intervals. Almost no farms utilize fecal egg counts (FECs) on a regular basis to guide deworming treatments. This approach is certainly not sustainable and is the major factor responsible for the current high levels of anthelmintic resistance. The aims of this study were to statistically analyze risk factors associated with strongylid egg count magnitude and prevalence. Fecal samples and horse data (age, pasture history, stocking density, anthelmintic history) were collected from 216 horses on 13 farms in Pest county, Hungary, during the spring of 2020. FECs were determined by Mini-FLOTAC. Logistic regression models were used to evaluate the association of factors with pre-deworming strongylid egg counts. The data were highly overdispersed with approximately 22% (95% Confidence Interval: 17-28%) of the equids shedding 80% of the total strongylid egg output. Strongylid FECs were significantly associated with the age of equids and anthelmintic class used prior to the study. Equids younger than 5 years of age had significantly higher FECs compared to equids in the 5-17-year age range (p = 0.003) and compared to equids aged >17 years (p < 0.001). Equids treated regularly with benzimidazoles had significantly higher FECs than equids with no history of benzimidazole use (p = 0.02). We found that FECs of horses kept at extremely high stocking density (>30 horses/ha) were significantly higher than those kept at low (1-2 horses/ha; p < 0.001) or medium (3-10 horses/ha, p < 0.001) stocking densities. The results demonstrate the value of FEC monitoring and indicate that the efficacy of benzimidazoles should be investigated in Hungary. Moreover our findings demonstrate that reducing stocking density should be considered in cases of high strongylid FECs.

Habitat Characteristics as Potential Drivers of the Angiostrongylus daskalovi Infection in European Badger (Meles meles) Populations.

From 2016 to 2020, an investigation was carried out to identify the rate of Angiostrongylus spp. infections in European badgers in Hungary. During the study, the hearts and lungs of 50 animals were dissected in order to collect adult worms, the morphometrical characteristics of which were used for species identification. PCR amplification and an 18S rDNA-sequencing analysis were also carried out. Global and local spatial autocorrelation methods were used to detect high-rated and low-rated infected animal clusters. We conducted a binary logistic regression analysis along with hierarchical agglomerative clustering to determine the relation between selected biotic and abiotic variables, and the prevalence of an A. daskalovi infection. We found a high prevalence (72%) and moderate mean intensity (14.1) of Angiostrongylus sp. infection. Morphology and sequencing revealed that all animals were infected by A. daskalovi. The results of both spatial autocorrelations suggested that the spatial distribution of infected badgers was more spatially clustered than random. The results of an analysis of the correlation between habitat characteristics and infection showed that the infected animals could be associated with dry and open landscape habitats without extended and connected canopy. It is suggested that the territorial behaviour of badgers and the landscape-directed aggregation of potential intermediate hosts might be the drivers of an A. daskalovi infection.

Effects of Long-Term Selection in the Border Collie Dog Breed: Inbreeding Purge of Canine Hip and Elbow Dysplasia.

Pedigree data of 13,339 border collie dog was collected along with canine hip dysplasia (CHD) and canine elbow dysplasia (CED) records (1352 CHD and 524 CED), and an inbreeding-purging (IP) model was created. Ancestral inbreeding coefficients were calculated by using a gene dropping simulation method with GRain 2.2 software. Cumulative logit models (CLM) for CHD and CED were fitted using a logit-link Poisson distribution and the classical (F_W), and ancestral inbreeding (F_BAL, F_KAL, and F_KAL_NEW) coefficients as linear regression coefficients. The effective population size was calculated from F_W and decreased in the examined period along with an increase of F_W; however, slight differences were found as a consequence of breeding dog imports. CHD values were lowered by the expansion of F_BAL, as the alleles had been inbred in the past. For CHD, signs of purging were obtained. There was a positive trend regarding the breeding activity (both sire and dam of the future litters should be screened and certified free from CHD and CED), as years of selection increased the frequency of alleles with favorable hip and elbow conformation. Division of the ancestral inbreeding coefficient showed that alleles that had been identical by descent (IBD) for the first time (F_KAL_NEW) had a negative effect on both traits, while F_KAL has shown favorable results for alleles IBD in past generations. Some authors had proven this phenomenon in captive populations or experimental conditions; however, no evidence of inbreeding purge has ever been described in dog populations. Despite the various breeding practices, it seems that alleles of these polygenic disorders could be successfully purged out of the population with long-term selection.

Population Structure Analysis of the Border Collie Dog Breed in Hungary.

Pedigree data of the Border Collie dog breed were collected in Hungary to examine genetic diversity within the breed and its different lines. The database was based on available herd books dating from the development of the breed (in the late 1800s) to the present day. The constructed pedigree file consisted of 13,339 individuals, of which 1566 dogs (born between 2010 and 2016) composed the alive reference population which was active from breeding perspective. The breed is subdivided by phenotype, showing a thicker coat, harmonic movement, a wide skull, and heavier bones for the show type, and a thinner or sometimes short coat and smaller body for the working line, while the mixed line is quite heterogeneous (a combination of the above). Thus, the reference population was dissected according to the existing lines. The number of founders was 894, but eight individuals were responsible for contributing 50% of the genetic variability. The reference population had a pedigree completeness of 99.6% up to 15 generations and an inbreeding coefficient of 9.86%. Due to the changing breed standards and the requirements of the potential buyers, the effective population size substantially decreased between 2010 and 2016. Generation intervals varied between 4.09 and 4.71 years, where the sire paths were longer due to the later initial age of breeding in males compared to females. Genetic differences among the existing lines calculated by fixation indices are not significant; nonetheless ancestral inbreeding coefficients are able to show contrasts.