The Effects of Antioxidants and Pulsed Magnetic Fields on Slow and Fast Skeletal Muscle Atrophy Induced by Streptozotocin: A Preclinical Study.

Results: Our findings suggest that antioxidants and PMF may alleviate impaired protein synthesis and degradation pathways in skeletal muscle atrophy. PTS showed a positive effect on the anabolic pathway, while RSV and PMF demonstrated potential for ameliorating the catabolic pathway. Notably, the combination therapy of antioxidants and PMF exhibited a stronger ameliorative effect on skeletal muscle atrophy than either intervention alone. Conclusion: The present results highlight the benefits of employing a multimodal approach, involving both antioxidant and PMF therapy, for the management of muscle-wasting conditions. These treatments may have potential therapeutic implications for skeletal muscle atrophy.

Accumulation and Distribution of Nanoparticles (Al2O3, CuO, and TiO2) in Tissues of Freshwater Mussel (Unio tigridis).

Freshwater mussels are used as an effective bioindicator of metal pollution. There is no data on the accumulation of any metal-oxide nanoparticles (NPs) in tissues of Unio tigridis. Thus, this study was undertaken to investigate accumulation of Al2O3, CuO, and TiO2 NPs following exposure to different concentrations (0, 1, 3, and 9 mg/L) of NPs for 14 days. Metal concentrations in tissues were determined by ICP-MS, while NP presence was demonstrated by transmission electron microscope (TEM) images. During the experiments, mussels were fed with cultured algae (Chlorella vulgaris). TEM images demonstrated the presence of NPs in digestive gland and muscle. TEM images also suggested that NPs were taken via the lysosomes or endosomes. Highest mean concentrations (µg/g d.w.) of aluminium (76.51), copper (111.63) and titanium (113.83) occurred in the gills and followed by the digestive glands and muscles. Algae consumption of mussels did not significantly differ among controls and NP-exposed groups.

Effects of Theranekron and alpha-lipoic acid combined treatment on GAP-43 and Krox-20 gene expressions and inflammation markers in peripheral nerve injury.

Peripheral nerve injury (PNI) is a major health problem that results in loss of motor and sensory functions. In treatment of PNI, various methods such as anastomosis, nerve grafts, nonneural tissue grafts, and nerve conduits are applied. In the present study, it was aimed to investigate the effects of Theranekron and Alpha-lipoic acid (ALA) combined treatment on nerve healing in experimental PNI by using histomorphometric, electron microscopic, immunohistochemical and molecular biological methods. Sixty-two Wistar rats were divided into six groups; the normal control group, sham operation group, experimental control group having a crush type injury with no treatment, Theranekron treatment group, ALA treatment group and Theranekron+ALA combined treatment group. Sciatic nerve tissue samples were obtained on days 1, 7 and 14 following injury in all groups. GAP-43 expression was upregulated in all PNI received groups compared to the control group. Krox-20 expression was downregulated in all groups that received PNI compared to the control group. While intensely positive TNF-α and IL-6 expressions were observed up to the 1st to the 14th day for the experimental control group, these expressions were seen as "weakly positive" in the treatment groups from the 1st day to the 14th day. The number of myelinated fibers was higher in the control and sham operation groups. Additionally, the number of myelinated nerve fibers increased in the combined treatment group. In conclusion, these findings suggest that combined therapy of Theranekron and ALA promotes structural recovery and it should be considered as an effective treatment protocol following PNI.