In vitro cytotoxic effect of PARP inhibitor alone and in combination with nab­paclitaxel on triple­negative and luminal A breast cancer cells.

In the present study, the in vitro cytotoxic effect of poly(ADP­ribose) polymerase (PARP) inhibitor alone and in combination with nab­paclitaxel was evaluated on human triple­negative breast cancer (TNBC) cell line MDA­MB­231 and human luminal A breast cancer cell line MCF­7. For this purpose, cell index (CI) values obtained from xCELLigence Real­Time Cell Analysis (RTCA) DP instrument, mitotic index (MI), labelling index (LI) and apoptotic index (AI) analysis among cell kinetic parameters were used. As a result of PARP inhibitor application, there was a significant decrease in CI, MI and LI and a significant increase in AI for all the experimental groups. After application of PARP inhibitor in combination with nab­paclitaxel, the CI values were decreased for both cell lines, and the differences between the control and all the experimental groups were statistically significant (P<0.01) for all applications. PARP inhibitor, alone or in combination with nab­paclitaxel offers a promising treatment modality in different breast cancer subtypes.