RESUMO
In this study, we propose a generalized Marshall-Olkin exponentiated exponential distribution as a submodel of the family of generalized Marshall-Olkin distribution. Some statistical properties of the proposed distribution are examined such as moments, the moment-generating function, incomplete moment, and Lorenz and Bonferroni curves. We give five estimators for the unknown parameters of the proposed distribution based on maximum likelihood, least squares, weighted least squares, and the Anderson-Darling and Cramer-von Mises methods of estimation. To investigate the finite sample properties of the estimators, a comprehensive Monte Carlo simulation study is conducted for the models with three sets of randomly selected parameter values. Finally, four different real data applications are presented to demonstrate the usefulness of the proposed distribution in real life.
Assuntos
Simulação por Computador , Distribuições Estatísticas , Método de Monte Carlo , Análise dos Mínimos QuadradosRESUMO
Complex human diseases such as metabolic disorders, cancer, neurodegenerative diseases, and mitochondrial dysfunctions arise from the biochemical or genetic defects in various cellular processes. Therefore, it is important to understand which metabolic processes are affected by which cellular impairment. Because genome-wide screening of mutant collections (haploid/diploid deletion library) provides important clues for the understanding of conserved biological processes and for finding potential target genes, we screened the haploid mutant collection of Schizosaccharomyces pombe with wortmannin that inhibits phosphatidylinositol-3-kinase signaling. Using genome-wide screening, we determined that 52 mutants were resistant to this chemical. When 52 genes that are deleted in these mutants were grouped in 41 different biological processes, we found that 37 of them have human orthologues and 4 genes were associated with human metabolic disorders. In addition, when we examined the pathways in which these 52 genes function, we determined that 9 genes were related to phosphorylation process. These results might provide new insights for better understanding of certain human diseases.
Assuntos
Antifúngicos/farmacologia , Farmacorresistência Fúngica/genética , Estudo de Associação Genômica Ampla , Mutação , Proteínas de Schizosaccharomyces pombe/metabolismo , Schizosaccharomyces/genética , Wortmanina/farmacologia , Genoma Fúngico , Humanos , Fosforilação , Schizosaccharomyces/efeitos dos fármacos , Proteínas de Schizosaccharomyces pombe/genética , Transdução de SinaisRESUMO
This study focuses on the effect of iron on hexose transporters which perform glucose uptake. For this aim, we investigated the role of iron in glucose utilization and expression of hexose transporters in Schizosaccharomyces pombe. We applied different iron concentrations (1, 2, 5, 10 mM) to the cells grown up to mid-logarithmic phase. According to analysis of cell viability and morphology, we determined 2 mM and 5 mM as non-toxic and toxic doses, respectively. Besides, glucose consumption efficiency increased (1.5-fold) in the cells which were exposed to these iron concentrations. qRT-PCR analysis of hexose transporter genes showed that the expression of ght2 and ght8 genes were downregulated under both non-toxic and toxic iron conditions, but that of ght5 gene was significantly decreased only by toxic iron dose. In conclusion, it was suggested for the first time in this study that the Ght5 protein, as being high affinity hexose transporter, might play a role in sensing and signaling of iron stress.
