Evaluating the effect of microalga Haematococcus pluvialis bioaugmentation on aerobic membrane bioreactor in terms of performance, membrane fouling and microbial community structure.

In this study, considering the enhancement potential of microalgae and MBRs for wastewater treatment, the microalgae Haematococcus pluvialis, which is a freshwater species of Chlorophyta with a high capacity to synthesize astaxanthin, was bioaugmented into an aerobic MBR to investigate its potential on treatment of antibiotics in wastewater, reducing membrane biofouling, and impact on the microbial community structure. For this purpose, two control MBRs, with and without antibiotics, alongside an MBR bioaugmented with H. pluvialis, were set under mesophilic conditions, using inoculum from a local wastewater treatment facility and synthetic wastewater. The common antibiotics sulfamethoxazole (SMX), tetracycline (TET) and erythromycin (ERY) were selected to investigate removal efficiencies by Haematococcus pluvialis in an MBR for this study. In the bioaugmented reactor, membrane biofouling was delayed by 33% and chemical oxygen demand removal increased by 6%. The highest removal of antibiotics was observed for TET with a 20% enhancement from 69.75% (C2) to 89.73% (HP). The results also suggested that H. pluvialis reconstructed indigenous and biofilm microbial communities in MBR. The biodegradation network was modified and the relative abundance of Proteobacteria increased, while Firmicutes and Bacteroidetes were significantly reduced.

Addition of Trichocladium canadense to an anaerobic membrane bioreactor: evaluation of the microbial composition and reactor performance.

Membrane bioreactors are powerful systems for wastewater treatment and the removal of toxic compounds. However, membrane biofouling stands in the way of their widespread usage. In this study, the saprophytic fungus Trichocladium canadense was used as the bioaugmentor in an anaerobic membrane bioreactor (AnMBR) and its impact on membrane biofouling, biogas production, the microbial communities of the reactor and removal of the common antibiotics erythromycin (ERY), sulfamethoxazole (SMX) and tetracycline (TET) from synthetic wastewater was investigated. The results indicated that through bioaugmentation with 20% T. canadense, membrane biofouling was slowed by 25%, the chemical oxygen demand removal increased by 16% and a higher efficiency removal of ERY and SMX was achieved. The presence of T. canadense significantly increased the abundance and diversity of the biofilm archaeal community and the bacterial phylum Firmicutes, a known bio-foulant.