[Profiles of IgG responses against CSP, GLURP and LSA-3NR2 in urban malaria (Dakar): relations with haemoglobin levels and parasite densities]. / Profils des réponses IgG dirigées contre CSP, GLURP et LSA-3NR2 dans le paludisme urbain à Dakar : influence sur l'hémoglobinémie et les parasitémies circulantes.

Malaria remains a major health problem in sub- Saharan African countries despite substantial decreases in morbidity and mortality due to sustained control programs. Vaccines candidates were mainly tested in rural endemic setting; however increasing proportion of the population is living in urban area. Evaluation of the qualitative or quantitative immune responses to key targets of anti-Plasmodium immunity requires further investigation in urban area. In a cohort of 144 patients with mild malaria living in Dakar, we analyzed IgG responses against target antigens of P. falciparum: CSP, LSA-3NR2 and GLURP by ELISA. A mean age of 15 yrs (4-65 yrs) was found and patients were separated in 59 adults (<15yrs) and 85 children (≤15 yrs). Parasites densities (0,01-15%) did not differ between the two age groups. In contrast, haemoglobin levels appeared lower in children (4.5-16.6 g/dl) (p<0.01). For the immune results, the most recognized antigens were GLURP and CSP compared to LSA-3NR2. Levels of IgG against these antigens were significantly different between the two age groups and they were positively correlated (rho = 0.32; p<0.001). In addition, levels of IgG anti-GLURP were associated with low parasitemia (≤1%) and absence of anemia (≥11g/dl), particularly in adults (p<0.001). In a multiple regression analysis, no significant relationship was found between parasite densities and IgG responses against all the tested antigens. Our study shows the implication of IgG anti-GLURP in humoral immune response against the parasite. The present work contributes to determine IgG levels that can be used as relevant immunologic biomarkers in urban clinical malaria.

[Detection of anti-Lassa antibodies in the Western Forest area of the Ivory Coast]. / Detection des anticorps anti-virus de Lassa dans l'ouest forestier de la Côte d'Ivoire.

Lassa fever is an African viral hemorrhagic fever (VHF) known to be endemic in a number of West African countries including Nigeria, Sierra Leone, Liberia and Guinea. Despite having common borders with Liberia and Guinea, Côte d'Ivoire has never reported any cases of Lassa fever. In March 2000, as part of a research project on VHF--mainly yellow fever, Lassa fever and Ebola fever--in Guinea and Cote d'Ivoire, an exploratory survey was conducted to assess knowledge about VHF and immunological status against Lassa virus among forest workers in the Duekoue and Guiglo regions. One hundred and sixty-three male forest workers were interviewed using a questionnaire designed to assess risk factors for VHF exposure and personal medical history over the last 12 months. Detection of IgG antibodies against Lassa virus was performed by immunofluorescence assay with Lassa virus antigens from the Josiah and Las/AV strains. The overall prevalence of IgG antibodies was 26% (42/161). Among the Lassa IgG positive subjects, 38.5% were loggers including 20% that were positive at a serum dilution of 1/40 and 46.7% were national park workers or forest rangers including 69% that were positive at a dilution of 1/40 and more. Forty-one percent of subjects had heard of VHF including 14% who attributed it to animals and 2% who attributed it to plants. Contact with rodents was frequent and more than 50% of subjects had either eaten or skinned rodents. Although the prevalence of anti-Lassa IgG antibodies seemed high in the study population, no conclusion can be about level of exposure to Lassa virus.

Detection des anticorps anti-virus de Lassa dans l'ouest forestier de la Cote d'Ivoire

La fievre de Lassa est une fievre hemorragique virale (FHV) africaine endemique en Afrique de l'Ouest; notamment au Nigeria; en Sierra Leone; au Liberia et en Guinee. La Cote d'ivoire qui part age des frontieres avec le Liberia et la Guinee n'a jamais notifie de cas de fievre de Lassa. Dans le cadre d'un projet de recherche sur les fievres hemorragiques virales principalement la fievre jaune; la fievre de Lassa et la fievre Ebola en Guinee et en Cote d'Ivoire; une enquete a ete realisee en mars 2000 chez des travailleurs forestiers des prefectures de Guiglo et Duekoue; dans le but de determiner le niveau de connaissance sur les fievres hemorra-giques virales et le statut immunitaire vis a vis du virus de Lassa. Cent soixante-trois sujets de sexe masculin professionnels de la foret ont ete interroges a l'aide d'un questionnaire sur les facteurs de risque d'exposition aux FHV et leurs antecedents medicaux des 12 dern i e rs mois. Les anticorps seriques de type IgG anti-virus de Lassa ont ete detectes par technique d'immunofluorescence utilisant les antigenes Lassa des souches Josuah et LAS/AV. La prevalence globale en IgG obtenue etait de 26(42/161). Pa rmi les sujets positifs; 38;5etaient des bucherons dont 20positifs au 1/40; 46;7des agents du parc national ou gardes forestieres dont 69positifs au 1/40 et plus. De plus; 41des sujets avaient entendu parler des FHV; 14ont attribue leur origine a des animaux et 2a des plantes. Les contacts avec les rongeurs etaient frequents et plus de 50des personnes ont deja consomme ou depece des rongeurs. La proportion de sujets porteurs d'anticorps IgG anti-virus de Lassa dans cette population semblait elevee. Cependant cette prevalence ne permet pas de conclure a une exposition particuliere au virus de Lassa