RESUMO
The article deals with the results of studying the growth characteristics of nine nutrient mediums for primary plating of pathologic material. It is demonstrated that for successful and proper functioning of bacteriologic laboratory providing bacterial analysis for diphtheria the permanent quality control is needed to monitor the nutrient mediums for primary plating. The quality control is applied to evaluate the growth characteristics on such criteria as germination of isolated culture, intensity of its growth in 24 and 48 hours, optimal size of colonies characterized by their cultural characteristics, inhibiting activity concerning concurrent microflora.
Assuntos
Técnicas de Tipagem Bacteriana/normas , Corynebacterium diphtheriae , Meios de Cultura/normas , Difteria , Técnicas de Tipagem Bacteriana/métodos , Corynebacterium diphtheriae/classificação , Corynebacterium diphtheriae/crescimento & desenvolvimento , Corynebacterium diphtheriae/isolamento & purificação , Difteria/diagnóstico , Difteria/microbiologia , Feminino , Humanos , Masculino , Controle de QualidadeRESUMO
A salmonellosis outbreak, caused by S. typhimurium, was investigated with the use of some microbiological and molecular-biological methods of typing. This investigation revealed that the outbreak was caused by the "outbreak" electrotype of the multi-resistant variant of the infective agent, found to have several plasmidovars. The possibilities and limitations of typing by sensitivity to antibiotics and plasmid DNA profile were shown. These methods of intraspecific typing were regarded as methods making it possible to establish the heterogeneity of S. typhimurium with the use of intraclonal markers.
