RESUMO
Species determination of tissue specimens, including blood, is an important component of forensic analysis to distinguish human from animal remains. DNA markers based on a method of species-specific PCR and amplifying the 359-base pair (bp) fragment of the mitochondrially encoded cytochrome-b gene and then digestion with the TaqI restriction enzyme were developed for detection and discrimination of human, cattle, buffalo, horse, sheep, pig, dog, cat and chicken blood samples. The results reveal that PCR-amplification of the gene encoding the species-specific repeat (SSR) region generated 603 bp in cattle and buffalo, 221 bp in horse, 374 bp in sheep, Assuntos
Impressões Digitais de DNA/métodos
, Polimorfismo de Fragmento de Restrição
, Animais
, Búfalos/genética
, Gatos/genética
, Bovinos/genética
, Galinhas/genética
, Citocromos b/genética
, Primers do DNA
, DNA Mitocondrial/genética
, Cães/genética
, Eletroforese em Gel de Ágar
, Cavalos/genética
, Humanos
, Reação em Cadeia da Polimerase
, Ovinos/genética
, Especificidade da Espécie
, Suínos/genética
, Taq Polimerase/genética
