Expression of concern: Antibacterial and antibiofilm activities of silver-decorated zinc ferrite nanoparticles synthesized by a gamma irradiation-coupled sol-gel method against some pathogenic bacteria from medical operating room surfaces.

Expression of concern for 'Antibacterial and antibiofilm activities of silver-decorated zinc ferrite nanoparticles synthesized by a gamma irradiation-coupled sol-gel method against some pathogenic bacteria from medical operating room surfaces' by M. I. A. Abdel Maksoud et al., RSC Adv., 2021, 11, 28361-28374, https://doi.org/10.1039/D1RA04785J.

Evaluation of barium tungstate nanocrystals for the sorption of radioactive cobalt and europium from aqueous solutions.

Herein, barium tungstate BaWO4 nanocrystals were chemically prepared and then estimated as inorganic sorbent material to eliminate the radioactive cobalt and europium from the waste stream. The characterization of BaWO4 nanocrystals is completed over several analytical techniques. TEM and SEM images show the formation of sphere-shaped BaWO4 structures in the nanoscale range. Also, XRD and FTIR revealed the successful preparation of BaWO4. Optimum factors affected by the sorption process are determined using batch mode. Sorption equilibrium was achieved after 60 min with the initial concentration of metal ion at 100 mg/L and at optimum pH five for both radionuclides, respectively. The different kinetic models are applied. The obtained data shows that the sorption process followed a pseudo-second order. The sorption capacity for 60Co at pH of 5 and 25 °C is 310.6 mg g-1, and 152+154Eu is 409.9 mg g-1. The thermodynamic studies illustrated that the sorption process was spontaneous and endothermic.

Utilization of carbon-coated ZrO2/Mn-Mg-Zn ferrites nanostructures for the adsorption of Cs (I) and Sr (II) from the binary system: kinetic and equilibrium studies.

Carbon-coated ZrO2/Mn-Mg-Zn ferrites nanostructures (CZ-FN) have been prepared as a new inorganic sorbent to remove Cs (I) and Sr (II) from a waste stream. Adsorption of Cs (I) and Sr (II) has been implemented considering different noteworthy parameters, for example, shaking time and the optimum time achieved high adsorption capacity of both ions [103 and 41 mg/g for Sr (II) and Cs (I)] was found 30 min. Also, the impact of pH values was studied; the best pH value for the adsorption process is pH 6. The adsorption saturation capacity of CZ-FN is 420.22 and 250.45 mg/g for strontium and cesium, respectively. The solubility percentage of CZ-FN was calculated utilizing diverse molarities from HNO3, HCl, and NaOH as eluents, the obtained data reveals an increase in the solubility percentage with more increase in the molarity of the eluents. The elevation in the solubility percentage follows the following order; HNO3 < HCl < NaOH. The kinetic studies were applied using the nanolinear form of different kinetic models; it was found that the adsorption process obeys the nonlinear pseudo-second-order. According to equilibrium studies, the Langmuir model has been more accurate than the Freundlich model for adsorption in the case of binary systems. The values of Di for the strontium and cesium are 10-10 m2/s, which displays the chemisorption nature of this process. The greatest values of the desorption process for the strontium and cesium are 96.87% and 94.43 by 0.3 M of HNO3. This indicated that the carbon-coated ZrO2/Mn-Mg-Zn ferrites could be regenerated and recycled to remove strontium and cesium ions from waste streams.

Recycled high-density polyethylene plastic reinforced with ilmenite as a sustainable radiation shielding material.

In this work, recycled high-density polyethylene plastic (r-HDPE) reinforced with ilmenite mineral (Ilm) in different ratios (0, 15, 30, and 45 wt%) as a sustainable and flexible radiation shielding material was manufactured using the melt blending method. XRD patterns and FTIR spectra demonstrated that the polymer composite sheets were successfully developed. The morphology and elemental composition were addressed using SEM images and EDX spectra. Moreover, the mechanical characteristics of the prepared sheets were also studied. The gamma-ray attenuation characteristics for established r-HDPE + x% Ilm composite sheets were theoretically computed between 0.015 and 15 MeV using Phy-X/PSD software. Also, the mass attenuation coefficients have been compared to their values by the WinXCOM program. It is also shown that the shielding performance of the r-HDPE + 45% Ilm composite sheet is significantly greater than that of r-HDPE. As a result, the ilmenite-incorporated recycled high-density polyethylene sheets are suited for medical and industrial radiation shielding applications.

Gamma radiation-induced synthesis of novel PVA/Ag/CaTiO3 nanocomposite film for flexible optoelectronics.

A flexible nanocomposite film based on polyvinyl alcohol (PVA), silver nanoparticles, and calcium titanate (CaTiO3) was synthesized using gamma radiation induced-reduction. Temperature-dependent structural, optical, DC electrical conductivity, electric modulus, and dielectric properties of PVA/Ag/CaTiO3 nanocomposite film were investigated. The XRD pattern proved the successful preparation of the nanocomposite film. Also, as the temperature increases, the average crystallite sizes of CaTiO3 and Ag nanoparticles decrease from 19.8 to 9.7 nm and 25 to 14.8 nm, respectively. Further, the optical band gap increased from 5.75 to 5.84 eV with increasing temperature. The thermal stability is improved, and the semiconductor behavior for PVA/Ag/CaTiO3 nanocomposite film is confirmed by thermal activation energy ΔE with values in the 0.11-0.8 eV range. Furthermore, the maximum barrier Wm value was found of 0.29 eV. PVA/Ag/CaTiO3 nanocomposite film exhibits a semicircular arc originating from the material's grain boundary contributions for all temperatures. The optical, DC electrical conductivity, and dielectric properties of the PVA/Ag/CaTiO3 nanocomposite film can be suitable for flexible electronic devices such as electronic chips, optoelectronics, and energy storage applications.

Optical and radiation shielding properties of PVC/BiVO4 nanocomposite.

This study investigates the physical and optical properties as well as the radiation shielding capacity of polyvinyl chloride (PVC) loaded with x% of bismuth vanadate (BiVO4) (x = 0, 1, 3, and 6 wt%). As a non-toxic nanofiller, the designed materials are low-cost, flexible, and lightweight plastic to replace traditional lead, which is toxic and dense. XRD patterns and FTIR spectra demonstrated a successful fabrication and complexation of nanocomposite films. In addition, the particle size, morphology, and elemental composition of the BiVO4 nanofiller were demonstrated through the utilization of TEM, SEM, and EDX spectra. The MCNP5 simulation code assessed the gamma-ray shielding effectiveness of four PVC + x% BiVO4 nanocomposites. The obtained mass attenuation coefficient data of the developed nanocomposites were comparable to the theoretical calculation performed with Phy-X/PSD software. Moreover, the initial stage in the computation of various shielding parameters, such as half-value layer, tenth value layer, and mean free path, besides the simulation of linear attenuation coefficient. The transmission factor declines while radiation protection efficiency increases with an increase in the proportion of BiVO4 nanofiller. Further, the current investigation seeks to evaluate the thickness equivalent (Xeq), effective atomic number (Zeff), and effective electron density (Neff) values as a function of the concentration of BiVO4 in a PVC matrix. The results obtained from the parameters indicate that incorporating BiVO4 into PVC can be an effective strategy for developing sustainable and lead-free polymer nanocomposites, with potential uses in radiation shielding applications.

Adsorption and separation of Cs(I) and Ba(II) from aqueous solution using zinc ferrite-humic acid nanocomposite.

Reclaimable adsorbents have an essential role in removing radionuclides from waste streams. Herein, zinc ferrite-humic acid ZFO/HA nanocomposite was synthesized for effective cesium and barium adsorption. The prepared ZFO/HA nanocomposite was analyzed using analytical techniques including XRD, FTIR, EDX, and SEM. From kinetic studies, the mechanism adsorption process follows the second model. The isotherm studies clarified that the Langmuir model fit the adsorption of both ions onto the prepared sample, and the monolayer capacities are equal to 63.33 mg/g and 42.55 mg/g for Ba(II) and Cs(I), respectively. The temperature parameter was also studied, and the adsorption reaction was spontaneous and endothermic. The maximum separation between two ions was achieved at pH 5 (αCs/Ba = 3.3).

Cocatalyst loaded Al-SrTiO3 cubes for Congo red dye photo-degradation under wide range of light.

The continued pollution, waste, and unequal distribution of the limited amount of fresh water on earth are pushing the world into water scarcity crisis. Consequently, development of revolutionary, cost-effective, and efficient techniques for water purification is essential. Herein, molten flux method was used for the preparation of micro-sized Al-doped SrTiO3 photocatalyst loaded with RhCr2O3 and CoOOH cocatalysts via simple impregnation method for the photo-assisted degradation of Congo red dye under UV and visible irradiation compared with P25 standard photocatalyst. In addition, photoelectrochemical analysis was conducted to reveal the separation and transfer efficiency of the photogenerated e-/h+ pairs playing the key role in photocatalysis. SEM and TEM analyses revealed that both P25 and the pristine SrTiO3 have spherical shapes, while Al-doped SrTiO3 and the sample loaded with cocatalysts have cubic shapes with a relatively higher particle size reaching 145 nm. In addition, the lowest bandgap is due to Al+3 ion doping and excessive surface oxygen vacancies, as confirmed by both UV-Vis diffuse-reflectance and XPS analyses. The loading of the cocatalysts resulted in a change in the bandgap from n-type (pristine SrTiO3 and Al-SrTiO3) into p-type (cocatalyst loaded sample) as exhibited by Mott-Schottky plots. Besides, the cocatalyst-loaded sample exhibited good performance stability after 5 cycles of the photocatalytic removal of Congo red dye. OH· radical was the primary species responsible for CR degradation as confirmed by experiments with radical scavengers. The observed performance of the prepared samples under both UV and visible light could foster the ongoing efforts towards more efficient photocatalysts for water purification.

Enhanced photocatalytic and antimicrobial performance of a multifunctional Cu-loaded nanocomposite under UV light: theoretical and experimental study.

Due to modern industrialization and population growth, access to clean water has become a global challenge. In this study, a metal-semiconductor heterojunction was constructed between Cu NPs and the Co0.5Ni0.5Fe2O4/SiO2/TiO2 composite matrix for the photodegradation of potassium permanganate, hexavalent chromium Cr(VI) and p-nitroaniline (pNA) under UV light. In addition, the electronic and adsorption properties after Cu loading were evaluated using density functional theory (DFT) calculations. Moreover, the antimicrobial properties of the prepared samples toward pathogenic bacteria and unicellular fungi were investigated. Photocatalytic measurements show the outstanding efficiency of the Cu-loaded nanocomposite compared to that of bare Cu NPs and the composite matrix. Degradation efficiencies of 44% after 80 min, 100% after 60 min, and 65% after 90 min were obtained against potassium permanganate, Cr(VI), and pNA, respectively. Similarly, the antimicrobial evaluation showed high ZOI, lower MIC, higher protein leakage amount, and cell lysis of nearly all microbes treated with the Cu-loaded nanocomposite.

Gamma radiation-induced synthesis of a novel chitosan/silver/Mn-Mg ferrite nanocomposite and its impact on cadmium accumulation and translocation in brassica plant growth.

Herein, a novel chitosan/silver/Mn0.5Mg0.5Fe2O4 (Cs/Ag/MnMgFe2O4) nanocomposite was synthesized with gamma irradiation assistant. The prepared Cs/Ag/MnMgFe2O4 nanocomposite was characterized via EDX, XRD, SEM, UV-vis spectroscopy. To evaluate the effects of soak low and high-dose nanocomposite on physiological parameters, photosynthetic pigments, antioxidant and non-antioxidant enzymes of cabbage under Cd stress, a factorial experiment was conducted based on CRD with five replications. The Cd stress decreased the morphological characteristics and photosynthetic pigments while increasing cabbage's antioxidant and non-antioxidant enzymes. The application of low and high-dose of nanocomposite decreased Cd content in leaves by about 42.86%, 60.48%, and the root by approximately 18.72%, 28.72%, respectively, and translocation factors and tolerance index, H2O2, O2, and malondialdehyde. In contrast, the application of high of the nanocomposite increased the values of SPAD chlorophyll about 27.50%, stomatal conductance about 87.18%, net photosynthetic rate about 44.90%, intercellular CO2 concentration about 32.00%, and transpiration rate about 85.20%, as compared to Cd stress. Furthermore, the application of low and high-dose Cs/Ag/MnMgFe2O4 nanocomposite enhances the antioxidant and non-antioxidant enzymes of the cabbage plant compared to Cd stress. Generally, it was conducted that Cs/Ag/MnMgFe2O4 nanocomposite can be used as a proper tool for increasing cabbage plants under Cd stress.

Novel adsorbent based on carbon-modified zirconia/spinel ferrite nanostructures: Evaluation for the removal of cobalt and europium radionuclides from aqueous solutions.

Herein, a novel adsorbent based on carbon-modified zirconia/spinel ferrite (C@ ZrO2/Mn0.5Mg0.25Zn0.25Fe2O4) nanostructures were chemically prepared to remove 60Co and 152+154Eu radionuclides from liquid media using batch experiments. The XRD pattern confirms the successful preparation of the C@ZrO2/MnMgZnFe2O4 composite. Also, SEM and TEM images confirmed that the composite owns a heterogeneous morphology in the nanoscale range. The optical band gap value of Mn0.5Mg0.25Zn0.25Fe2O4, ZrO2, and the composite samples was 1.45, 2.38, and 1.54 eV, respectively. Many parameters have been studied as the effect of time, solution pH, and initial ion concentration. The kinetics models for the removal process of 152+154Eu and 60Co radionuclides were studied. The second-order kinetic equation could describe the sorption kinetics for both radionuclides. The Langmuir monolayer capacity for 60Co was 82.51 mg/g and for 152+154Eu was 136.98 mg/g. The thermodynamic parameters such as free energy ΔGo, the enthalpy ΔHo, and the entropy ΔSo were calculated. The results indicated that the sorption process has endothermic nature for both two radionuclides onto C@ZrO2/MnMgZnFe2O4 composite.

Influence of Ce3+ Substitution on Antimicrobial and Antibiofilm Properties of ZnCexFe2-xO4 Nanoparticles (X = 0.0, 0.02, 0.04, 0.06, and 0.08) Conjugated with Ebselen and Its Role Subsidised with γ-Radiation in Mitigating Human TNBC and Colorectal Adenocarcinoma Proliferation In Vitro.

Cancers are a major challenge to health worldwide. Spinel ferrites have attracted attention due to their broad theranostic applications. This study aimed at investigating the antimicrobial, antibiofilm, and anticancer activities of ebselen (Eb) and cerium-nanoparticles (Ce-NPs) in the form of ZnCexFe2-XO4 on human breast and colon cancer cell lines. Bioassays of the cytotoxic concentrations of Eb and ZnCexFe2-XO4, oxidative stress and inflammatory milieu, autophagy, apoptosis, related signalling effectors, the distribution of cells through the cell-cycle phases, and the percentage of cells with apoptosis were evaluated in cancer cell lines. Additionally, the antimicrobial and antibiofilm potential have been investigated against different pathogenic microbes. The ZOI, and MIC results indicated that ZnCexFe2-XO4; X = 0.06 specimen reduced the activity of a wide range of bacteria and unicellular fungi at low concentration including P. aeruginosa (9.5 mm; 6.250 µg/mL), S. aureus (13.2 mm; 0.390 µg/mL), and Candida albicans (13.5 mm; 0.195 µg/mL). Reaction mechanism determination indicated that after ZnCexFe2-xO4; X = 0.06 treatment, morphological differences in S.aureus were apparent with complete lysis of bacterial cells, a concomitant decrease in the viable number, and the growth of biofilm was inhibited. The combination of Eb with ZFO or ZnCexFe2-XO4 with γ-radiation exposure showed marked anti-proliferative efficacy in both cell lines, through modulating the oxidant/antioxidant machinery imbalance, restoring the fine-tuning of redox status, and promoting an anti-inflammatory milieu to prevent cancer progression, which may be a valuable therapeutic approach to cancer therapy and as a promising antimicrobial agent to reduce the pathogenic potential of the invading microbes.

Antibacterial and antibiofilm activities of silver-decorated zinc ferrite nanoparticles synthesized by a gamma irradiation-coupled sol-gel method against some pathogenic bacteria from medical operating room surfaces.

This work aimed at the gamma irradiation-assisted synthesis of silver (Ag)-decorated ZnFe2O4 (ZFO) ferrite nanoparticles (NPs), which were tested for their antibacterial and antibiofilm activities against some pathogenic bacteria from medical operating room surfaces. The prepared Ag-decorated ZFO NPs were characterized via XRD, SEM, EDX, elemental mapping, and FTIR analysis. The antibacterial potential was tested as ZOI and MIC, while antibiofilm activity was estimated by the tube method. The growth curve assay, the effect of UV on the antimicrobial activity, and cell membrane leakage were evaluated, and the antibacterial reaction mechanism was investigated by SEM/EDX analysis. The XRD and FTIR results confirmed the successful preparation of Ag-decorated ZFO NPs. Antibacterial results revealed that the most potent decorated sample was Ag0.75@ZFO NPs, recording the most significant inhibition zone against Staphylococcus vitulinus (24.67 ± 0.577 mm) and low MIC (0.097 µg mL-1) against S. vitulinus. The antibiofilm activity of Ag0.75@ZFO NPs was the highest, recorded as 97.3% for S. aureus and 95.25% for Enterococcus columbae. In the case of UV exposure, bacterial growth reached the lowest grade. Finally, it was seen that the amount of cellular protein released from bacterial cells is directly proportional to the concentration of Ag0.75@ZFO NPs, which clearly explains the formation of pits in the cell membrane. The synthesized nanocomposites may find an application after mixing with operating room paints to reduce the harmful effect of pathogenic microbes and, therefore, eliminate bacterial contamination.

Nanocomposite matrix conjugated with carbon nanomaterials for photocatalytic wastewater treatment.

The problem of hazardous wastewater remediation is a complicated issue and a global challenge. Herein, a layered Co0.5Ni0.5Fe2O4/SiO2/TiO2 composite matrix was prepared and incorporated with three carbon nanomaterials having different dimensionalities, carbon dots (C-dots, 0D), single-walled carbon nanotubes (1D), and reduced graphene oxide (2D), in an effort to create effective photocatalytic nanocomposites for chloramine-T removal from water. Microstructural analyses confirmed the formation of nanocomposites and revealed their chemistry and structure. Elemental mapping revealed a uniform distribution of elements throughout the nanocomposite matrix that was free of impurities. The spherical shape of the matrix particles (average diameter ~90 nm) and their conjugation with the carbon nanomaterials were confirmed. Nitrogen adsorption-desorption isotherms revealed that the nanocomposites were mesoporous but also contained macropores. The surface chemical compositions of the nanocomposites were investigated and showed a range of available binding energies. The kinetics of photocatalysis by the system were studied, and the effects of different parameters (such as photocatalyst dose and charge-carrier scavengers) on the efficiency of chloramine-T degradation were also investigated. The nanocomposite loaded with 10% C-dots exhibited high UV-assisted photocatalytic activity for chloramine-T degradation (65% removal efficiency).

Nanostructured Mg substituted Mn-Zn ferrites: A magnetic recyclable catalyst for outstanding photocatalytic and antimicrobial potentials.

With recently increasing the environmental problems and expected energy crisis, it is necessary to synthesis a low-cost, efficient, and UV-light responsive photocatalyst for contaminants' degradation. The nanostructured spinel ferrite Mn0.5Zn0.5-xMgxFe2O4 NPs (x = 0.0, 0.125, 0.25, 0.375 and 0.50) were synthesized via the sol-gel method. The crystallite size was lied in nano regime ranging from 21.8 to 36.5 nm. The surface chemical composition of the Mn0.5Zn0.5-xMgxFe2O4 NPs was investigated via XPS analysis. Mossbauer spectra showed that the peaks were shifted to higher values of the maximum magnetic field as the Mg content increased, indicating that the crystallinity is enhanced while the crystal size is decreased. Also, various parameters such as the photocatalyst dose, dyes concentration, pH, point of zero charge, and the metals leaching were studied. The point of zero charge (PZC) has found at pH = 2.38. The Mn0.5Zn0.125Mg0.375Fe2O4 NPs showed an excellent UV-assisted photocatalytic activity against Chloramine T (90 % removal efficiency) and Rhodamine B (95 % removal efficiency) after 80 min as compared to pure Mn0.5Zn0.5Fe2O4 ferrite NPs. Besides, it a recyclable catalyst at least four times with a negligible reduction of photocatalytic activity with slight elements leaching. Furthermore, the Mn0.5Zn0.25Mg0.25Fe2O4 NPs showed a high antimicrobial activity towards pathogenic bacteria and yeats.

Fabrication of Ultra-Pure Anisotropic Zinc Oxide Nanoparticles via Simple and Cost-Effective Route: Implications for UTI and EAC Medications.

The purposes of this work are to evaluate the antimicrobial, antibiofilm, anticancer, and antioxidant abilities of anisotropic zinc oxide nanoparticles (ZnO NPs) synthesized by a cost-effective and eco-friendly sol-gel method. The synthesized ZnO NPs were entirely characterized by UV-Vis, XRD, FTIR, HRTEM, zeta potential, SEM mapping, BET surface analyzer, and EDX elemental analysis. Antimicrobial and antibiofilm activities of ZnO NPs were investigated against multidrug-resistant (MDR) bacteria and yeast causing serious diseases like urinary tract infection (UTI). The anticancer activity was performed against Ehrlich ascites carcinoma (EAC). Additionally, antioxidant scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) was observed. The synthesized ZnO NPs exhibited an absorption peak at 385.0 nm characteristic to the surface plasmon resonance (SPR). Data obtained from HRTEM, SEM, and XRD confirmed the anisotropic crystalline nature of the prepared ZnO NPs with an average particle size of 68.2 nm. The calculated surface area of the prepared ZnO NPs was 10.62 m2/g and the porosity was 13.16%, while pore volume was calculated to be 0.013 cm3/g and the average pore size was about 3.10 nm. The prepared ZnO NPs showed promising antimicrobial activity against all tested UTI-causing pathogens. It showed a prominent antimicrobial capability against Candida tropicalis with a zone of inhibition (ZOI) reaching 22.4 mm, 13 mm ZOI for Bacillus subtilis, and 12.5 mm ZOI for Pseudomonas aeruginosa. Additionally, the prepared ZnO NPs showed enhanced biofilm repression of about 79.33%, 72.94%, and 33.68% against B. subtilis, C. tropicalis, and P. aeruginosa, respectively. Moreover, the prepared ZnO NPs had a powerful antioxidant property with 33.0% scavenging ability after applied DPPH assay. Surprisingly, upon ZnO NPs treatment, cancer cell viability reduced from 100 to 58.5% after only 24 h due to their unique antitumor activity. Therefore, according to these outstanding properties, this study could give insights for solving serious industrial, pharmaceutical, and medical challenges, particularly in the EAC and UTI medications.

1,2-Dihydroxyanthraquinone: Synthesis, and induced changes in the structural and optical properties of the nanostructured thin films due to γ-irradiation.

1,2­Dihydroxyanthraquinone (Alizarin-AZ) is available, low-cost organic compound. Besides, AZ has multiple applications owing to its drawing attention photoactivity. This paper is devoted to study the influence of Gamma irradiation on the morphology, optical, and dielectric properties of AZ nanostructured thin films. Nanostructure powder of Alizarin is synthesized according to chemical routes. Subsequently, thin films of AZ are fabricated via thermal evaporator. The bared thin film is irradiated at different doses of 60Co γ-rays. Furthermore, the bared and irradiated films are characterized via X-ray diffraction (XRD), atomic force microscope (AFM) and UV-Vis-NIR spectroscopy. XRD investigations reveal that the bared film has a nanostructure and the average particle size increases gradually as the γ-irradiation dose increases. AFM images show remarkable increment in the surface roughness of the irradiated film over the bared one. In the light of structure induced changes, clear variations in the optical properties are addressed. Of these, the energy gap decreases gradually as the irradiation dose increases. The film irradiated at 45 kGy shows the highest optical conductivity. Based on our results we suggest AZ nanostructured thin films as potential candidate for optoelectronics devices.

Synthesis and characterization of metals-substituted cobalt ferrite [Mx Co(1-x) Fe2O4; (M = Zn, Cu and Mn; x = 0 and 0.5)] nanoparticles as antimicrobial agents and sensors for Anagrelide determination in biological samples.

Nanocrystalline spinel ferrite nanoparticles [MxCo(1-x)Fe2O4;(M = Zn,Cu,Mn;x = 0 and 0.5)] like: Cobalt ferrite (CFO), Zinc Cobalt ferrite (ZCFO), Copper Cobalt ferrite (CCFO), and Manganese Cobalt ferrite (MCFO) modified carbon paste electrodes (CPE) were synthesized via sol-gel technique utilizing citric acid and ethylene glycol as a polymerization agent. The synthesized ferrite NPs were used as bi-functional smart biosensor, not only used to determine the drug Anagrelide-HCl (ANDH) in urine and serum samples, but also possesses antimicrobial potential against some pathogenic microbes, founded in the biological samples. The synthesized ferrite NPs were confirmed by XRD, FTIR spectroscopy, SEM, EDX, and elemental mapping images. Antimicrobial activities of ferrite NPs against selected urinary tract infected microbes were investigated. From XRD data and FTIR spectroscopy it is found that the average crystallite size is lies in the range 12.86 to 33.92 ±â€¯1.5 nm, also the bond lengths RA and RB increase from 1.8986 to 1.9145 Šand from 2.0434 to 2.0606 Šrespectively and Debye temperature θD lies in the range of 681.52-708.87 K. Our study describes the improvement of a screen-printed sensor, modified with ferrite NPs materials for rapid, sensitive and cost-effective quantification of ANDH present in the real samples such as blood serum samples, urine and in the pharmaceutical formulations. The results obtained postulate a linear regression between the ANDH charge density of peak current and its concentration in the range from (0.64-8.18 µg/ml) with DL 0.31 µg/ml and QL 0.94 µg/ml. Antimicrobial results indicated that ZCFO NPs were a novel antibacterial agent against Klebsiella pneumoniae (28.0 mm ZOI), and multidrug-resistant bacteria Enterococcus faecalis (27.0 mm ZOI). Additionally, ZCFO NPs were active against Candida albicans (18.0 mm ZOI) seems to be a smart antifungal agent. Therefore, ZCFO NPs can be used as applicant resources for industrial, medical, and biological applications.