Surface-coated magnetic nanostructured materials for robust bio-catalysis and biomedical applications-A review.

Background: Enzymes based bio-catalysis has wide range of applications in various chemical and biological processes. Thus, the process of enzymes immobilization on suitable support to obtain highly active and stable bio-catalysts has great potential in industrial applications. Particularly, surface-modified magnetic nanomaterials have garnered a special interest as versatile platforms for biomolecules/enzyme immobilization. Aim of review: This review spotlights recent progress in the immobilization of various enzymes onto surface-coated multifunctional magnetic nanostructured materials and their derived nano-constructs for multiple applications. Conclusive remarks, technical challenges, and insightful opinions on this field of research which are helpful to expand the application prospects of these materials are also given with suitable examples. Key scientific concepts of review: Nanostructured materials, including surface-coated magnetic nanoparticles have recently gained immense significance as suitable support materials for enzyme immobilization, due to their large surface area, unique functionalities, and high chemical and mechanical stability. Besides, magnetic nanoparticles are less expensive and offers great potential in industrial applications due to their easy recovery and separation form their enzyme conjugates with an external magnetic field. Magnetic nanoparticles based biocatalytic systems offer a wide-working temperature, pH range, increased storage and thermal stabilities. So far, several studies have documented the application of a variety of surface modification and functionalization techniques to circumvent the aggregation and oxidation of magnetic nanoparticles. Surface engineering of magnetic nanoparticles (MNPs) helps to improve the dispersion stability, enhance mechanical and physicochemical properties, upgrade the surface activity and also increases enzyme immobilization capabilities and biocompatibility of the materials. However, several challenges still need to be addressed, such as controlled synthesis of MNPs and clinical aspects of these materials require consistent research from multidisciplinary scientists to realize its practical applications.

Dilated Semantic Segmentation for Breast Ultrasonic Lesion Detection Using Parallel Feature Fusion.

Breast cancer is becoming more dangerous by the day. The death rate in developing countries is rapidly increasing. As a result, early detection of breast cancer is critical, leading to a lower death rate. Several researchers have worked on breast cancer segmentation and classification using various imaging modalities. The ultrasonic imaging modality is one of the most cost-effective imaging techniques, with a higher sensitivity for diagnosis. The proposed study segments ultrasonic breast lesion images using a Dilated Semantic Segmentation Network (Di-CNN) combined with a morphological erosion operation. For feature extraction, we used the deep neural network DenseNet201 with transfer learning. We propose a 24-layer CNN that uses transfer learning-based feature extraction to further validate and ensure the enriched features with target intensity. To classify the nodules, the feature vectors obtained from DenseNet201 and the 24-layer CNN were fused using parallel fusion. The proposed methods were evaluated using a 10-fold cross-validation on various vector combinations. The accuracy of CNN-activated feature vectors and DenseNet201-activated feature vectors combined with the Support Vector Machine (SVM) classifier was 90.11 percent and 98.45 percent, respectively. With 98.9 percent accuracy, the fused version of the feature vector with SVM outperformed other algorithms. When compared to recent algorithms, the proposed algorithm achieves a better breast cancer diagnosis rate.

Impact of Macrodiols on the Morphological Behavior of H12MDI/HDO-Based Polyurethane Elastomer.

In this study, we evaluated the morphological behavior of polyurethane elastomers (PUEs) by modifying the soft segment chain length. This was achieved by increasing the soft segment molecular weight (Mn = 400-4000 gmol-1). In this regard, polycaprolactone diol (PCL) was selected as the soft segment, and 4,4'-cyclohexamethylene diisocyanate (H12MDI) and 1,6-hexanediol (HDO) were chosen as the hard segments. The films were prepared by curing polymer on Teflon surfaces. Fourier transform infrared spectroscopy (FTIR) was utilized for functional group identification in the prepared elastomers. FTIR peaks indicated the disappearance of -NCO and -OH groups and the formation of urethane (NHCOO) groups. The morphological behavior of the synthesized polymer samples was also elucidated using scanning electron microscopy (SEM) and atomic force microscopy (AFM) techniques. The AFM and SEM results indicated that the extent of microphase separation was enhanced by an increase in the molecular weight of PCL. The phase separation and degree of crystallinity of the soft and hard segments were described using X-ray diffraction (XRD). It was observed that the degree of crystallinity of the synthesized polymers increased with an increase in the soft segment's chain length. To evaluate hydrophilicity/hydrophobicity, the contact angle was measured. A gradual increase in the contact angle with distilled water and diiodomethane (38.6°-54.9°) test liquids was observed. Moreover, the decrease in surface energy (46.95-24.45 mN/m) was also found to be inconsistent by increasing the molecular weight of polyols.

Mn3O4 nanoparticles: Synthesis, characterization and their antimicrobial and anticancer activity against A549 and MCF-7 cell lines.

Due to their inexpensive and eco-friendly nature, and existence of manganese in various oxidation states and their natural abundance have attained significant attention for the formation of Mn3O4 nanoparticles (Mn3O4 NPs). Herein, we report the preparation of Mn3O4 nanoparticles using manganese nitrate as a precursor material by utilization of a precipitation technique. The as-prepared Mn3O4 nanoparticles (Mn3O4 NPs) were characterized by using X-ray powder diffraction (XRD), UV-Visible spectroscopy (UV-Vis), High-Resolution Transmission electron microscopy (HRTEM), Field emission scanning electron microscopy (FESEM), Thermal gravimetric analysis (TGA) and Fourier-transform infrared spectroscopy (FT-IR). The antimicrobial properties of the as-synthesized Mn3O4 nanoparticles were investigated against numerous bacterial and fungal strains including S. aureus, E. coli, B. subtilis, P. aeruginosa, A. flavus and C. albicans. The Mn3O4 NPs inhibited the growth of S. aureus with a minimum inhibitory concentration (MIC) of 40 µg/ml and C. albicans with a MIC of 15 µg/ml. Furthermore, the Mn3O4 NPs anti-cancer activity was examined using MTT essay against A549 lung and MCF-7 breast cancer cell lines. The Mn3O4 NPs revealed significant activity against the examined cancer cell lines A549 and MCF-7. The IC50 values of Mn3O4 NPs with A549 cell line was found at concentration of 98 µg/mL and MCF-7 cell line was found at concentration of 25 µg/mL.

Influence of Shopping Bags Carrying on Human Responses While Walking.

Shopping as a daily activity that involves carrying shopping bags in hands might be associated with risk factors contributing to the development of low back pain (LBP) and strains and sprains in the upper extremity. A three-way repeated measures experiment was conducted for the purpose of the study. The independent variables were holding style, carrying technique, and shopping bags' weights. The dependent variables were cardiac cost, muscles' activities as a percentage of their maximum voluntary contraction's EMG (%MVC), peak plantar pressure (PPP), and discomfort rating. Carrying grocery bags with both hands to the sides of the body using shopping bags' holder was favorable and advantageous to other carrying conditions in terms of less cardiac cost, less %MVC, less peak plantar pressure, and less discomfort. It is useful to carry grocery bags close to the body with both hands using holders that are available in the local market.