Effect of Pseudomonas fluorescens and pyoverdine on the phytoextraction of cesium by red clover in soil pots and hydroponics.

With the aim of improving the phytoextraction rate of cesium (Cs), the effect of Pseudomonas fluorescens ATCC 17400 and its siderophore pyoverdine (PVD) on the uptake of Cs by red clover was studied in soil pots. This work also provides a mechanistic understanding of the Cs-bacteria (or PVD)-illite-plant interactions by using a simplified experimental design, i.e., hydroponics with either Cs in solution or Cs-spiked illite in suspension. For soil spiked with 11.2 mmol kg-1 (1480 mg kg-1) of Cs, 0.43% of total Cs was taken up by red clover in 12 days (119 µmol g-1 (16 mg g-1) of Cs dry matter in roots and 40 µmol g-1 (5 mg g-1) in shoots). In hydroponics with Cs in solution (0.1 mmol L-1 or 13 mg L-1), 75% of Cs was taken up vs. only 0.86% with Cs-spiked illite suspension. P. fluorescens and PVD did not increase Cs concentrations in aboveground parts and roots of red clover and even decreased them. The damaging effect of PVD on red clover growth was demonstrated with the biomass yielding 66% of the control in soil pots (and 100% mortality after 12 days of exposition) and only 56% in hydroponics (78% with illite in suspension). Nonetheless, PVD and, to a lesser extent, P. fluorescens increased the translocation factor up to a factor of 2.8. This study clearly showed a direct damaging effect of PVD and to a lower extent the retention of Cs by biofilm covering both the roots and illite, both resulting in the lower phytoextraction efficiency.

Chloride and organic chlorine in forest soils: storage, residence times, and influence of ecological conditions.

Recent studies have shown that extensive chlorination of natural organic matter significantly affects chlorine (Cl) residence time in soils. This natural biogeochemical process must be considered when developing the conceptual models used as the basis for safety assessments regarding the potential health impacts of 36-chlorine released from present and planned radioactive waste disposal facilities. In this study, we surveyed 51 French forested areas to determine the variability in chlorine speciation and storage in soils. Concentrations of total chlorine (Cl(tot)) and organic chlorine (Cl(org)) were determined in litterfall, forest floor and mineral soil samples. Cl(org) constituted 11-100% of Cl(tot), with the highest concentrations being found in the humus layer (34-689 mg Cl(org) kg(-1)). In terms of areal storage (53 - 400 kg Cl(org) ha(-1)) the mineral soil dominated due to its greater thickness (40 cm). Cl(org) concentrations and estimated retention of organochlorine in the humus layer were correlated with Cl input, total Cl concentration, organic carbon content, soil pH and the dominant tree species. Cl(org) concentration in mineral soil was not significantly influenced by the studied environmental factors, however increasing Cl:C ratios with depth could indicate selective preservation of chlorinated organic molecules. Litterfall contributions of Cl were significant but generally minor compared to other fluxes and stocks. Assuming steady-state conditions, known annual wet deposition and measured inventories in soil, the theoretical average residence time calculated for total chlorine (inorganic (Cl(in)) and organic) was 5-fold higher than that estimated for Cl(in) alone. Consideration of the Cl(org) pool is therefore clearly important in studies of overall Cl cycling in terrestrial ecosystems.

The effect of high power ultrasound on an aqueous suspension of graphite.

Ultrasound treatment was used to study the decrease of the granulometry of graphite, due to the cavitation, which allows the erosion by separating grains. At a smaller scale, cavitation bubble implosion tears apart graphite sheets as shown by HRTEM, while HO(*) and H(*) radicals produced from water sonolysis, generate oxidative and reductive reactions on these sheet fragments. Such reactions form smaller species, e.g. dissolved organic matter. The methodology proposed is very sensitive to unambiguously identifying the in situ composition of organic compounds in water. The use of the atmospheric pressure chemical ionization (APCI) Fourier transform mass spectrometry (FTMS) technique minimizes the perturbation of the organic composition and does not require chemical treatment for analysis. The structural features observed in the narrow range (m/z<300) were mainly aromatic compounds (phenol, benzene, toluene, xylene, benzenediazonium, etc.), C(4)-C(6) alkenes and C(2)-C(10) carboxylic acids. Synthesis of small compounds from graphite sonication has never been reported and will probably be helpful to understand the mechanisms involved in high energy radical reactions.