RESUMO
The modeling of the response of living organisms to a change in environment is an important issue of current interest. An example is the effect of ultraviolet radiation on biological systems. In this paper, molecular and analytical identification of Pseudomonas isolate were reported. Then, swarmer Pseudomonas cells were exposed to UVc radiations. The spatiotemporal response of swarmer Pseudomonas, to UVc exposure, was followed. Observing alterations in bacterial membrane integrity by electron microscopy can help to clarify the detailed mechanisms of resistance to UVc. The most evident changes were related to membrane structures. In the cytoplasm, the main finding was the appearance of round mesosomes as intracellular bilayered membranes. Another impact of UVc on Pseudomonas was evident from the appearance of additional membrane structures. In accordance with the viability results, UVc-induced ultrastructural changes of Pseudomonas membrane structures were identified, resulting in cell death, through a multistage model of UVc inactivation.
