[Age composition of the erythrocyte population, ATP and 2,3-diphosphoglycerate composition in erythrocytes in different forms of hemolytic anemia]. / Vozrastnoi sostav éritrotsitarnoi populiatsii, soderzhanie ATF i 2,3-difosfoglitserata v éritrotsitakh pri nekotorykh formakh gemoliticheskikh anemii.

The total erythrocyte population was subdivided into three age groups using as criteria the stability of red blood cells to osmotic shock and the values of glucose-6-phosphate dehydrogenase (G6PD) activity. The "old" erythrocytes constituted about 8%, "mature"--87% and "young"--5% under normal conditions. In hereditary microspherocytosis 70% of erythrocytes possessed a decreased osmotic resistance and higher G6PD activity as compared with normal state. Two categories of erythrocytes was found among "young" cells in Marchiafava-Micheli disease; one of them possessed normal osmotic resistance, the other--decreased osmotic resistance. The age composition of the erythrocyte population was normal in functional hyperbilirubinemia. Content of ATP was decreased in erythrocytes of patients with hereditary microspherocytosis, it was increased in Marchiafava-Micheli disease and approached the normal level in patients with functional hyperbilirubinemia. Concentration of 2,3-diphosphoglycerate was similar to the normal level in erythrocytes of patients with functional hyperbilirubinemia; an inverse correlation was observed between contents of hemoglobin and of 2,3-diphosphoglycerate in blood of patients with hereditary microspherocytosis and with Marchiafava-Michel disease.

[Acetylcholinesterase of erythrocytes in various blood diseases]. / Atsetilkholinesteraza eritrotsitov pri nekotorykh zabolevaniiakh sistemy krovi

Appearance of low molecular components of acetylcholinesterase from erythrocyte membranes was found by gel filtration on Sephadex G-200 and polyacrylamide gel disc electrophoresis. Content of these components was distinctly increased within ghe acute period of Marchiafava-Micheli disease.

[ Change in the protein spectrum of the erythrocytic membranes in certain anemias]. / Izmenenie belkovogo spektra membran éritrotsitov pri nekotorykh anemiiakh

Proteins of human erythrocyte membranes were studied in normal state and in different anaemias. After solubilization of cell "shadows" in phenol-urea-acetic acid-water system in membranes there were found, by means of polyacrylamide gel disc electrophoresis, 20 protein fractions with molecular weight from 48000 to 225000; seven of them glycoproteins. In 9 cases out of 36 a complete or partial disappearance of a glycoprotein component with molecular weight of 170000 was observed in erythrocyte membranes from patients with inherited spherocytosis and Markiafava--Mikely disease.