[ELISA-based determination of natural antibodies to endothelial NO-synthase in human blood and tear]. / Opredelenieestestvennykh antitel k éndotelial'noi NO-sintaze b krovi i sleze cheloveka metodom immunofermentnogo analiza.

Research of natural antibodies (NAA) is a new trend in immunohistochemistry. NAA shape up, in biological fluids, as normal human endogenous low- and high-weight substances, e.g. angiotensin 2, bradykinin and others, and play the regulatory role in homeostasis. Endothelial NO-synthase (e-NOS) is a key enzyme in the path of NO shaping. An e-NOS alteration is important in the pathogenesis of diabetic microangiopathy including retinopathy. We determined, within our case study, the level of NAA to e-NOS in blood and tears of patients with diabetic retinopathy and in donors. The level of NAA to e-NOS was found to be twice higher in tears versus blood in all groups of patients. It depended on specific features and complications of diabetes mellitus.

Content of autoantibodies to bradykinin and beta-amyloid(1-42) as a criterion for biochemical differences between Alzheimer's dementias.

We measured serum content of autoantibodies to beta-amyloid protein Abeta(1-42), its neurotoxic fragment Abeta(25-35), vasopressin, bradykinin, thrombin, antithrombin III, alpha(2)-macroglobulin, and angiotensin II in patients with various forms of Alzheimer's dementias, including presenile and senile dementias of the Alzheimer type. The ratio of antibradykinin and anti-Abeta(1-42) autoantibody contents differed by 39% in these patients. Our results can be used for the development of a new biochemical method for differential diagnostics of dementias of the Alzheimer type.

Antibodies to delta sleep-inducing peptide in ultralow doses: study of the effect by enzyme immunoassay.

The effects of potentiated homeopathic preparations containing antibodies to delta sleep-inducing peptide in ultralow doses were studied by enzyme immunoassay. Experiments were performed with the following immunochemical reagents: antigens of delta sleep-inducing peptide conjugated to various macromolecular carriers and specific antigens. Antibodies to delta sleep-inducing peptide were synthesized in dilutions of C3, C6, C12, C50, and C200. Enzyme immunoassay showed that test preparations of antibodies in dilutions of 1:400-1:3200 produce the combined effect on immune complex formation. The proposed method holds much promise for identification of medicinal preparations in ultralow doses.

[Immunoenzyme analysis of natural antibodies to endogenous bioregulators in patients with systemic lupus erythematosus]. / Immunofermentnyi analiz estestvennykh antitel k éndogennym bioreguliatoram u bol'nykh sistemnoi krasnoi volchankoi.

Serum levels of autoantibodies to endogenous bioregulators (prostaglandin F2 alpha, angiotensin II, epinephrine, bovine serum albumin, dinitrophenol) were measured in patients with systemic and integumental lupus erythematosus and donors and the diagnostic significance of deviations of these levels from the norm was evaluated. A total of 75 patients with lupus erythematosus aged 19-54 years with disease lasting for 0.5 to 18 months were examined. Significant differences between patients and donors were observed as regards virtually all parameters except IgG to angiotensin II.

[Change of natural antibody levels in patients with cardiovascular diseases by the use of anti-atherogenic diets with processed soy product foods]. / Izmenenie urovnia estestvennykh antitel u bol'nykh s serdechno-sosudistymi zabolevaniiami pod vliianiem antiaterogennykh diet s vkliucheniem produktov pererabotki soi.

It was investigated the influence of a diet supplemented with soy oil and soy protein on dynamic of clinic manifestation and immune status patients with IND and HBP. The results of investigations indicated that a (?).

[Chemiluminescent immunoenzyme method for determining gidazepam]. / Khemiliuminestsentnyi immunofermentnyi metod opredeleniia gidazepama.

Competitive solid-phase enzyme immunoassay of gidasepam (a benzodiazepine) in the urine with chemiluminescent detection has been developed. The sensitivity of analysis for gidasepam is 10 ng/ml. Comparative measurements of benzodiazepines in the urine of patients abusing these agents by polarization fluorescence and the proposed method showed good coincidence of the results. The results demonstrate the possibility of using this method in medicine and narcology in combination with other instrumental methods.

[Autoantibodies to vasoactive peptides and angiotensin converting enzyme in patients with systemic diseases of the connective tissue]. / Autoantitela k vazoaktivnym peptidam in angiotenzinprevrashchaiushchemu fermentu u bol'nykh s sistemnymi zabolevaniiami soedinitel'noi tkani.

AIM: To estimate the level of natural autoantibodies (NAAb) to angiotensin-converting enzyme (ACE) and endogenic mediators affecting vascular tone (bradykinin--BK, angiotensin II--AII, vasopressin--VP) as well as the activity of serum ACE in patients with systemic diseases of the connective tissue. MATERIAL AND METHODS: Levels of NAAb were measured by enzyme immunoassay in sera from 30 patients with SLE, 19 patients with rheumatoid arthritis (RA) and 36 patients with scleroderma systematica (SS). Serum from donors served control. IgM NAAb to ACE were measured by a new technique. Serum ACE activity was determined by the initial velocity of hydrolysis reaction using spectrofluometry. RESULTS: IgM NAAb were detected in the sera of both patients and donors. SS patients had the level of NAAb to ACE in diffuse form significantly higher than in limited (p < 0.05). In SLE and SS patients ACE activity was significantly lower (p < 0.05) than in healthy subjects and RA patients. Levels of NAAb to BK was significantly elevated (p < 0.01) in patients with SLE and RA vs donors while to AII in SS patients it was lowered (p < 0.001). Patients with diffuse SS had NAAb to BK higher than patients with SS limited form (p < 0.01). In SLE the lowest levels of NAAb to all the mediators studied were observed in patients with nephritis, for NAAb to VP the differences were significant (p < 0.05). In patients with urinary syndrome concentration of NAAb to BK was significantly higher (p < 0.01), differences between their levels in patients with nephritis and urinary syndrome were also significant (p < 0.05). CONCLUSION: Further studies are needed for specification of physiological or pathological role of NAAb to endogenic mediators.

Autoantibodies to beta-amyloid and neurotransmitters in patients with Alzheimer's disease and senile dementia of the Alzheimer type.

The content of autoantibodies to beta-amyloid protein Abeta(1-42), its neurotoxic fragment Abeta(25-35), and neurotransmitters were studied in the blood of patients with presenile Alzheimer's disease and senile dementia of the Alzheimer type. Significant differences in the relative content of autoantibodies to Abeta(1-42)and autoantibodies to biogenic amines were demonstrated. These results can be used for the development of a biochemical method for differential diagnosis of Alzheimer dementias.

[Immunoglobulins, binding opioid peptides, biogenic amines, and opiates, in substance abusers]. / Immunoglobuliny, sviazyvaiushchie opiodnye peptidy, biogennye aminy i opiaty, u bol'nykh narkomanie.

Immunoglobulins binding morphine, biogenic amines, and an opioid peptide dermorphine were measured by solid-phase enzyme immunoassay in patients abusing narcotics. The patients' ages varied from 20 to 40, with the duration of narcotic use 1-17 years. Narcotic dependence was found to involve increased production of immunoglobulins binding opioid and monoamine neuromediators. Enzyme immunoassay of antibodies to opiates detects latent forms of narcomania in cases when the narcotic is not present in the body. These results serve the basis for creating a new method for analysis of narcotic abuse and evaluation of the duration of their use, which can be used in practical medicine and in forensic medical expert evaluations.

[Immunoenzyme technique for detection of natural antibodies to bradykinin in cardiovascular diseases]. / Immunofermentnyi metod vyiavleniia estestvennykh antitel protiv bradikinina pri serdechno-sosudistykh zabolevaniiakh.

A method for detecting natural antibodies to bradykinin by ELISA has been developed. A polymeric matrix model of peptide antigen has been synthesized for this purpose. Optimal conditions for detecting antigen and antibodies in the sera of donors and cardiovascular patients have been chosen. The level of antibodies to bradykinin is constant in donors and shifted in patients.

[Detection of natural anti-angiotensin converting enzyme antibodies in human serum using immunoenzyme technique ]. / Opredelenie estestvennykh antitel k angiotenzinprevrashchaiushchemu frmentu v syvorotke krovi cheloveka immunofermentnym metodom.

Solid-phase enzyme immunoassay (EIA) was developed for detecting natural antibodies to angiotensine-converting enzyme (ACE). Optimal conditions for detecting natural anti-ACE by EIA in the sera of donors and patients with disorders of arterial pressure are selected. The findings indicate that the level of natural anti-ACE is normally constant, while in the patients it is increased in 50% cases.

[Development of a rapid method, based on the latex agglutination reaction, for diagnosing carnivore plaque]. / Razrabotka methoda ékspress-diagnositki chumy plotoiadnykh na osnove reaktsii lateksnoi aggliutinatsii.

A highly effective rapid method for detecting carnivore plague virus antigen in dogs, based on the latex agglutination test, is developed. Latex conjugate is synthesized on the base of polystyrene suspension, representing a copolymer of styrene and methacrylic acid. The immunospecific reagent is gamma-globulin fraction of rabbit serum containing antibodies specific to carnivore plague virus. The resultant latex conjugate can be used in practical veterinary as a diagnostic agent for screening dogs for carnivore plague virus.

[Detection of rheumatoid factor in the blood of patients by latex agglutination]. / Opredelenie revmatoidnogo faktora v syvorotke krovi bol'nykh metodom lateksnoi aggliutinatsii.

A highly effective rapid method is developed for detecting the rheumatoid factor in human serum. Polystyrene suspension-based latex conjugate is synthesized, representing a styrene and methacrylic acid copolymer. The immunospecific reagent is human IgG. The resultant latex conjugate can be used in practical medicine for analysis of the rheumatoid factor.

[Comparative measurement of antithrombin III by latex agglutination and radial immunodiffusion in patients with peritonitis]. / Sravnitel'noe opredelenie antitrombina III metodom lateksnoi aggliutinatsii i radial'noi immunodiffuzii u bol'nykh peritonitom.

A highly sensitive and rapid method, based on latex agglutination, has been developed for measuring antithrombin III (AT III) in the blood serum of patients and donors. The sensitivity of analysis is 0.6 microgram/ml, time 2 to 3 min. The method is simple, requires no sophisticated equipment, and may be used under field conditions. The results are assessed visually. Immunochemical reagents have been synthesized for the method: latex conjugates and specific antibodies to AT III. The method was tried in patients with peritonitis. An additional criterion for diagnosing the respiratory distress syndrome of adults in this patient population has been developed.

[The production of immunochemical reagents for the determination of C-reactive protein by latex agglutination]. / Poluchenie immunokhimicheskikh reagentov dlia opredeleniia C-reaktivnogo belka metodom lateksnoi aggliutinatsii.

A new simple and convenient method for the detection of C-reactive protein (CRP) in patients' blood sera is based on latex agglutination making use of new compounds obtained by conjugation of IgG fraction containing goat antibodies to CRP with various latex particles. The sensitivity of the methods is 3 mg/ml, this permitting its wide clinical application.

[Polymer-metal complexes of the Mycobacterium protein antigen prevent the dissemination of postvaccinal infection in the T-deficiency state]. / Polimermetallicheskie kompleksy belkovogo antigena mikobakterii predotvrashchaiut disseminatsiiu postvaktsinal'noi infektsii pri T-defitsitnom sostoianii.

Primary obtained triple highly immunogenetic complex consists of nonimmunogenic protein antigen, isolated from the BCG, nontoxic polyelectrolyte and bivalent cuprum ions. Immunization with this complex provides high immunoprotection in the experimented B mice (T-deficit mice) from lethal dissemination BCG. Inoculation of single components of the complex has no protection. Result of these experiments suggest that antigen-polyelectrolyte complexes obtained on the basis of nonimmunogenic protein fraction of the tuberculosis bacilli show the properties of a thymus-independent immunization preparations, and provide immune defense in the T-cell deficit of the immune system.

[Relation of the immunogenicity of artificial antigens to the number of polyelectrolyte-bound protein molecules]. / Zavisimost' immunogennosti iskusstvennykh antigenov ot kolichestva sviazannykh poliélektrolitom belkovykh molekul.

Complexes and covalent conjugates of protein antigens with polyelectrolytes of different molecular mass have been synthesised. The structure and composition of the resulting water-soluble complex particles were determined. Artificial antigen immunogenicity was shown to depend on the amount of protein molecules complexed with polyelectrolytes. Direct correlation between immunostimulating activity of the polymer-carrier, immunogenicity of complex antigens and size-dependent capacity of the polymer molecule to aggregate protein globules has been established.