[The Medical Activity and Satisfaction of Population Need in Medical Care].

The article considers phenomenon of medical activity as a determinant of population demand for medical services and needs in medical care. The medical activity is determined as both a criterion of formation of health of patients by force of active using medical care and a criterion of efficiency of functioning of physicians in satisfaction of needs of patients in medical care. The results are presented related to phenomenon of medical activity using methodology of analysis of opinions of patients and level of medical care appealability of population. Medical activity of population and level of satisfaction of demand and needs in medical services is to be considered as an evidence of commitment of population to treatment and prevention. The investigation of these issues acquires particular specificity in conditions of health care system of large municipal agglomeration with developed network of medical organizations of budget insurance, departmental and private forms of property. In these conditions, patients and population are provided with various possibilities of choosing different forms and types of satisfaction of need in medical care independently of cause and motive of using it that is an advantage for implementing medical activity.

[Antibodies to synthetic fragment 95-123 of the prion protein protect neurons and astrocytes from beta-amyloid toxicity].

Toxic effect of beta-amyloid on brain cells during Alzheimer's disease pathology is known to be associated with oxidative stress, intracellular Ca2+ increase in neurons and astrocytes and activation of reactive oxygen species production. Prion protein is involved in beta-amyloid toxicity. Here we investigated an effect of affinity purified antibodies to synthetic fragment 95-123 of the prion protein on beta-amyloid induced cell death, Ca(2+)-signal, reactive oxygen species production and caspase 3 activation. We have shown that antibodies to fragment 95-123 are able to protect neurons and astrocytes from beta-amyloid induced cell death with no effect on the intracellular Ca2+ concentration altered by beta-amyloid treatment. However, the antibodies significantly reduced Abeta induced reactive oxygen species production in astrocytes and decreased caspase 3 activation in neurons and astrocytes. Thus, induction of antibodies to synthetic fragment 95-123 of the prion protein provides a new approach to anti-AD vaccine design.

[Possible mechanisms of antioxidant activity of glycyrrhizic acid].

Possible mechanisms of antioxidant activity of glycyrrhizinic acid (GA) were studied. GA did not exhibit antiradical properties at the range of concentrations 1-100 mM as was shown in the experiments with stable radical 1,1-Diphenyl-2-picrylhydrazyl. These data were conformed by the study of GA influence on chemiluminescence of luminol in cell-free system with hydrogen peroxide. However, GA decreased generation of reactive oxygen species by PMA-FMLF-activated neutrophils. Addition of GA did not ifluence free radical level in neurons, however, cell preincubation with GA resulted in the decrease of free radicals production and the increase of intracellular glutathione level.

[Regulation of wound process by the method of vacuum therapy in middle-aged and aged patients]. / Reguliatsiia ranevogo protsessa u bol'nykh pozhilogo i starcheskogo vozrasta metodom vakuum-terapii.

Comparative analysis of the wound process in patients of various age groups allowed the conclusion that in elderly and old-age patients the wounds heal longer because of prolongation of the inflammatory phase, poorer local defence reactions, and sluggish reparative processes. This allows scientific substantiation of the postponed removal of the sutures in elderly and old patients. The method of vacuum therapy applied in patients over 60 produces a positive effect in the form of rapid abatement of the inflammatory reaction in the wound. This creates real preconditions for mobilization of the reparative property of the tissues which is diminishes at old age, active proliferation of the connective tissue elements, which results in the formation of a strong and mature scar in a shorter time. Vacuum therapy brings the terms of wound healing in elderly and old patients close to the terms of wound healing by first intention in the young and middle-aged.

[Wound healing after vacuum drainage]. / Zazhivlenie ran v usloviiakh vakuumnogo drenirovaniia.

Vacuum therapy was applied in the management of 63 patients with postoperative ventral hernias, 101 patients with a similar pathological conditions were treated by the traditional method for wound healing. From analysis of the results of treatment and with due consideration for the results of additional methods of examination it was established that vacuum prevents accumulation of exudate and blood clots in the wound cavity and removes the risk medium for the development of pyo-inflammatory complications. Moreover, by promoting the coming together of the wound edges and liquidation of the wound cavity, vacuum therapy affects the metabolic reorganizations in the tissues and leads to stabilization of the regeneration mechanisms. The use of vacuum therapy in the postoperative period creates the possibility of regulating the reparative reactions through an active effect on the healing of the wound in the phase of inflammation, protecting in this manner the biology of the wound process and preventing infectious complications.