[The study of the effect of mono- and polymalt extracts on the psycho-physiological functions of outbred rats in an experiment with course consumption].

The search for natural ingredients that have a certain nutritional value and contribute to the preservation of human health is a very urgent task. The goal of this research was to study the effect of three viscous extracts - two polymalt and one malt extract, on the psychophysiological functions of animals in an experiment with prolonged consumption. Material and methods. The study was conducted on white mongrel male rats weighing 110-130 g, divided into 4 groups of 13 individuals. The 1st group (control) received standard granulated feed without extracts, the 2nd group - the feed with a polymalt grain extract from barley, hulless oats and wheat, the 3rd group - the feed with a polymalt grain extract from barley, hulless oats and rye, the 4th group - the feed with a malt extract from barley grain, in a dose of 2.1 g/kg of body weight. The consumption of the studied extracts in all groups of animals lasted 21 days. The animals were weighed before and after the consumption period and tested using the "Open field" test (research and motor activity were recorded), the «Rotating Rod¼ test (study of locomotor reactions), «Forced Swimming¼ (study of induced depression). Results. The addition of malt and polymalt extracts to the diet of the experimental animals had a beneficial effect on them: the duration of fading as an indicator of depression during swimming test in all experimental rats decreased by 31.4-37.8% with varying degrees of statistical significance. At the same time, a monomalt extract from barley grain and a polymalt extract from grain of barley, naked oats, and rye had a more positive effect on the condition of the animals in the experiment: in the "Rotating Rod" test, the retention time of animals as an indicator of endurance increased by 26.0-27.1%; in the «Open Field¼ test, motor and exploratory activity increased and the time of passive behavior decreased by 35.0-37.4%. Conclusion. The development, production and research of malt extracts is a promising direction that requires the selection of initial grain components taking into account their chemical composition. It is possible to create general restorative supplements on the basis of extracts, as well as to introduce them into alcoholic beverages in order to reduce the toxicity of ethyl alcohol and preserve a number of physiological functions in the human body during alcohol consumption.

[Ways of nutritional value increasing of distillary grain fiber].

In the process of grain processing for ethyl alcohol, practically only carbohydrates are consumed, which are presented mainly in the form of starch. The remaining components (protein, fats, fiber, minerals) in transit pass into the grain fiber remaining after distillation of the alcohol from the mash. Distillery grain fiber surpasses wheat bran in its indicators, since during the processing it is enriched with biomass of alcohol yeast. In addition, there is a technological possibility of its additional enrichment with protein, amino acids, and vitamins due to changes in the modes of alcoholic fermentation. The aim of the work was to assess the influence of the mode and conditions of alcoholic fermentation on the composition of distillery grain fiber. Material and methods. Under laboratory conditions, samples of grain fiber of alcohol production were obtained by the method of fermentation samples from wheat under various fermentation conditions. In the obtained samples, the following parameters were determined: protein according to Barnstein, crude protein, vitamins B1, B2, B6, E, as well as the amino acid composition. The volume fraction of ethyl alcohol, the mass concentration of fermentable carbohydrates, and the concentration of yeast cells were determined in intermediate products of alcohol production. Results and discussion. Studies on the effect of yeast from various manufacturers on fermentation rates, biomass growth and grain fiber composition showed the advantage of race Y-717 in terms of alcohol accumulation (11.5% vol.), Fermentation rate (56 hours) and yeast cell concentration (260 million/cm3), which was 15-30% more than in other options. However, from the point of view of increasing the content of protein according to Barnstein and crude protein in samples of grain fiber with yeast Y-717, an increase of only 3-4% was noted compared with other options. This was associated with a decrease in the concentration of yeast by 72 h of fermentation due to autolysis. Studies on the influence of the initial yeast concentration on the growth of biomass, the fermentation rate and grain fiber indices showed that with an increase in the initial yeast concentration from 15 to 45 million/cm3, the fermentation time reduced to 48 h, the biomass growth at the end of fermentation was 20%, the protein in grain fiber increased by 15%, the content of vitamins B1, B2, B6 and E as well as amino acids increased by 13-17%. Conclusion. According to the results of the studies, the technological possibility of enriching distillery grain fiber with protein, amino acids, and vitamins due to a change in the fermentation process during the processing of grain raw materials to alcohol is shown. In particular, this could be achieved through the use of yeast with a high growth rate, by increasing the concentration of yeast biomass, shortening the fermentation period and preventing yeast autolysis at the maturation stage.

[Promising races of baker's yeast for the production of food ingredients enriched with selenium and chromium].

It is known, that Saccharomycetes can accumulate mineral substances with targeted enrichment of the growth medium. However, the influence of the genetic affiliation of the culture and the technological factors of yeast strains, the composition of growth media on the efficiency of essential trace elements incorporation into the biomass and on the change of theirs intracellular components content have hardly been investigated. In this regard, the aims of this work was to select promising races of yeast Saccharomyces cerevisiae, develop a biotechnological method for obtaining food ingredients enriched with selenium and chromium on their basis, and study their trace element composition. Material and methods. Industrial strains of baker's yeast (Saccharomyces cerevisiae) were used: RCAM 01137, Y-3439 and Y-581. Yeast were grown on malt wort (pH 4.6) with a dry matter content of 12% with the addition of mineral salts in stationary conditions at a temperature of 30 °C for 18 h, after which the yeast biomass was separated by centrifugation. A method for enriching yeast with trace elements has been selected, which consists in the process of culturing cells on malt growth media containing chromium chloride or selenium dioxide in various concentrations. The total protein content was determined by the Kjeldahl method, polysaccharides and ergosterol - by spectrofluorometric method, selenium - by fluorimetric method. The content of trace elements in yeast biomass enriched with chromium was studied by mass spectrometric method with inductively coupled plasma. Results. It was shown that the highest specific growth rate was demonstrated by the yeast strains RCAM 01137 and Y-3439, and the highest level of maltase activity was in the Y-581 strain. It was found that the amount of biomass after cultivation of the yeast S. cerevisiae RCAM 01137 and Y-3439 was 6.00 и 5.42 g/100 cm3, respectively. It was noted, that the yeast S. cerevisiae Y-581 had capability of high synthesis of ergosterol (1.08±0.04%), the level of which was 2 fold higher than other strains. S. cerevisiae RCAM 01137 yeast showed the greatest ability to selenium enrichment, its content in biomass increased 137 fold and amounted to 2740 µg% when cultivated on a medium containing 800 µg/dm3. S. cerevisiae Y-581 yeast strain showed the highest capability to chromium sorption. The chromium concentration in its biomass was 8340 µg% in case of cultivating on a medium containing 750 µg/dm3. The usage of about 2.7 g of selenium enriched yeast biomass, or 1.0 g chromium enriched one, satisfies the daily requirement for these trace elements. Conclusion. Cultivation of S. cerevisiae cells on growth media containing trace elements makes it possible to obtain yeast biomass samples that can be used to obtain food ingredients for creating food products that contribute to the maintaining human health and improve the quality and duration of life.