RESUMO
In the present study, several conventional methods to detect methicillin-resistant Staphylococcus aureus (MRSA) were compared with polymerase chain reaction (PCR) detection of mecA gene-positive isolates. Cefoxitin E-test was also evaluated as a possible phenotypic method of MRSA detection. Oxacillin agar screen and PBP2' latex agglutination methods were found to be more sensitive than oxacillin and cefoxitin disk-diffusion methods. Cefoxitin disk diffusion was found to be the most specific. A combination of oxacillin agar screening with cefoxitin disk diffusion, or oxacillin disk diffusion with PBP2', improved sensitivity and specificity. Cefoxitin E-test with the current break points had low sensitivity and specificity (33.3% and 75%, respectively) for the detection of MRSA. However, changing the break points to Assuntos
Antibacterianos/farmacologia
, Proteínas de Bactérias/genética
, Resistência a Meticilina
, Meticilina/farmacologia
, Reação em Cadeia da Polimerase/métodos
, Staphylococcus aureus/efeitos dos fármacos
, Cefoxitina
, DNA Bacteriano/análise
, DNA Bacteriano/isolamento & purificação
, Humanos
, Testes de Fixação do Látex
, Testes de Sensibilidade Microbiana/métodos
, Proteínas de Ligação às Penicilinas
, Fenótipo
, Infecções Estafilocócicas/microbiologia
, Staphylococcus aureus/classificação
, Staphylococcus aureus/genética
, Staphylococcus aureus/isolamento & purificação