Hematological studies on naturally occurring substances. VI. Effects of an animal crude drug "chan su" (bufonis venenum) on blood coagulation, platelet aggregation, fibrinolysis system and cytotoxicity.

During the screening test of the animal crude drug "Chan su" (Chinese name, toad-cake), the venom of Bufo bufo gargorizans CANTOR (Bufonidae), on blood coagulation, platelet aggregation, fibrinolysis system and cytotoxicity, the ethyl acetate extract showed promotive action on platelet aggregation and remarkable cytotoxic activity on HeLa-S3 cells. Nine kinds of bufadienolides were isolated from the ethyl acetate extract by bioactivity-guided fractionation and were identified by chemical and spectral analysis.

[Absorption, excretion, distribution and metabolism of 3H-labelled pepleomycin sulfate (author's transl)].

Absorption, excretion, distribution and metabolism of pepleomycin sulfate (NK631) were investigated in rats after intravenous administration of 3H-NK631 by whole-body autoradiography, CM-Sephadex column chromatography and high performance liquid chromatography. The highest radioactivity was found in kidney, followed by cartilage, blood, lung, esophagus, adrenal gland and skin at 0.5 hour after the administration and little radioactivity was observed in central nerve system. The NK631 and deamide NK631 were identified as major (90%) and minor metabolites in the 24-hour urine.

Biosynthesis of agr-Ecdysone by Prothoracic Glands in vitro.

An in vitro study in which isolated prothoracic glands of the Bombyx silkworm were cultured has provided definite evidence that the prothoracic gland is the site where molting hormone is synthesized. The hormone behaved very similarly to free ecdysone on thin-layer chromatography. Analysis by liquid chromatography and mass fragmentography revealed that the hormone is identical with alpha-ecdysone.