Activation of cross-reactive mucosal T and B cell responses in human nasopharynx-associated lymphoid tissue in vitro by Modified Vaccinia Ankara-vectored influenza vaccines.

UNLABELLED: Recent efforts have been focused on the development of vaccines that could induce broad immunity against influenza virus, either through T cell responses to conserved internal antigens or B cell response to cross-reactive haemagglutinin (HA). We studied the capacity of Modified Vaccinia Ankara (MVA)-vectored influenza vaccines to induce cross-reactive immunity to influenza virus in human nasopharynx-associated lymphoid tissue (NALT) in vitro. Adenotonsillar cells were isolated and stimulated with MVA vaccines expressing either conserved nucleoprotein (NP) and matrix protein 1 (M1) (MVA-NP-M1) or pandemic H1N1 HA (MVA-pdmH1HA). The MVA vaccine uptake and expression, and T and B cell responses were analyzed. MVA-vectored vaccines were highly efficient infecting NALT and vaccine antigens were highly expressed by B cells. MVA-NP-M1 elicited T cell response with greater numbers of IFNγ-producing CD4+ T cells and tissue-resident memory T cells than controls. MVA-pdmH1HA induced cross-reactive anti-HA antibodies to a number of influenza subtypes, in an age-dependent manner. The cross-reactive antibodies include anti-avian H5N1 and mainly target HA2 domain. CONCLUSION: MVA vaccines are efficient in infecting NALT and the vaccine antigen is highly expressed by B cells. MVA vaccines expressing conserved influenza antigens induce cross-reactive T and B cell responses in human NALT in vitro, suggesting the potential as mucosal vaccines for broader immunity against influenza.

Can apneagraphy change our approach in management of snoring and sleep apnoea?

UNLABELLED: To evaluate role of overnight domiciliary apneagraph in diagnosing severity of sleep apnoea. Prospective audit of 37 patients with Epworth sleepiness score over ten and all patients with history of sleep apnoea presenting to ENT outpatient clinic investigated with apneagraph. Data analysed initially with pulse oximetry findings alone followed by analysis with full apneagraph findings. RESULTS: Data analysed with pulse oximetry alone showed 31 patients to be simple snorers, but on analysis with apneagraph, 11 of these were shown to be suffering from moderate and one with severe sleep apnoea. There was no correlation between Epworth sleepiness score and severity of sleep apnoea. CONCLUSION: Management plan can be more evidence based by using apneagraph as a mini sleep study in investigating patients with history of obstructive sleep apnoea. Apneagraph could also be used in diagnosing the level of obstruction in snorers; however, this aspect needs further studying.

Hearing rehabilitation with middle ear implants: an overview.

Implant otology has been a rapidly growing field with growing needs and development Over a few decades. The introduction of middle ear implants in the last 2 decades has seen good short-term success in rehabilitation of patients with deafness ranging from mild to severe degree. Middle ear implants are alternatives to conventional hearing aids and bone anchored hearing aids; together these aids and implants provide patients with a range of available options in augmentation of hearing. This article describes the types of middle ear implants and their mechanism of action, the surgical and audio-logical criteria for implantation, their limitations and reported outcomes.