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1.
Physiol Behav ; 282: 114582, 2024 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-38750805

RESUMO

Food restriction can have profound effects on various aspects of behavior, physiology, and morphology. Such effects might be amplified in animals that are highly active, given that physical activity can represent a substantial fraction of the total daily energy budget. More specifically, some effects of food restriction could be associated with intrinsic, genetically based differences in the propensity or ability to perform physical activity. To address this possibility, we studied the effects of food restriction in four replicate lines of High Runner (HR) mice that have been selectively bred for high levels of voluntary wheel running. We hypothesized that HR mice would respond differently than mice from four non-selected Control (C) lines. Healthy adult females from generation 65 were housed individually with wheels and provided access to food and water ad libitum for experimental days 1-19 (Phase 1), which allowed mice to attain a plateau in daily running distances. Ad libitum food intake of each mouse was measured on days 20-22 (Phase 2). After this, each mouse experienced a 20 % food restriction for 7 days (days 24-30; Phase 3), and then a 40 % food restriction for 7 additional days (days 31-37; Phase 4). Mice were weighed on experimental days 1, 8, 9, 15, 20, and 23-37 and wheel-running activity was recorded continuously, in 1-minute bins, during the entire experiment. Repeated-measures ANOVA of daily wheel-running distance during Phases 2-4 indicated that HR mice always ran much more than C, with values being 3.29-fold higher during the ad libitum feeding trial, 3.58-fold higher with -20 % food, and 3.06-fold higher with -40 % food. Seven days of food restriction at -20 % did not significantly reduce wheel-running distance of either HR (-5.8 %, P = 0.0773) or C mice (-13.3 %, P = 0.2122). With 40 % restriction, HR mice showed a further decrease in daily wheel-running distance (P = 0.0797 vs. values at 20 % restriction), whereas C mice did not (P = 0.4068 vs. values at 20 % restriction) and recovered to levels similar to those on ad libitum food (P = 0.3634). For HR mice, daily running distances averaged 11.4 % lower at -40 % food versus baseline values (P = 0.0086), whereas for C mice no statistical difference existed (-4.8 %, P = 0.7004). Repeated-measures ANOVA of body mass during Phases 2-4 indicated a highly significant effect of food restriction (P = 0.0001), but no significant effect of linetype (P = 0.1764) and no interaction (P = 0.8524). Both HR and C mice had a significant reduction in body mass only when food rations were reduced by 40 % relative to ad libitum feeding, and even then the reductions averaged only -0.60 g for HR mice (-2.6 %) and -0.49 g (-2.0 %) for C mice. Overall, our results indicate a surprising insensitivity of body mass to food restriction in both high-activity (HR) and ordinary (C) mice, and also insensitivity of wheel running in the C lines of mice, thus calling for studies of compensatory mechanisms that allow this insensitivity.


Assuntos
Peso Corporal , Ingestão de Alimentos , Atividade Motora , Corrida , Animais , Camundongos , Feminino , Peso Corporal/fisiologia , Peso Corporal/genética , Ingestão de Alimentos/fisiologia , Ingestão de Alimentos/genética , Atividade Motora/fisiologia , Corrida/fisiologia , Privação de Alimentos/fisiologia , Seleção Artificial , Análise de Variância
2.
J Food Biochem ; 45(11): e13966, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34658045

RESUMO

Green bell pepper is highly susceptible to low temperature. The activation of the enzymatic antioxidant system plays a determining role in tolerance to chilling injury (CI). Immersion in hot water for short time previous to storage at low temperature induces tolerance to this disorder. However, there is a lack of information about the induction of chilling tolerance in bell pepper by hot water and its relationship with the enzymatic antioxidant system. We evaluated the effect of three immersion times (T, 1-, 2-, 3-min) in hot water (HW, 53°C) on the reduction of CI in bell pepper and its relationship with the enzymatic antioxidant system during storage at 5°C and 21°C. The use of hot water for 1-, 2- or 3-min reduced the decay and CI indexes, maintained quality parameters, ascorbic acid, and total phenolics content. The storage at 5°C by itself activated the enzymatic antioxidant system. The use of HWT 1-, 2-, and 3-min helped to increase this effect, especially by HWT2 . PRACTICAL APPLICATIONS: The application of a treatment with hot water for short times in fruit sensitive to chilling injury is undoubtedly a viable alternative to increase their tolerance and commercialization. In this study, the application of a hot water treatment for 1-, 2- or 3-min in bell pepper reduced the deterioration and susceptibility to chilling injury and stimulated the enzymatic antioxidant system. In this sense, agricultural producers can take advantage of this treatment to prolong the storage period of the fruit maintaining its quality and improving its commercialization.


Assuntos
Capsicum , Antioxidantes , Ácido Ascórbico , Temperatura Baixa , Frutas
3.
Behav Processes ; 135: 56-65, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-27908664

RESUMO

Do animals evolve (coadapt) to choose diets that positively affect their performance abilities? We addressed this question from a microevolutionary perspective by examining preference for Western diet (WD: high in fat and sugar, but lower in protein) versus standard rodent chow in adults of both sexes from 4 lines of mice selectively bred for high levels of voluntary wheel running (High Runner or HR lines) and 4 non-selected control (C) lines. We also assessed whether food preference or substitution affects physical activity (wheel running and/or spontaneous physical activity [SPA] in the attached home cages). In experiment 1 (generation 56), mice were given 6days of wheel acclimation (as is used routinely to pick breeders in the selection experiment) prior to a 2-day food choice trial. In experiment 2 (generation 56), 17days of wheel acclimation allowed mice to reach a stable level of daily running, followed by a 7-day food-choice trial. In experiment 3 (generation 58), mice had 6days of wheel acclimation with standard chow, after which half were switched to WD for two days. In experiment 1, WD was highly preferred by all mice, with somewhat greater preference in male C mice. In experiment 2, wheel running increased and SPA decreased continuously for the first 14days of adult wheel testing, followed by 3-day plateaus in both. During the subsequent 7-day food choice trial, HR mice of both sexes preferred WD significantly more than did C mice; moreover, wheel running increased in all groups except males from C lines, with the increase being significantly greater in HR than C, while SPA declined further in all groups. In experiment 3, the effect of being switched to WD depended on both linetype and sex. On standard chow, only HR females showed a significant change in wheel running during nights 7+8, increasing by 10%. In contrast, when switched to WD, C females (+28%), HR females (+33%), and HR males (+10%) all significantly increased their daily wheel-running distances. Our results show for the first time that dietary preferences can coadapt in response to selection on activity levels.


Assuntos
Comportamento Animal/fisiologia , Dieta Ocidental , Interação Gene-Ambiente , Condicionamento Físico Animal/fisiologia , Corrida/fisiologia , Animais , Feminino , Genótipo , Masculino , Camundongos , Camundongos Endogâmicos ICR , Fatores Sexuais
4.
Physiol Biochem Zool ; 89(6): 546-552, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27792529

RESUMO

In vertebrates, acute stressors-although short in duration-can influence physiology and behavior over a longer time course, which might have important ramifications under natural conditions. In laboratory rats, for example, acute stress has been shown to increase anxiogenic behaviors for days after a stressor. In this study, we quantified voluntary wheel-running behavior for 22 h following a restraint stress and glucocorticoid levels 24 h postrestraint. We utilized mice from four replicate lines that have been selectively bred for high voluntary wheel-running activity (HR mice) for 60 generations and their nonselected control (C) lines to examine potential interactions between exercise propensity and sensitivity to stress. Following 6 d of wheel access on a 12L∶12D photo cycle (0700-1900 hours, as during the routine selective breeding protocol), 80 mice were physically restrained for 40 min, beginning at 1400 hours, while another 80 were left undisturbed. Relative to unrestrained mice, wheel running increased for both HR and C mice during the first hour postrestraint (P < 0.0001) but did not differ 2 or 3 h postrestraint. Wheel running was also examined at four distinct phases of the photoperiod. Running in the period of 1600-1840 hours was unaffected by restraint stress and did not differ statistically between HR and C mice. During the period of peak wheel running (1920-0140 hours), restrained mice tended to run fewer revolutions (-11%; two-tailed P = 0.0733), while HR mice ran 473% more than C (P = 0.0008), with no restraint × line type interaction. Wheel running declined for all mice in the latter part of the scotophase (0140-0600 hours), restraint had no statistical effect on wheel running, but HR again ran more than C (+467%; P = 0.0122). Finally, during the start of the photophase (0720-1200 hours), restraint increased running by an average of 53% (P = 0.0443) in both line types, but HR and C mice did not differ statistically. Mice from HR lines had statistically higher plasma corticosterone concentrations than C mice, with no statistical effect of restraint and no interaction between line type and restraint. Overall, these results indicate that acute stress can affect locomotor activity (or activity patterns) for many hours, with the most prominent effect being an increase in activity during a period of typical inactivity at the start of the photophase, 15-20 h poststressor.


Assuntos
Atividade Motora/fisiologia , Restrição Física/fisiologia , Estresse Fisiológico/fisiologia , Animais , Masculino , Camundongos , Camundongos Endogâmicos , Atividade Motora/genética , Fatores de Tempo
5.
Physiol Behav ; 149: 279-86, 2015 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-26079567

RESUMO

The purpose of this study was to evaluate the effects of early-life exercise on adult physical activity (wheel running, home-cage activity), body mass, food consumption, and circulating leptin levels in males from four replicate lines of mice selectively bred for high voluntary wheel running (High Runner or HR) and their four non-selected control (C) lines. Half of the mice were given wheel access shortly after weaning for three consecutive weeks. Wheel access was then removed for 52 days, followed by two weeks of adult wheel access for all mice. A blood sample taken prior to adult wheel testing was analyzed for circulating leptin concentration. Early-life wheel access significantly increased adult voluntary exercise on wheels during the first week of the second period of wheel access, for both HR and C mice, and HR ran more than C mice. During this same time period, activity in the home cages was not affected by early-age wheel access, and did not differ statistically between HR and C mice. Throughout the study, all mice with early wheel access had lower body masses than their sedentary counterparts, and HR mice had lower body masses than C mice. With wheel access, HR mice also ate significantly more than C mice. Early-life wheel access increased plasma leptin levels (adjusted statistically for fat-pad mass as a covariate) in C mice, but decreased them in HR mice. At sacrifice, early-life exercise had no statistically significant effects on visceral fat pad, heart (ventricle), liver or spleen masses (all adjusted statistically for variation in body mass). Results support the hypothesis that early-age exercise in mice can have at least transitory positive effects on adult levels of voluntary exercise, in addition to reducing body mass, and may be relevant for the public policy debates concerning the importance of physical education for children.


Assuntos
Atividade Motora/fisiologia , Condicionamento Físico Animal/fisiologia , Animais , Índice de Massa Corporal , Peso Corporal , Ingestão de Alimentos/fisiologia , Leptina/sangue , Masculino , Camundongos , Fenótipo , Fatores de Tempo
6.
Physiol Behav ; 149: 86-94, 2015 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-26025787

RESUMO

We evaluated the effect of voluntary exercise on spontaneous physical activity (SPA) and food consumption in mice from 4 replicate lines bred for 57 generations for high voluntary wheel running (HR) and from 4 non-selected control (C) lines. Beginning at ~24 days of age, mice were housed in standard cages or in cages with attached wheels. Wheel activity and SPA were monitored in 1-min intervals. Data from the 8th week of the experiment were analyzed because mice were sexually mature and had plateaued in body mass, weekly wheel running distance, SPA, and food consumption. Body mass, length, and masses of the retroperitoneal fat pad, liver, and heart were recorded after the 13th week. SPA of both HR and C mice decreased with wheel access, due to reductions in both duration and average intensity of SPA. However, total activity duration (SPA+wheel running; min/day) was ~1/3 greater when mice were housed with wheels, and food consumption was significantly increased. Overall, food consumption in both HR and C mice was more strongly affected by wheel running than by SPA. Duration of wheel running had a stronger effect than average speed, but the opposite was true for SPA. With body mass as a covariate, chronic wheel access significantly reduced fat pad mass and increased heart mass in both HR and C mice. Given that both HR and C mice housed with wheels had increased food consumption, the energetic cost of wheel running was not fully compensated by concomitant reductions in SPA. The experiment demonstrates that both duration and intensity of both wheel running and SPA were significant predictors of food consumption. This sort of detailed analysis of the effects of different aspects of physical activity on food consumption has not previously been reported for a non-human animal, and it sets the stage for longitudinal examination of energy balance and its components in rodent models.


Assuntos
Ingestão de Alimentos/fisiologia , Atividade Motora/fisiologia , Condicionamento Físico Animal/fisiologia , Seleção Genética , Tecido Adiposo/fisiologia , Animais , Animais Recém-Nascidos , Índice de Massa Corporal , Ingestão de Alimentos/genética , Metabolismo Energético/genética , Feminino , Camundongos , Atividade Motora/genética
7.
Mech Dev ; 134: 16-30, 2014 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-25217814

RESUMO

The myosin heavy chain (MyHC) isoform composition of locomotor and non-locomotor muscles of mini-muscle mice were assessed at the protein and mRNA levels in both adult and juvenile (21 day old) mice. Mini-muscle mice are one outcome of a replicated artificial selection experiment in which four lines of mice were bred for high voluntary wheel running (HR lines). Two of the lines responded with an increase in frequency of a single nucleotide polymorphism in an intron in the MyHC-2b gene (myh4) that when homozygous causes a dramatic reduction in triceps surae mass. We found that both locomotor and non-locomotor muscles of adult mini-muscle mice displayed robust reductions, but not elimination, of the MyHC-2b isoform at both the protein and mRNA levels, with commensurate increases in MyHC-2x and sometimes MyHC-2a, as compared with either a line of HR mice that does not display the mini-muscle phenotype or inbred C57Bl6 mice. Immunohistochemical analyses revealed that locomotor muscles of mini-muscle mice contain fibers that express the MyHC-2b isoform, which migrates normally in SDS-PAGE gels. However, these MyHC-2b positive fibers are generally smaller than the surrounding fibers and smaller than the MyHC-2b positive fibers of non-mini-muscle mice, resulting in characteristically fast muscles that lack a substantial MyHC-2b positive (superficial) region. In contrast, the masseter, a non-locomotor muscle of mini-muscle mice contained MyHC-2b positive fibers that stained more lightly for MyHC-2b, but appeared normal in size and distribution. In adults, many of the MyHC-2b positive fibers in the mini-muscle mice also display central nuclei. Only a small proportion of small MyHC-2b fibers in mini-muscle mice stained positive for the neural cell adhesion molecule, suggesting that anatomical innervation was not compromised. In addition, weanling (21 day old), but not 5 day old mice, displayed alterations in MyHC isoform content at both the protein and mRNA levels, including reductions in MyHC-2b and elevations in the neonatal (a.k.a. perinatal) isoform of MyHC. Collectively, these data demonstrate that the alterations in the expression of MyHC-2b are not restricted to locomotor muscles and therefore are not caused simply by any possible alterations in locomotor activity (e.g., reduced general activity in home cages). The differences in MyHC composition do not appear to result from a defect in innervation of the MyHC-2b fibers, but may result from an inefficient neonatal-to-2b MyHC isoform transition during development and are consistent with a selective lack of maturation of MyHC-2b fibers caused by reduced expression of the MyHC-2b (myh4) gene.


Assuntos
Fibras Musculares Esqueléticas/metabolismo , Músculo Esquelético/metabolismo , Cadeias Pesadas de Miosina/metabolismo , Condicionamento Físico Animal/fisiologia , Isoformas de Proteínas/metabolismo , Animais , Camundongos , Atividade Motora/fisiologia
8.
J Exp Biol ; 216(Pt 22): 4212-21, 2013 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-23948476

RESUMO

Four lines of mice bred for high voluntary wheel running (HR lines) have high baseline circulating corticosterone levels and increased daily energy expenditure as compared with four non-selected control (C) lines. High corticosterone may suppress immune function and competing energy demands may limit ability to mount an immune response. We hypothesized that HR mice have a reduced immune response and therefore a decreased ability to fight an infection by Trichinella spiralis, an ecologically relevant nematode common in mammals. Infections have an acute, intestinal phase while the nematode is migrating, reproducing and traveling throughout the bloodstream, followed by a chronic phase with larvae encysted in muscles. Adult males (generation 55 of the selection experiment) were sham-infected or infected by oral gavage with ~300 J1 T. spiralis larvae. During the chronic phase of infection, mice were given wheel access for 6 days, followed by 2 days of maximum aerobic performance trials. Two weeks post-infection, infected HR had significantly lower circulating immunoglobulin E levels compared with infected C mice. However, we found no statistical difference between infected HR and C mice in numbers of encysted larvae within the diaphragm. As expected, both voluntary running and maximum aerobic performance were significantly higher in HR mice and lower in infected mice, with no line type-by-infection interactions. Results complement those of previous studies suggesting decreased locomotor abilities during the chronic phase of T. spiralis infection. However, despite reduced antibody production, breeding for high voluntary wheel exercise does not appear to have a substantial negative impact on general humoral function.


Assuntos
Adaptação Biológica/fisiologia , Atividade Motora/fisiologia , Trichinella spiralis/imunologia , Triquinelose/imunologia , Triquinelose/fisiopatologia , Análise de Variância , Animais , Cruzamento , Corticosterona/sangue , Imunoglobulina E/sangue , Masculino , Camundongos , Camundongos Endogâmicos , Atividade Motora/genética , Consumo de Oxigênio/fisiologia , Condicionamento Físico Animal/fisiologia
9.
J Exp Biol ; 215(Pt 10): 1651-61, 2012 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-22539732

RESUMO

Chronic increases in circulating corticosterone (CORT) generally suppress immune function, but it is not known whether evolved increases necessarily have similar adverse effects. Moreover, the evolution of immune function might be constrained by the sharing of signaling molecules, such as CORT, across numerous physiological systems. Laboratory house mice (Mus domesticus Linnaeus) from four replicate lines selectively bred for high voluntary wheel running (HR lines) generally had baseline circulating CORT approximately twofold higher than in four non-selected control (C) lines. To test whether elevated baseline CORT suppresses the inflammatory response in HR mice, we injected females with lipopolysaccharide (LPS). All mice injected with LPS exhibited classic signs of an inflammatory response, including sickness behavior, loss of body mass, reduced locomotor activity (i.e. voluntary wheel running), enlarged spleens and livers, elevated hematocrit and elevated inflammatory cytokines. However, as compared with C mice, the inflammatory response was not suppressed in HR mice. Our results, and those of a previous study, suggest that selective breeding for high voluntary exercise has not altered immune function. They also suggest that the effects of evolved differences in baseline CORT levels may differ greatly from effects of environmental factors (often viewed as 'stressors') that alter baseline CORT during an individual's lifetime. In particular, evolved increases in circulating levels of 'stress hormones' are not necessarily associated with detrimental suppression of the inflammatory response, presumably as a result of correlated evolution of other physiological systems (counter-measures). Our results have important implications for the interpretation of elevated stress hormones and of immune indicators in natural populations.


Assuntos
Condicionamento Físico Animal , Animais , Evolução Biológica , Peso Corporal , Cruzamento , Corticosterona/sangue , Citocinas/metabolismo , Feminino , Hematócrito , Sistema Imunitário/fisiologia , Inflamação , Análise dos Mínimos Quadrados , Lipopolissacarídeos/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos ICR , Atividade Motora/fisiologia , Fenótipo , Corrida , Fatores de Tempo
10.
J Exp Biol ; 214(Pt 2): 206-29, 2011 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-21177942

RESUMO

Mammals expend energy in many ways, including basic cellular maintenance and repair, digestion, thermoregulation, locomotion, growth and reproduction. These processes can vary tremendously among species and individuals, potentially leading to large variation in daily energy expenditure (DEE). Locomotor energy costs can be substantial for large-bodied species and those with high-activity lifestyles. For humans in industrialized societies, locomotion necessary for daily activities is often relatively low, so it has been presumed that activity energy expenditure and DEE are lower than in our ancestors. Whether this is true and has contributed to a rise in obesity is controversial. In humans, much attention has centered on spontaneous physical activity (SPA) or non-exercise activity thermogenesis (NEAT), the latter sometimes defined so broadly as to include all energy expended due to activity, exclusive of volitional exercise. Given that most people in Western societies engage in little voluntary exercise, increasing NEAT may be an effective way to maintain DEE and combat overweight and obesity. One way to promote NEAT is to decrease the amount of time spent on sedentary behaviours (e.g. watching television). The effects of voluntary exercise on other components of physical activity are highly variable in humans, partly as a function of age, and have rarely been studied in rodents. However, most rodent studies indicate that food consumption increases in the presence of wheels; therefore, other aspects of physical activity are not reduced enough to compensate for the energetic cost of wheel running. Most rodent studies also show negative effects of wheel access on body fat, especially in males. Sedentary behaviours per se have not been studied in rodents in relation to obesity. Several lines of evidence demonstrate the important role of dopamine, in addition to other neural signaling networks (e.g. the endocannabinoid system), in the control of voluntary exercise. A largely separate literature points to a key role for orexins in SPA and NEAT. Brain reward centers are involved in both types of physical activities and eating behaviours, likely leading to complex interactions. Moreover, voluntary exercise and, possibly, eating can be addictive. A growing body of research considers the relationships between personality traits and physical activity, appetite, obesity and other aspects of physical and mental health. Future studies should explore the neurobiology, endocrinology and genetics of physical activity and sedentary behaviour by examining key brain areas, neurotransmitters and hormones involved in motivation, reward and/or the regulation of energy balance.


Assuntos
Ingestão de Alimentos , Metabolismo Energético , Exercício Físico , Atividade Motora , Obesidade/metabolismo , Animais , Ingestão de Energia , Humanos , Neurobiologia , Roedores
11.
Behav Genet ; 41(4): 615-24, 2011 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21184162

RESUMO

When populations with similar histories of directional selection are crossed, their offspring may differ in mean phenotype as compared with the average for the parental populations, often exhibiting enhancement of the mean phenotype (termed heterosis or hybrid vigor). We tested for heterosis in a cross of two replicate lines of mice selectively bred for high voluntary wheel running for 53 generations. Mice were paired to produce four sets of F1 offspring: two purebred High Runner (HR) lines and the hybrid reciprocal crosses. The purebred HR showed statistically significant, sex-dependent differences in body mass, wheel revolutions, running duration, mean running speed, and (controlling for body mass) organ masses (heart ventricles, liver, spleen, triceps surae muscle). Hybrid males ran significantly more revolutions than the purebred males, mainly via increased running speeds, but hybrid females ran intermediate distances, durations, and speeds, as compared with the purebred females. In both sexes, ventricles were relatively smaller in hybrids as compared with purebred HR. Overall, our results demonstrate differential and sex-specific responses to selection in the two HR lines tested, implying divergent genetic architectures underlying high voluntary exercise.


Assuntos
Genética Comportamental , Atividade Motora/genética , Alelos , Animais , Comportamento Animal , Peso Corporal , Cruzamentos Genéticos , Feminino , Masculino , Camundongos , Modelos Genéticos , Tamanho do Órgão , Fenótipo , Fatores Sexuais , Fatores de Tempo
12.
Rev. cient. (Maracaibo) ; 14(1): 59-67, ene.-feb. 2004. tab
Artigo em Espanhol | LILACS | ID: lil-423427

RESUMO

El principal producto lácteo fluido que se consume en Venezuela es la leche pasteurizada, esta es suceptible a la contaminación bacteriana por diversas fuentes, anteriores y posteriores a la pasteurización. Con el fin de evaluar las condiciones microbiológicas de cinco marcas de elevado consumo (A, B, C, D y E), se analizaron 216 muestras obtenidas en panaderías y supermercados distribuidas en la ciudad de Maracaibo, a las cuales se les practicaron las pruebas de recuento de aeróbios mesófilos (RAM), coliformes, termodúricos, psicrótrofos, pruebas para evaluar la eficiencia de la pasteurización, entre otras. Entre los resultados obtenidos se encontró un RAM promedio de 4,3 x 10 elevado a la 4 ufc/mL, resultando el 14,35 por ciento de las muestras superando el límite establecido por la Comisión Venezolana de Normas Industriales (COVENIN). Los coliformes promediaron 2,8 x 10² ufc/mL, con 50,93 por ciento de las muestras por encima de 1,0 x 10² ufc/mL. Los termodúricos y psicrótrofos arrojaron promedios de 5,9 x 10³ ufc/mL y 8,7 x 10² ufc/mL respectivamente. Se obtuvo una elevada carga bacteriana, más del 99 por ciento de las muestras resultaron negativas a la prueba de peroxidasa, indicando un marcado sobrecalentamiento en el procesamiento térmico, por lo cual se concluye que las muestras analizadas fueron con mucha frecuencia, contamindas después de la pasteurización, probablemente por fallas en la aplicación de las buenas prácticas de manufactura


Assuntos
Microbiologia de Alimentos , Qualidade dos Alimentos , Leite , Perfis Sanitários , Tecnologia de Alimentos , Venezuela
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