The Impact of Solid-Phase Fermentation on Flavonoids, Phenolic Acids, Tannins and Antioxidant Activity in Chamerion angustifolium (L.) Holub (Fireweed) Leaves.

At present, the consumption of medical plants and functional foods is growing across the whole world. Fireweed (Chamerion angustifolium (L.) Holub), an important medicinal plant that has various pharmacological effects (antioxidant, anti-inflammatory, anticancer and others), can improve the state of health and well-being and reduce the risk of various diseases. The aim of this work was to investigate polyphenols (flavonoids, phenolic acids and tannins) and antioxidant activity in fireweed leaves fermented for 24, 48 and 72 h in solid-phase fermentation under aerobic and anaerobic conditions. High-performance liquid chromatography (HPLC) for polyphenols and the spectrophotometric method based on quenching of stable colored radical (ABTS•+) for antioxidant activity determinations were used. The results showed that the highest amounts of total polyphenols, total flavonoids and tannin oenothein B in the dried matter were found after 72 h and the highest total phenolic acids after 48 h of anaerobic solid-phase fermentation. The highest antioxidant activity was found after 72 h of solid-phase fermentation under aerobic conditions.

Relationship between CO2 emissions and soil properties of differently tilled soils.

Different tillage technologies have different effects on CO2 emissions from soil. Unfortunately, little information exists about the impact of different types of tillage as compared with no-tillage, and the main controls. The aim of this research is to determine the relationship between physicomechanical, chemical and biological properties of soil and CO2 emissions from differently tilled soils under the climatic conditions of central Lithuania before and after autumn tillage. The studies were conducted in 2009-2012 and 2014 at the Experimental Station of Aleksandras Stulginskis University in Central Lithuania. Different tillage technologies were applied: deep ploughing at 23-25 cm depth (DP); shallow ploughing at 12-15 cm depth (SP); deep cultivation with a cultivator at 25-27 cm depth (DC); shallow cultivation with a disc harrow at 12-15 cm depth (SC); and no-tillage (NT). The correlation of physicomechanical, chemical and biological soil properties with CO2 emissions was determined. During all the experimental period total CO2 emissions from soil in DP, SP, DC, SC and NT technologies were respectively 6.05, 4.25, 4.97, 4.42, 3.94 µmol m-2 s-1 before autumn soil tillage and 29.88, 22.50, 16.73, 13.72, 10.00 µmol m-2 s-1 after autumn tillage. Negative correlation between soil temperature and CO2 emissions before the autumn tillage from soil was evidenced (r = -0.98). A strong negative correlation between soil respiration and total soil porosity was observed. Correlation between aeration soil porosity and CO2 emissions was strong. After autumn tillage, the strongest correlations were found between soil penetration resistance and respiration in the upper (r = -0.75) and deeper (r = -0.71) layers. In autumn, a significant strong correlation (r = 0.78) between soil respiration and aeration porosity was obtained in the upper soil layer under ploughing or cultivation. This study revealed that CO2 emissions were significantly higher immediately after autumn ploughing technologies compared to deep and shallow cultivation and no-tillage.

The influence of biopreparations on the reduction of energy consumption and CO2 emissions in shallow and deep soil tillage.

The application of innovation in agriculture technologies is very important for increasing the efficiency of agricultural production, ensuring the high productivity of plants, production quality, farm profitability, the positive balance of used energy, and the requirements of environmental protection. Therefore, it is a scientific problem that solid and soil surfaces covered with plant residue have a negative impact on the work, traction resistance, energy consumption, and environmental pollution of tillage machines. The objective of this work was to determine the dependence of the reduction of energy consumption and CO2 gas emissions on different biopreparations. Experimental research was carried out in a control (SC1) and seven different biopreparations using scenarios (SC2-SC8) using bacterial and non-bacterial biopreparations in different consistencies (with essential and mineral oils, extracts of various grasses and sea algae, phosphorus, potassium, humic and gibberellic acids, copper, zinc, manganese, iron, and calcium), estimating discing and plowing as the energy consumption parameters of shallow and deep soil tillage machines, respectively. CO2 emissions were determined by evaluating soil characteristics (such as hardness, total porosity and density). Meteorological conditions such average daily temperatures (2015-20.3 °C; 2016-16.90 °C) and precipitations (2015-6.9 mm; 2016-114.9 mm) during the month strongly influenced different results in 2015 and 2016. Substantial differences between the averages of energy consumption identified in approximately 62% of biological preparation combinations created usage scenarios. Experimental research established that crop field treatments with biological preparations at the beginning of vegetation could reduce the energy consumption of shallow tillage machines by up to approximately 23%, whereas the energy consumption of deep tillage could be reduced by up to approximately 19.2% compared with the control treatment. The experimental research results reveal the reduction of CO2 emissions in shallow tillage to approximately 20.14% (and that in deep tillage to approximately 19.16%) when works were performed by different biological preparation usage scenarios. This experimental research demonstrates the efficient use of the special adaptation of a new biotechnological method for the reduction of the energy consumption and CO2 gas emissions of agricultural machinery.