Students' passion for their work filled me with hope for future.

On January 9, I joined the thousands of protestors marching through central London to protect the NHS bursary, and on the night of January 11, I sat in Westminster Hall listening to MPs debate the e-petition 'Keep the NHS Bursary'.

The setting of nurse staffing levels must be evidence-based.

The suspension of NICE's vital work on safe nurse staffing levels is a damaging step back, not only for nursing but for all who use the NHS and care about its future.

A video ethnography approach for linking naturalistic behaviors to research constructs of neurocognition in schizophrenia.

Few methods are available to explore the impact of neurocognition in schizophrenia on behaviors performed in usual contexts. The authors developed a video ethnography approach to examine the relationship between naturalistic behaviors and research constructs of neurocognition. Video ethnographers accompanied subjects through usual routines gathering continuous video data. Researchers developed codes to measure four behavioral domains observed on video. This paper describes the psychometric characteristics to be considered in the development of observational approaches. It also highlights differences between behaviors performed in usual environments and neuropsychological constructs. The authors demonstrate that everyday behaviors that have been shown to correspond to neurocognitive skills in a pilot feasibility study can be identified and rated. They further suggest that observational methods could provide novel strategies for linking research findings and clinical concerns.

By giving £2, you could make a big difference.

Many nurses and midwives throughout history have made a difference, and Mary Seacole was one such person. However, as a profession we lag behind our medical colleagues in remembering the historical giants on whose shoulders we stand.

Advice for leaders.

THE CATASTROPHIC collapse in standards of care and management at Mid Staffordshire NHS foundation Trust, highlighted most recently in the inquiry by Robert francis QC, published in february, has shocked the country.

Nongastric H,K-ATPase: structure and functional properties.

Nongastric H,K-ATPases whose catalytic subunits (AL1) encoded by human ATP1AL1 and homologous animal genes comprise the third distinct group within the X,K-ATPase family. No unique nongastric beta has been identified. Precise in situ colocalization and strong association of AL1 with beta1 of Na,K-ATPase was detected in apical membranes of rodent prostate epithelium. In this tissue, beta1NK serves as an authentic subunit of both the Na,K- and nongastric H,K-pumps. Upon expression in Xenopus oocytes the human AL1 can assemble with beta1NK, and more efficiently with gastric betaHK, into functional H,K-pumps. Both AL1/beta complexes exhibit a similar K-affinity, and their K-transport depends on intra- and extracellular Na. These data provide new evidence that nongastric H,K-ATPase can perform Na/K-exchange, and indicate that beta does not significantly affect this ion-pump function. Analysis of human nongastric H,K-ATPase expressed in Sf-21 insect cells revealed that AL1/betaHK exhibits substantial enzymatic activities in K-free medium and K stimulates, but Na has inhibitory effect on ATP hydrolysis. Thus, although the nongastric H,K-ATPase can function as Na/K exchanger, its reaction mechanism is different from that of the Na,K-ATPase. Human nongastric H,K-ATPase is highly sensitive to bufalin, digoxin, and digitoxin, but almost resistant to digoxigenin and ouabagenin.