Detection and genetic characterization of orf virus from sheep and goats in Nigeria.

Orf is a contagious, viral epitheliotropic disease of small ruminants. We investigated the molecular epidemiology of orf virus (ORFV) in breeds of small ruminants to determine the evolutionary diversity in Nigeria. Out of 54 small ruminants screened, the number of animals that were positive for ORFV in the three locations were 25. The distribution of positive animals by location were FCT 45.0% (n = 9/20), Oyo State 42.9% (6/14), and Plateau State 50.0% (n = 10/20). ORFV sequences from this study clustered with viruses detected in Taiwan, Iran, USA, and France. Our findings highlight the risk of transmission across geographic boundaries in Nigeria and West Africa, and reinforces the need for increased surveillance to prevent and control spread. Comprehensive characterization of ORFV in small ruminants as well as in humans in Nigeria is required to better elucidate the epidemiological dynamics and the virus evolution.

Serosurvey on Sheep Unravel Circulation of Rift Valley Fever Virus in Nigeria.

Rift Valley fever is an arboviral zoonoses causing severe morbidity and mortality among humans and animals in many African countries. A cross-sectional study in populations of sheep reared around the Gidan-Waya Forest Reserve located in Jema'a LGA of Kaduna State, Nigeria to determine the serological evidence of exposure to Rift Valley fever virus (RVFV) using a commercial competitive enzyme-linked immunosorbent assay. Of the 200 sheep sampled, 9 (4.5%; 95 CI 2.23-8.33) were positive for antibodies to the RVFV. The detection of antibodies suggests a covert circulation among the sheep and may be indicative of a subclinical infection.

Influence of levels of supplementary concentrate mixture on lactation performance of Red Sokoto does and the pre-weaning growth rate of their kids.

Twenty pregnant Red Sokoto goats (liveweight, 28 ± 1.30 kg) were used in a completely randomized design to determine the effect of varying levels of concentrate on lactation performance. The concentrate, which contained 4% palm oil, was fed at levels of 1.0, 1.5, 2.0 and 2.5% of body weight of the does in addition to a basal diet of Digitaria smutsii hay offered ad libitum. The corresponding dietary treatments were designated as 1.0%C, 1.5%C, 2.0%C and 2.5%C, respectively. The goats were balanced for parity and randomly allocated to give five animals per treatment, and stall-fed individually. The intake of dry matter and daily milk production linearly and quadratically increased (P<0.05) to the levels of concentrate supplementation. Increase in level of concentrate mixture supplementation affected (P<0.05) milk fat content and milk fat yield, but not other milk constituents. Persistency of milk production was numerically higher at higher levels of concentrate supplementation. Whereas 1.0%C, 1.5%C and 2.0%C could not prevent weight loss in the does, the 2.5%C significantly (P<0.05) promoted average daily gain (11.11 g/head/day) during lactation. The dam milk yield significantly (P<0.01) accounted for 61% of variation in kids pre-weaning average daily gain (ADG). It is concluded that concentrate mixture containing 4% palm oil can be fed at 2.5% of body weight without adverse effect on total dry matter intake, while enhancing postpartum weight gains, higher milk yield, persistency of milk production, pre-weaning growth of kids in Red Sokoto goats.

In vitro screening of two Nigerian medicinal plants (Vernonia amygdalina and Annona senegalensis) for anthelmintic activity.

Vernonia amygdalina and Annona senegalensis, two plants used by local/smallholder livestock farmers in Nigeria as anthelmintic were screened for in vitro anthelmintic activity using Haemonchus contortus eggs. The extract of V. amygdalina did not show any significant activity at concentrations up to 11.2mg/ml. The extract of A. senegalensis showed significant (P<0.001) reduction in egg hatch at a concentration of 7.1mg/ml. The in vitro fecal culture of eggs with A. senegalensis showed significant (P<0.001) reductions in larval recovery with increasing concentrations from 1 to 10% ((w/w) in fecal culture) when whole ground plant material was used. Although traditional veterinary healers and local livestock farmers claim both plants are effective as anthelmintics, our result indicate that only A. senegalensis showed promising anthelmintic activity especially with use of ground whole plant materials as used by some farmers.