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1.
Microbiol Resour Announc ; 10(4)2021 Jan 28.
Artigo em Inglês | MEDLINE | ID: mdl-33509992

RESUMO

Acholeplasma laidlawii can be isolated from cattle environments and different body sites of bovines. It is still under evaluation if A. laidlawii acts as a primary pathogen. Here, we present the whole-genome sequence of A. laidlawii isolated from the conjunctiva of a heifer with infectious bovine keratoconjunctivitis.

2.
Cell Microbiol ; 18(12): 1751-1762, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27166588

RESUMO

Neutrophil granulocytes are paramount to innate responses as major effectors of acute inflammation. Among the various strategies enacted by neutrophils to eliminate microbes NETosis is a novel distinct antimicrobial activity in which an interlacement of chromatin fibres rich in granule-derived antimicrobial peptides and enzymes is extruded (NETs, neutrophils extracellular traps ). NETs contribute to the pathogenesis of acute and chronic inflammatory disorders. The interactions of mycoplasmas and innate immune cells, in particular neutrophil granulocytes, are poorly defined. Here, we describe NET formation in vivo in the mammary gland and milk of sheep naturally infected by Mycoplasma agalactiae. Also, we assess the contribution of liposoluble proteins, the most abundant component of the Mycoplasma membrane, in inducing NETosis. We demonstrate that Mycoplasma liposoluble proteins induce NET release at levels comparable to what observed with other stimuli, such as lipopolysaccharides and phorbol 12-myristate 13-acetate. Stimulation of neutrophils with synthetic diacylated lipopeptides based on the M. agalactiae P48, P80, and MAG_1000 proteins, combined in a mix or used individually, suggests that NETosis might not be dependent on a specific lipopeptide sequence. Also, NETosis is partially abolished when TLR2 is blocked with specific antibodies. The results presented in this work provide evidences for the mechanisms underlying NET activation in mycoplasma infections, and on their contribution to pathogenesis of mycoplasmosis.


Assuntos
Proteínas de Bactérias/farmacologia , Armadilhas Extracelulares/química , Lipoproteínas/farmacologia , Glândulas Mamárias Animais/imunologia , Mycoplasma agalactiae/química , Neutrófilos/efeitos dos fármacos , Animais , Anticorpos Neutralizantes/farmacologia , Proteínas de Bactérias/síntese química , Membrana Celular/química , Membrana Celular/imunologia , Armadilhas Extracelulares/imunologia , Armadilhas Extracelulares/metabolismo , Feminino , Expressão Gênica , Lipopolissacarídeos/farmacologia , Lipoproteínas/síntese química , Glândulas Mamárias Animais/efeitos dos fármacos , Glândulas Mamárias Animais/microbiologia , Leite/imunologia , Leite/microbiologia , Mycoplasma agalactiae/imunologia , Ativação de Neutrófilo/efeitos dos fármacos , Neutrófilos/imunologia , Neutrófilos/microbiologia , Cultura Primária de Células , Ovinos , Acetato de Tetradecanoilforbol/farmacologia , Receptor 2 Toll-Like/antagonistas & inibidores , Receptor 2 Toll-Like/genética , Receptor 2 Toll-Like/imunologia
3.
BMC Cancer ; 10: 156, 2010 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-20412586

RESUMO

BACKGROUND: Breast cancer is the most frequently diagnosed cancer in women. Intraepithelial lesions (IELs), such as usual ductal hyperplasia (UH), atypical ductal hyperplasia (ADH), and ductal carcinoma in situ (DCIS) are risk factors that predict a woman's chance of developing invasive breast cancer. Therefore, a comparative study that establishes an animal model of pre-invasive lesions is needed for the development of preventative measures and effective treatment for both mammary IELs and tumors. The purpose of this study was to characterize the histologic and molecular features of feline mammary IELs and compare them with those in women. METHODS: Formalin-fixed, paraffin-embedded specimens (n = 205) from 203 female cats with clinical mammary disease were retrieved from the archives of the Purdue University Animal Disease Diagnostic Laboratory and Veterinary Teaching Hospital (West Lafayette, IN), and the Department of Pathology and Veterinary Clinic, School of Veterinary Medicine (Sassari, Italy). Histologic sections, stained with hematoxylin and eosin (HE), were evaluated for the presence of IELs in tissue adjacent to excised mammary tumors. Lesions were compared to those of humans. Immunohistochemistry for estrogen receptor (ER-alpha), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER-2/neu) and Ki-67 was performed in IELs and adjacent tumor tissues. RESULTS: Intraepithelial lesions were found in 57 of 203 (28%) feline mammary specimens and were categorized as UH (27%), ADH (29%), and DCIS (44%). Most IELs with atypia (ADH and DCIS) were associated with mammary cancer (91%), whereas UH was associated with benign lesions in 53% of cases. Feline IELs were remarkably similar to human IELs. No ER or PR immunoreactivity was detected in intermediate-grade or high-grade DCIS or their associated malignant tumors. HER-2 protein overexpression was found in 27% of IELs. CONCLUSION: The remarkable similarity of feline mammary IELs to those of humans, with the tendency to lose hormone receptor expression in atypical IELs, supports the cat as a possible model to study ER- and PR-negative breast lesions.


Assuntos
Neoplasias da Mama/patologia , Modelos Animais de Doenças , Receptor alfa de Estrogênio/biossíntese , Neoplasias Mamárias Experimentais/patologia , Receptores de Progesterona/biossíntese , Animais , Neoplasias da Mama/metabolismo , Carcinoma in Situ/metabolismo , Carcinoma in Situ/patologia , Carcinoma Ductal de Mama/metabolismo , Carcinoma Ductal de Mama/patologia , Gatos , Receptor alfa de Estrogênio/deficiência , Feminino , Humanos , Imuno-Histoquímica , Neoplasias Mamárias Experimentais/metabolismo , Receptor ErbB-2/biossíntese , Receptores de Progesterona/deficiência
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