Assessing the Landscape in Medical Education Literature in Medical Oncology: A Scoping Review.

PURPOSE: Medical oncology and medical education (ME) have both expanded exponentially over the past 50 years; thus, it is important to understand the current status of postgraduate medical oncology education and develop ways to advance this field. This study undertakes a scoping review of ME literature in medical oncology to inform future scholarship in this area. METHODS: MEDLINE, Embase, ERIC, and Web of Science were searched to find peer-reviewed English language articles on postgraduate ME in medical oncology published from 2009 to 2020. Established scoping review methodologies were used in study design; articles were classified by specialty, learner training level, region of authorship, institution type, year of publication, journal type, study methodology, and research topic. Curriculum intervention, scholarship, and domain(s) of physician competency were also assessed. The results were interpreted using descriptive statistics and collated using predetermined conceptual frameworks. RESULTS: A total of 2,959 references were initially found across four databases. After title and abstract screening, 305 articles remained; after full-text review, 144 articles were included in final analysis. Postgraduate medical oncology education research is increasing, with the majority of articles published in North America. Quantitative studies were most common, primarily survey approaches. For physician competencies, professionalism and medical expertise comprised the large majority of article focuses, whereas very few articles addressed leadership or health advocacy. Curriculum development, professional development, and communication skills were dominant research themes while no articles discussed teacher training. CONCLUSION: Although areas such as professionalism and communication skills are well-studied, medical oncology ME research is lacking in leadership, health advocacy, and teacher training. This study provides valuable guidance for future ME research in medical oncology and establishes a benchmark to examine changes in educational scholarship over time.

Long-lasting alterations to DNA methylation and ncRNAs could underlie the effects of fetal alcohol exposure in mice.

Fetal alcohol spectrum disorders (FASDs) are characterized by life-long changes in gene expression, neurodevelopment and behavior. What mechanisms initiate and maintain these changes are not known, but current research suggests a role for alcohol-induced epigenetic changes. In this study we assessed alterations to adult mouse brain tissue by assaying DNA cytosine methylation and small noncoding RNA (ncRNA) expression, specifically the microRNA (miRNA) and small nucleolar RNA (snoRNA) subtypes. We found long-lasting alterations in DNA methylation as a result of fetal alcohol exposure, specifically in the imprinted regions of the genome harboring ncRNAs and sequences interacting with regulatory proteins. A large number of major nodes from the identified networks, such as Pten signaling, contained transcriptional repressor CTCF-binding sites in their promoters, illustrating the functional consequences of alcohol-induced changes to DNA methylation. Next, we assessed ncRNA expression using two independent array platforms and quantitative PCR. The results identified 34 genes that are targeted by the deregulated miRNAs. Of these, four (Pten, Nmnat1, Slitrk2 and Otx2) were viewed as being crucial in the context of FASDs given their roles in the brain. Furthermore, ≈ 20% of the altered ncRNAs mapped to three imprinted regions (Snrpn-Ube3a, Dlk1-Dio3 and Sfmbt2) that showed differential methylation and have been previously implicated in neurodevelopmental disorders. The findings of this study help to expand on the mechanisms behind the long-lasting changes in the brain transcriptome of FASD individuals. The observed changes could contribute to the initiation and maintenance of the long-lasting effect of alcohol.

Radiation exposure differentially affects songbird 8-hydroxy-2'-deoxyguanosine plasma profiles: ionizing radiation damage response in songbirds.

The importance of understanding the effects of radiation exposure on wildlife is a critical responsibility of our stewardship of nuclear energy production. We tested the hypothesis that songbirds respond to exogenous radiation exposure with changes in plasma 8-hydroxy-2'-deoxyguanosine (8-OH-dG). We exposed two species of songbirds, house sparrows (Passer domesticus; n = 12) and song sparrows (Melospiza melodia; n = 12), to one of four acute whole-body radiation treatments: 75, 150, 300, or 600 mGy. We measured DNA damage by proxy as 8-OH-dG levels in the plasma at 0 hr (baseline), 36 hr, and 7 days post radiation. For both species, at all radiation treatments, 8-OH-dG levels increased significantly 36 hr following radiation exposure. However, songbird species differed significantly in response to treatment across time and between treatment groups. Song sparrows showed no significant changes in 8-OH-dG levels between 36 hr and Day 7. In contrast, house sparrows exposed to 300 and 600 mGy had significantly increased 8-OH-dG levels at Day 7 compared with 36 hr. This study demonstrates that in a controlled experiment, in isolation from other sources of genotoxicity, radiation exposure significantly affects songbirds. Our results suggest future research examining the effects of radiation on songbirds must consider using multiple species to assess the biological effects of radiation, as different species can show strikingly different responses to radiation dosage across time.