RESUMO
The sera of RSV tumour-bearing rats, hamsters and monkeys were tested for the presence of antibodies to RSV proteins. Complement fixation test and radioimmunoprecipitation followed by SDS-polyacrylamide gel electrophoresis were used for detection of antibodies to the products of the gag, env and src genes of RSV. Antibodies to the env and gag gene products were found in sera of tumour-bearing monkeys. Hamster sera contained antibodies to the gag gene products only. No antibodies were detected in sera of tumour-bearing rats. The differences in immunogenicity of RSV gene products in various hosts are discussed.
Assuntos
Anticorpos Antivirais/análise , Vírus do Sarcoma Aviário/imunologia , Sarcoma Aviário/imunologia , Proteínas Virais/imunologia , Animais , Testes de Fixação de Complemento , Cricetinae , Eletroforese em Gel de Poliacrilamida , Macaca mulatta , Mesocricetus , Ratos , Sarcoma Experimental/imunologiaRESUMO
The Carr-Zilber strain of Rous sarcoma virus is highly oncogenic for adult monkeys of the species Macaca mulata. In the present study, we examined some characteristics of tumor growth when the tumor either regressed or progressed until it killed its host. We followed the kinetics of antibody formation to antigens coded by CZ-RSV in animals with a different course of tumor disease. The sera of animals tested contained virus-neutralizing antibodies, in addition to gs antibodies. The results are discussed with regard to the possible virus-productive type of interaction between RSV and the monkey cell.
