Retrieval uncertainty and consistency of Suomi-NPP VIIRS Deep Blue and Dark Target aerosol products under diverse aerosol loading scenarios over South Asia.

Retrieval accuracy and stability of two operational aerosol retrieval algorithms, Deep Blue (DB) and Dark Target (DT), applied on Visible Infrared Imaging Radiometer Suite (VIIRS) on-board Suomi National Polar-orbiting Partnership (S-NPP) satellite were evaluated over South Asia. The region is reported to be highly challenging to accurate estimation of satellite-based aerosol optical properties due to variations in surface reflectance, complex aerosol system and regional meteorology. Performance of both algorithms were initially evaluated by comparing their ability to retrieve aerosol signal over the complex geographical region under specific air pollution emission scenario. Thereafter, retrieval accuracy was investigated against 10 AERONET sites across South Asia, selected based on their geography and predominance aerosol types, from year 2012-2021. Geo-spatial analysis indicates DB to efficiently retrieve fine aerosol features over bright arid surfaces, and for smoke/dust dominating events whereas DT was better to identify small fire events under dark vegetated surface. Both algorithms however, indicate unsatisfactory retrieval accuracy against AERONET having 56-59% of valid retrievals with high RMSE (0.30-0.33) and bias. Overall, DB slightly underpredicted AOD with -0.02 mean bias (MB) whereas DT overpredicted AOD (MB: 0.13), with seasonality in their retrieval efficiency against AERONET. Time-series analysis indicates stability in retrieving AOD and match-up number for both algorithms. Retrieval bias of DB and DT AOD against AERONET AOD under diverse aerosol loading, aerosol size, scattering/absorbing aerosol, and surface vegetation coverage scenarios revealed DT to be more influenced by these conditions. Error analysis indicates at low AOD (≤0.2), accuracy of both DB and DT were subject to underlying vegetation coverage. At AOD>0.2, DB performed well in retrieving coarse aerosols whereas DT was superior when fine aerosols dominated. Overall, accuracy of both VIIRS algorithms require further refinement to continue MODIS AOD legacy over South Asia.

Three-dimensional nature of summertime aerosols over South Asia.

Three-dimensional (temporal-spatial-vertical) climatology of South Asian summertime (MAMJ, 2010-2019) aerosols and aerosol sub-types was explored using multiple high-resolution satellite-based observations and reanalysis dataset. Vertical stratification of aerosol layer and aerosol sub-types was identified using observation from space-borne lidar. Aerosol optical depth (AOD) was particularly high across the Indo-Gangetic Plain (IGP; AOD ± SD: 0.56 ± 0.12) and over eastern coast of India (AOD: 0.6-0.8), with prevalence of heterogeneous aerosol sub-types having strong spatial gradient. Clearly, aerosols over north-western arid part were highly absorbing (Ultra-violet Aerosol Index, UVAI > 0.80) and coarse (Ångström exponent, AE < 0.8), with an indication of desert/-mineral dust aerosols. In contrast, fine and moderate to non-absorbing aerosols (UVAI: 0.20-0.50) dominate from central to lower IGP, including in Bangladesh, with signature of anthropogenic emissions. Prevailing aerosols over twelve South Asian cities were classified into six aerosol sub-types constraining their particle size and UV-absorbing potential. Overall, mineral dust, smoke and urban aerosols were the three major aerosol sub-types that prevail across South Asia during summer. In particular, 58-70 % of retrieval days over Karachi and Multan were dust dominated; 57-64 % days were dust or urban aerosols dominated over Lahore, Delhi, Kanpur and Varanasi, and 56-77 % days were smoke or urban aerosols dominated over Dhaka, Kathmandu, Chennai, Mumbai, Colombo and Nagpur. Prevailing aerosols were vertically stratified as 50-70 % of total AOD was retrieved <2 km from the surface except in few cities where 70-80 % of AOD was retrieved <3 km height. Mineral dust and/or urban aerosols emerged as the most abundant aerosol types near the surface (<1 km) in all the cities except in Chennai, with their abundance remained as a function of emission sources and geographical location.

Management of altered metabolic activity in Drosophila model of Huntington's disease by curcumin.

Huntington's disease (HD) is a devastating polyglutamine disorder characterized by extensive neurodegeneration and metabolic abnormalities at systemic, cellular and intracellular levels. Metabolic alterations in HD manifest as abnormal body weight, dysregulated biomolecule levels, impaired adipocyte functions, and defective energy state which exacerbate disease progression and pose acute threat to the health of challenged individuals in form of insulin resistance, cardiovascular disease, and energy crisis. To colossally mitigate disease symptoms, we tested the efficacy of curcumin in Drosophila model of HD. Curcumin is the bioactive component of turmeric (Curcuma longa Linn), well-known for its ability to modulate metabolic activities. We found that curcumin effectively managed abnormal body weight, dysregulated lipid content, and carbohydrate level in HD flies. In addition, curcumin administration lowered elevated reactive-oxygen-species levels in adult adipose tissue of diseased flies, and improved survival and locomotor function in HD flies at advanced disease stage. Altogether, these findings clearly suggest that curcumin efficiently attenuates metabolic derangements in HD flies and can prove beneficial in alleviating the complexities associated with HD.

Peripheral Expression of Mutant Huntingtin is a Critical Determinant of Weight Loss and Metabolic Disturbances in Huntington's Disease.

Deteriorating weight loss in patients with Huntington's disease (HD) is a complicated peripheral manifestation and the cause remains poorly understood. Studies suggest that body weight strongly influences the clinical progression rate of HD and thereby offers a valuable target for therapeutic interventions. Mutant huntingtin (mHTT) is ubiquitously expressed and could induce toxicity by directly acting in the peripheral tissues. We investigated the effects of selective expression of mHTT exon1 in fat body (FB; functionally equivalent to human adipose tissue and liver) using transgenic Drosophila. We find that FB-autonomous expression of mHTT exon1 is intrinsically toxic and causes chronic weight loss in the flies despite progressive hyperphagia, and early adult death. Moreover, flies exhibit loss of intracellular lipid stores, and decline in the systemic levels of lipids and carbohydrates which aggravates over time, representing metabolic defects. At the cellular level, besides impairment, cell death also occurs with the formation of mHTT aggregates in the FB. These findings indicate that FB-autonomous expression of mHTT alone is sufficient to cause metabolic abnormalities and emaciation in vivo without any neurodegenerative cues.

Altered lipid metabolism in Drosophila model of Huntington's disease.

Huntington's disease (HD) is late-onset, progressive neurodegenerative disorder caused by expansion of polyglutamine (polyQ) repeat within Huntingtin (Htt) protein. In HD patients, energy-related manifestations such as modulation of weight during entire course of disease with energy deficit at terminal stage have been reported, however, underlying reason remains elusive till date. Lipids, carbohydrate and protein constitute a predominant fraction of body's energy reservoir and perturbation in their homeostasis may influence weight. To discern role of these energy molecules in weight alteration, we quantified them in an in vivo transgenic Drosophila model of HD. We document that diseased flies exhibit change in weight due to an altered lipid metabolism, as evident from considerably high lipid levels at the time of disease onset followed by a pathologic decline at end-stage. An alteration in intracellular lipid droplet size suggested altered cellular lipid turnover. Furthermore, diseased flies displayed substantial changes in carbohydrate and protein content. Interestingly, alteration in weight and lipid levels are independent of the feeding pattern in diseased condition and exhibit weak correlation with insulin-like peptide or adipokinetic hormone producing cells. We propose that therapeutic intervention aimed at restoring lipid levels and associated metabolic pathways may improve longevity and quality of patient's life.