Health Beneficial Phytochemicals in Dioscorea caucasica Lipsky Leaves and Tubers and Their Inhibitory Effects on Physiologically Important Enzymes.

Dioscorea caucasica Lipsky is a tertiary relict endemic plant naturally growing in the western part of the trans-Caucasus regions; it has adapted and successfully grows in the temperate region of the Baltic countries. Information about its phytochemical composition and bioactivities is rather scarce. This study reports the results of the identification of 41 compounds in D. caucasica leaf and tuber hydroethanolic extracts using UPLC-QTOF/MS. Organic acids were found in both extracts; hydroxycinnamates and flavonoids were the main phytochemicals in the leaves, while steroidal glycosides, fatty acids (mainly hydroxylated) and carbohydrates were found in the tubers. Leaf extracts inhibited enzymes in a dose-dependent manner and were remarkably stronger inhibitors of physiologically important enzymes, namely α-amylase (48.6% at 480 µg/mL), α-glucosidase (IC50 = 41.99 and 47.95 µg/mL with and without 0.1 M Na2CO3), acetylcholinesterase (45.85% at 100 µg/mL) and angiotensin-converting enzyme (IC50 = 829.7 µg/mL), most likely due to the presence of some quantified polyphenolic antioxidants. The mode of inhibition of α-glucosidase and acetylcholinesterase was assessed via kinetic studies based on Lineweaver-Burk inhibition plots. Leaf and tuber extracts acted as mixed-type and competitive inhibitors of α-glucosidase, respectively; the leaf extract demonstrated an uncompetitive inhibition mode of acetylcholinesterase. It is expected that this new knowledge of D. caucasica will serve for its valorization in developing new health beneficial ingredients for functional foods and nutraceuticals.

Dioscorea spp.: Comprehensive Review of Antioxidant Properties and Their Relation to Phytochemicals and Health Benefits.

Dioscorea, consisting of over 600 species, is the most important genus in the Dioscoreaceae family; however, the practically used plants, which are commonly called yam, are restricted to a remarkably smaller number of species. Numerous studies have reported the high nutritional value of yam, particularly as an alternative source of starch and some important micronutrients. Several Dioscorea species are widely used for various medicinal purposes as well. In many studies, the bioactivities and health benefits of Dioscorea extracts and other preparations have been related to the presence of phytochemicals, which possess antioxidant properties; they are related mainly to radical-scavenging capacity in chemical assays and positive effects on the endogenous antioxidant system in cell-based and in vivo assays. Considering the increasing number of publications on this topic and the absence of comprehensive and focused review papers on antioxidant potential, this article summarizes the results of studies on the antioxidant properties of Dioscorea spp. and their relation to phytochemicals and health benefits. A comprehensive survey of the published articles has revealed that the majority of studies have been performed with plant tubers (rhizomes, roots), while reports on leaves are rather scarce. In general, leaf extracts demonstrated stronger antioxidant potential than tuber preparations. This may be related to the differences in phytochemical composition: saponins, phenanthrenes and, for some pigment-rich species (purple yams), anthocyanins are important constituents in tubers, while phenolic acids and flavonoids are characteristic phytochemicals in the leaves. The review may assist in explaining ethnopharmacological knowledge on the health benefits of Dioscorea plants and their preparations; moreover, it may foster further studies of poorly investigated species, as well as their wider application in developing new functional foods and nutraceuticals.

Genotoxic properties of Betonica officinalis, Gratiola officinalis, Vincetoxicum luteum and Vincetoxicum hirundinaria extracts.

Genotoxicity of B. officinalis, G. officinalis, V. luteum and V. hirundinaria extracts, which demonstrated strong antioxidant capacity, was tested using chromosome aberration, sister chromatid exchange (SCE), cytokinesis-block micronucleus and alkaline single-cell gel electrophoresis (comet) assays in human lymphocytes in vitro and Ames Salmonella/microsome test. All tested extracts were not mutagenic in S. typhimurium strains TA98 and TA100 with and without metabolic activation and did not induce chromosome aberrations in human lymphocytes in vitro. Extract from G. officinalis was the only one, which induced significant increase in micronuclei, indicating possible aneugenic effect. All investigated plant extracts induced DNA damage evaluated by the comet assay, while B. officinalis and V. luteum extracts induced slight increase in SCE values. The determined variation in response might be due to the plant extract tested and donor susceptibility.