One-stage Full Mouth Disinfection Using 20% Propolis Hydroalcoholic Solution: A Clinico-microbiologic Study.

BACKGROUND: Propolis is a resinous substance produced by honeybees which has many therapeutic properties because of its unique composition. It has been widely used since many years for different medicinal purposes. AIM: The aim of this study was to investigate the effects of one-stage full mouth disinfection (OSFMD) using 20% propolis hydroalcoholic solution in chronic periodontitis patients. MATERIALS AND METHODS: Thirty patients diagnosed with chronic periodontitis and presenting three or more nonadjacent teeth with deep pockets were selected for the study. Clinical parameters including gingival index, plaque index, bleeding on probing, probing pocket depth, and clinical attachment level were recorded at baseline in all the patients followed by subgingival plaque sampling. All the thirty patients were randomly allocated into two groups; 15 patients (control group) were subjected to scaling and root planning (SRP) alone, and in remaining 15 patients (test group), SRP was done followed by OSFMD using 20% propolis hydroalcoholic solution after 24 h. All the patients were kept at periodic recall, and clinical and microbiological parameters were again taken at 4 weeks and 12 weeks. RESULTS: There was a significant improvement for all the clinical parameters, with higher probing depth reduction and attachment gain in the test group when compared to the control group. Furthermore, the microbiological counts of the periodontopathogens were found to decrease considerably more in the test group. CONCLUSION: SRP followed by OSFMD with propolis extract after 24 h was more effective than SRP alone in chronic periodontitis patients.

Comparative Evaluation of the Efficacy of Human Chorion and Amnion With Coronally Advanced Flap for Recession Coverage: A Case Series.

INTRODUCTION: An amnion-chorion membrane is a placenta-derived tissue that has been introduced recently as a guided tissue regeneration membrane in dentistry. Numerous growth factors, proteins, and stem cell reserves in amnion could help in accelerated wound healing and regeneration, whereas chorion is a rich source of various collagen and non-collagen proteins. The present six-patient observational case series compares amnion and chorion allografts for recession coverage. CASE SERIES: Six healthy adult patients presenting with 12 Miller Class I recession defects were treated surgically with a modified coronally advanced flap along with amniotic membrane (group A) or chorion (group B) for coverage of the recession defects following a split-mouth design. Clinical parameters measured at baseline, 3 months, and 6 months were width of keratinized gingiva (WKG), probing depth, recession height, clinical attachment level (CAL), and assessment of gingival biotype. Statistical analysis was done using paired and unpaired Student t test. The results showed statistically significant (P <0.01) improvements in all clinical parameters at the 3- and 6-month follow-ups in both groups. Nine of 12 treated recession defects showed 100% root coverage. The gingival biotype also showed a thick biotype in 10 sites that had an initial thin biotype. CONCLUSION: Both amnion and chorion membranes provided promising results in terms of root coverage, increased WKG, CAL gain, and thickness of the gingival biotype and can be used for periodontal plastic surgeries.

A comparative evaluation of topical and intrasulcular application of coenzyme Q10 (Perio Q™) gel in chronic periodontitis patients: A clinical study.

BACKGROUND AND OBJECTIVES: Coenzyme Q10 is a well-studied antioxidant in the medical literature, but studies regarding its efficacy in periodontal diseases are few. coenzymeoenzyme Q10 serves as an endogenous antioxidant and its increased concentration in the diseased gingiva effectively suppresses advanced periodontal inflammation. The aim of this study is to evaluate the efficacy of coenzyme Q10 (Perio Q™) as an adjunct to scaling and root planing in patients with chronic periodontitis. MATERIALS AND METHODS: A total of 18 patients were enrolled for the study. The selected subjects were treated in three different quadrants randomly. The control quadrant was treated by scaling and root planing only, while the other two test quadrants were treated by intra-pocket application of gel combined with scaling or root planing and topical applications combined with scaling and root planning, respectively. Clinical parameters such as plaque index, gingival index, gingival bleeding index and probing pocket depth were assessed at baseline and at the 2(nd) week and 4(th) weeks. The results were subjected to statistical analysis. RESULTS: There was a significant improvement in all clinical parameters in the test sites seen at the end of the 4-week period. Sites with bleeding on probing were reduced more in the test group than in the control group. CONCLUSION: Coenzyme Q10 can be said to have a beneficial effect on periodontitis when used as an adjunct to scaling and root planing.

Treatment of drug-induced gingival overgrowth by full-mouth disinfection: A non-surgical approach.

BACKGROUND: Drug-induced gingival overgrowth is a common finding in the modern era. These gingival overgrowths are usually treated by various modalities namely substitution of drugs, surgical, and non-surgical treatment. The recent concept mainly involves full-mouth scaling and root planing (the entire dentition in two visits within 24 hours, i.e., two consecutive days) followed by chair side mouth rinsing by the patient with a 0.2% chlorhexidine solution for 2 minutes and brushing the tongue of the patient with 1% chlorhexidine gel. This is followed by an additional subgingival irrigation (three times, repeated within 10 minutes) of all pockets with a 1% chlorhexidine gel. MATERIALS AND METHODS: Twenty patients between the ages of 20 and 50 years with drug-induced gingival overgrowth were treated using the full-mouth disinfection approach. The patients were evaluated at 3 months and 6 months after therapy. The data obtained for plaque index, bleeding on probing index, probing pocket depth, and gingival overgrowth scores were tabulated and compared statistically using the one sample unpaired t test. STATISTICAL ANALYSIS: Statistically significant difference (P < 0.05) was found in PI GBI, PPD, and GO score between baseline, 3 months, and 6 months. RESULTS: All clinical parameters improved significantly after therapy without the need of further surgical treatment. CONCLUSIONS: Full-mouth disinfection might be a beneficial treatment concept in patients with drug-induced gingival overgrowth, thus decreasing the need for surgical therapy.

Oratest: a new concept to test caries activity.

Caries activity tests are based on the concept of a specific odontogenic infection, the principle causative organism being streptococci mutans. Their predominance is attributed to its acidogenic and aciduric nature after a selective growth advantages over the other non- acid tolerant organisms. Many studies on caries activity are aimed at finding relevant microorganisms. Till date, the ideal method to evaluate in terms of sensitivity, specialization and reliability has not been found. Many of these caries activity tests require extensive work up time and additional equipment. Rosenberg et al. in 1989 developed Oratest, a simple, economical, non- invasive and less time-consuming test for estimating the oral microbial level. The test is simple and consists of rinsing the mouth with 10 ml of sterile milk, 3 ml of which is mixed with 0.12 ml of 0.1% methylene blue dye and observed for colour change. The present study sample consists of twenty five children with dental caries and twenty five controls, free of caries, gingivitis and other oral ailments. This study is being conducted in the department of Oral Pathology & Microbiology and is in the preliminary phase so further results are awaited.