RESUMO
Heteropolysaccharides isolated from the racemes of families Polygonaceae, Tiliaceae, and Asteraceae intensify but do not normalize completely the development of the immune response in rats subjected to immersion (water) cooling. A similar effect is caused by erythrocytes of intact rats treated by heteropolysaccharides extracorporeally. On inclusion of heteropolysaccharides into the erythrocytic stroma of intact and cooled animals normalize the development of the immune response in rats subjected to immersion cooling.
Assuntos
Adjuvantes Imunológicos/farmacologia , Asteraceae , Temperatura Baixa/efeitos adversos , Imersão/efeitos adversos , Polygonaceae , Polissacarídeos/farmacologia , Árvores , Adjuvantes Imunológicos/administração & dosagem , Animais , Portadores de Fármacos , Eritrócitos , Imunidade Celular/efeitos dos fármacos , Imunização/métodos , Imunização/estatística & dados numéricos , Extratos Vegetais/administração & dosagem , Extratos Vegetais/farmacologia , Polissacarídeos/administração & dosagem , Ratos , Ratos Wistar , Estatísticas não ParamétricasRESUMO
Beta-carotene, essential, and the heteropolysaccharide of chamomile racemes reduce the manifestation of the antioxidant status and immunological reactivity caused by air and immersion (water) cooling. The effect of the drugs is mediated by various fractions of the erythrocytes.
Assuntos
Adjuvantes Imunológicos/uso terapêutico , Ar , Reações Antígeno-Anticorpo/efeitos dos fármacos , Antioxidantes/metabolismo , Antioxidantes/uso terapêutico , Temperatura Baixa/efeitos adversos , Imersão/efeitos adversos , Animais , Avaliação Pré-Clínica de Medicamentos , Hipotermia/tratamento farmacológico , Hipotermia/imunologia , Hipotermia/metabolismo , Imunização , Ratos , Ratos WistarRESUMO
Intragastric and parenteral administration of heteropolysaccharides of Matricaria chamomilla L. is found to normalize developing of the immune response upon air cooling and enhance (but do not normalize) this process upon immersion cooling. The immunomodulating effect of the heteropolysaccharides upon cooling is attributed to initiation of immunostimulating properties of heavy erythrocytes (macrocytes), activization of immunoregulation cells of peripheral blood, and increased sensitivity of effector cells to helper signals.
Assuntos
Adjuvantes Imunológicos/farmacologia , Ar , Camomila , Temperatura Baixa/efeitos adversos , Compostos Heterocíclicos/farmacologia , Imersão/efeitos adversos , Plantas Medicinais , Polissacarídeos/farmacologia , Adjuvantes Imunológicos/administração & dosagem , Animais , Relação Dose-Resposta a Droga , Compostos Heterocíclicos/administração & dosagem , Imunidade Celular/efeitos dos fármacos , Imunização/métodos , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Polissacarídeos/administração & dosagem , Ratos , Ratos Wistar , Estatísticas não ParamétricasRESUMO
Immunosuppression caused by accumulation of antiproteolytic proteins and low-density lipoproteins and very low-density lipoproteins develops in acute renal failure (ARF) induced by HgCl2. An increase in the activity of antiproteolytic proteins occurs under the influence of factors of glass-adhering splenocytes whose elaboration is induced by heavy red blood cells modified by proteases. Lipoproteins give rise to the capacity of light red blood cells to activate the generation of suppressing cytokines by glass-adhering splenocytes. Lysozyme is an effective modulator in ARF.
Assuntos
Injúria Renal Aguda/imunologia , Adjuvantes Imunológicos/fisiologia , Tolerância Imunológica , Baço/imunologia , Animais , Ratos , Ratos WistarRESUMO
The activating effect of a M-receptor agonist carbachol on phosphatidylinositol metabolism under perfusion of the isolated rabbit heart with 32Pi was shown. An increase of the inclusion of 32P in phosphatidylinositol-4-phosphate and phosphatidylinositol-4-diphosphate was found. Simultaneously there was detected an elevation of the levels of products of hydrolysis of inositol phospholipids--inositol-1,4-diphosphate and inositol-1,4,5-triphosphate. There was noted the blocking action of carbachol on the contractile activity of the heart that manifested itself in a lowering of the systolic pressure in the left ventricle and a slowing of the contraction rate.
Assuntos
Coração/efeitos dos fármacos , Miocárdio/metabolismo , Fosfatidilinositóis/metabolismo , Receptores Muscarínicos/efeitos dos fármacos , Trifosfato de Adenosina/metabolismo , Animais , Carbacol/farmacologia , Técnicas In Vitro , Contração Miocárdica/efeitos dos fármacos , Perfusão/métodos , Radioisótopos de Fósforo , Coelhos , Receptores Muscarínicos/fisiologia , Sarcolema/efeitos dos fármacos , Sarcolema/metabolismoRESUMO
Phosphorylation of cardiac sarcolemma proteins under stimulation of M-receptors by agonist carbacholine used to stimulate phosphatidylinositide cycle, was investigated in the isolated, rabbit heart perfused with 32Pi. Carbacholine (10(-7) stimulates the polyphosphoinositide metabolism which is expressed in the activated incorporation of 32P from [gamma-32P]ATP in polyphosphoinositide as well as in the increased content of the labelled inositol trisphosphate released through phosphatidylinositol-4,5-bisphosphate break-down by phospholipase C. The diacylglycerol produced simultaneously with inositol triphosphate as a second messenger activates the protein kinase C. This was confirmed by considerable activation of phosphorylation sarcolemma proteins-substrates of protein kinase C, with Mr 94, 87, 78, 51 and 46 kDa.
