Assuntos
Herpesvirus Humano 4/genética , Neoplasias Nasofaríngeas/imunologia , Sequências Repetitivas de Ácido Nucleico , Proteínas Virais/imunologia , Diferenciação Celular , Herpesvirus Humano 4/metabolismo , Humanos , Neoplasias Nasofaríngeas/patologia , Neoplasias Nasofaríngeas/virologia , Proteínas Virais/genéticaRESUMO
A pMAL-vector-based plasmid clone with synthetic tac-promotor effectively expressing full-length terminal repeats protein 1 (TP1) of Epstein-Barr virus (EBV) in E. coli was constructed. It is important that the N-terminal region of recombinant TP1 was represented by a maltose-binding protein. The latter can be used to separate TP1 from bacterial lysate by affinity chromatography. Moreover, after treatment with the proteolytic factor Xa, full-length TP1 can be recovered in a discrete form. On the basis of the pATH tryptophan-regulated vector, several plasmid clones expressing different fragments of N- and C-terminal regions of TP1 were also constructed. This collection of recombinant proteins could be used as an important tool for obtaining corresponding antisera and for immunological mapping of the TP1 molecule.
Assuntos
Escherichia coli/genética , Herpesvirus Humano 4/metabolismo , Sequências Repetitivas de Ácido Nucleico , Proteínas Virais/genética , Clonagem Molecular , Plasmídeos , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Solubilidade , Proteínas Virais/químicaRESUMO
This is the first presentation of information on the circulation of human herpes virus type 6 (HHV-6) in the population of the mid-European part of this country. The results of the study indicate that HHV-6 is widely spread both among apparently normal people and among patients with different pathologies. The studies using immunofluorescence test showed that the percentage of HHV-6-infected subjects was practically similar among blood donors, patients with nasopharyngeal cancer, rheumatoid arthritis, hemophilia, and patients with transplanted kidney, varying from 34% to 49%. A significantly higher per cent (66%) and higher antibody titres to HHV-6 were detected in sera from patients with hemoblastoses. A high level of HHV-6 infection among patients with hemoblastoses was also confirmed by the results of immunoblotting showing a wider spectrum and higher intensity of the detectable proteins. It should be noted that the amounts of virus-specific proteins detectable by some sera showed individual variations indicating different levels of expression of viral proteins in different individuals.