Depolymerized carrageenan expresses elicitor-like activity on Mentha arvensis L. under arsenic stress: Insights into arsenic resilience and monoterpene synthesis.

Heavy metals contaminate agricultural land by limiting the productivity of crops and making them or their products unfit for consumption. Arsenic (As) is a potentially hazardous metalloid that severely impacts plants' survival. Menthol mint (Mentha arvensis L.) bears volatile compounds that are harshly exaggerated by diverse environmental factors like drought, salinity, heavy metal, temperature, photoperiod, and luminosity stresses. In this study, the phytotoxicity of As was examined in M. arvensis L. and its alleviation through the supplementation of oligomers of carrageenan. Noticeably, scanty information is available regarding the effect of irradiated carrageenan (ICA) on As-stressed plants. In order to observe the same in the case of M. arvensis L., the effect of ICA on As-treated plants was explored. The ICA concentration (foliar-applied) selected for the study was 80 mg L-1, 100 mg L-1 and 120 mg L-1, and that of As (soil-applied) was 80 mg kg-1 soil. Excess accumulation of As resulted in reduced growth, enzymatic activities, and yield and quality parameters of M. arvensis L. under As toxicity. However, the foliage application of ICA strengthens the antioxidant machinery and other physiological and oxidative stress biomarkers of the plant by facilitating the activity of superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), and proline, and, therefore aids in alleviating the toxicity generated by As. Nevertheless, ICA supplementation proves beneficial in enhancing the monoterpene synthesis (essential oil production and its active constituents) of M. arvensis L. by maintaining a steady-state equilibrium between reactive oxygen species (ROS) production and its scavenging process.

Melatonin supplementation combats nickel-induced phytotoxicity in Trigonella foenum-graecum L. plants through metal accumulation reduction, upregulation of NO generation, antioxidant defence machinery and secondary metabolites.

Nickel (Ni) at a toxic level (80 mg kg-1 of soil) adversely affects the crop performance of fenugreek (Trigonella foenum-graecum L.). Melatonin (MEL), a potent plant growth regulator, is ascribed to offer promising roles in heavy metal stress alleviation. In this study, different doses viz. 0, 25, 50, 75 and 100 µM of MEL were administered to plants through foliage under normal and Ni-stress conditions. The experiment unveiled positive roles of MEL in enhancing root-shoot lengths, fresh-dry weights, seed yield and restoring photosynthetic efficiency assessed in terms of higher Fv/Fm, YII, qP, and lower NPQ values in plants exposed to Ni (80 mg kg-1). MEL supplementation (at 75 µM) effectively restricted Ni accumulation and regulated oxidative stress via modulation of MDA, O2-, H2O2 and NO generation, most prominently. Besides, MEL at 75 µM more conspicuously perked up the activities of antioxidant enzymes like SOD, POX, CAT and APX by 15.7, 20.0, 14.5 and 16.5% higher than the Ni-exposed plants for effective ROS scavenging. Likewise, MEL at 75 µM also efficiently counteracted Ni-generated osmotic stress, through an upscaled accumulation of proline (19.6%) along with the enhancement in the concentration of total phenols (13.6%), total tannins (11.2%), total flavonoids (25.5%) and total alkaloids (19.2%) in plant's leaves. Furthermore, under 80 mg kg-1 Ni stress, MEL at 75 µM improved the seed's trigonelline content by 40.1% higher compared to Ni-disturbed plants, upgrading the pharmacological actions of the plant. Thus, the present study deciphers the envisaged roles of MEL in the alleviation of Ni stress in plants to enhance overall crop productivity.

Silicon dioxide nanoparticles suppress copper toxicity in Mentha arvensis L. by adjusting ROS homeostasis and antioxidant defense system and improving essential oil production.

Copper (Cu) is an essential micronutrient for plants; however, the excessive accumulation of Cu due to various anthropogenic activities generates progressive pollution of agricultural land and that causes a major constraint for crop production. Excess Cu (80 mg kg-1) in the soil diminished growth and biomass, photosynthetic efficiency and essential oil (EO) content in Mentha arvensis L., while amplifying the antioxidant enzyme's function and reactive oxygen species (ROS) production. Therefore, there is a pressing need to explore effective approaches to overcome Cu toxicity in M. arvensis plants. Thus, the present study unveils the potential of foliar supplementation of two distinct forms of silicon dioxide nanoparticles (SiO2 NPs) i.e., Aerosil 200F and Aerosil 300 to confer Cu stress tolerance attributes to M. arvensis. The experiment demonstrated that applied forms of SiO2 NPs (120 mg L-1), enhanced plants' growth and augmented the photosynthetic efficiency along with the activities of CA (carbonic anhydrase) and NR (nitrate reductase), however, the effects were more accentuated by Aerosil 200F application. Supplementation of SiO2 NPs also exhibited a beneficial effect on the antioxidant machinery of Cu-disturbed plants by raising the level of proline and total phenol as well as the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX) and glutathione reductase (GR), thereby lowering ROS and electrolytic leakage (EL). Interestingly, SiO2 NPs supplementation upscaled EO production in Cu-stressed plants with more pronounced effects received in the case of Aerosil 200F over Aerosil 300. We concluded that the nano form (Aerosil 200F) of SiO2 proved to be the best in improving the Cu-stress tolerance in plants.

Nitric oxide induces antioxidant machinery, PSII functioning and artemisinin biosynthesis in Artemisia annua under cadmium stress.

Soil contamination by heavy metals poses a significant environmental challenge, as the practical implementation of existing remediation technologies in the field has encountered numerous obstacles. This has necessitated the requirement of finding alternate solutions to reduce the harm caused to plants. In this study, nitric oxide (NO) was investigated for its potential to reduce cadmium (Cd) toxicity in A. annua plants. Although NO plays a vital role in the growth and development of plants, information on its role in reducing abiotic stress in plants is limited. A. annua plants were exposed to 20 and 40 mg/kg Cd regardless of the addition of exogenous sodium nitroprusside (SNP), a NO donor, at 200 µM concentration. Results showed that SNP treatment improved plant growth, photosynthesis, chlorophyll fluorescence, pigment content, and artemisinin production while reducing Cd accumulation and improving membrane stability in A. annua during Cd stress. The results demonstrated that NO can effectively reverse Cd-induced damage in A. annua by modulating the antioxidant system, maintaining redox homeostasis, and improving photosynthetic performance and different fluorescence parameters such as Fv/Fm, ФPSII, and ETR. The supplementation of SNP caused a substantial improvement in chloroplast ultrastructure, stomatal behavior, and different attributes relate to glandular secretory trichomes, which in turn increased artemisinin production; 14.11 % in plants exposed to Cd stress of 20 mg/kg. Our findings highlight that NO could be useful in mediating the repair of Cd-induced damage to A. annua, and suggest that it may play a critical role in plant signaling networks, improving plant adaptability to Cd stress. The results have important implications for developing new strategies to mitigate the negative impacts of environmental contaminants on plant health, and ultimately, the ecosystem.

Engineered nanoparticles in plant growth: Phytotoxicity concerns and the strategies for their attenuation.

In the agricultural sector, the use of engineered nanoparticles (ENPs) has been acclaimed as the next big thing for sustaining and increasing crop productivity. A vast amount of literature is available regarding the growth-promoting attributes of different ENPs. In this context, it has been emphasized that the ENPs can bolster vegetative growth, leaf development, and seed setting and also help in mitigating the effects of abiotic and biotic stresses. At the same time, there have been a lot of speculations and concerns regarding the phytotoxicity of ENPs off-late. In this connection, many research articles have presented the negative effects of ENPs on plant systems. These studies have highlighted that almost all the ENPs impart a certain degree of phytotoxicity in terms of reduction in growth, biomass, impairment of photosynthesis, oxidative status of plant cells, etc. Mostly, the ENPs based on metal or metal oxides (Cd, Cr, Pb, Ag, Ce, etc.) and nonmetals (C) that are introduced into the environment are known to incite inhibitory effects. However, the phytotoxicity of ENPs are known to be determined mostly by the chemical nature of the element, size, surface charge, coating molecules, and abiotic factors like pH and light. This review article, therefore, elucidates the phytotoxic properties of different ENPs and the plant responses induced at the molecular level subjected to nanoparticle exposure. Moreover, the article highlights the probable strategies that may be adopted for the suppression of the phytotoxicity of ENPs to ensure the safe and sustainable application of ENPs in crop fields.

Exogenous abscisic acid fine-tunes heavy metal accumulation and plant's antioxidant defence mechanism to optimize crop performance and secondary metabolite production in Trigonella foenum-graecum L. under nickel stress.

Nickel (Ni) contamination of farming soil has become currently a recurring global menace to agriculture crop productivity. The purpose of the present study was to investigate the putative contributions of abscisic acid (ABA) to extemporize Ni tolerance in Trigonella foenum-graecum L. (fenugreek) plants. The outcomes of this study exposed that exogenous supplementation of ABA at 10, 20, 40 and 80 µM considerably enhanced the growth and physiological attributes of fenugreek under 80 mg Ni kg-1 soil, however, 40 µM of ABA exhibited the best results under normal and Ni-stressed conditions. ABA-mediated Ni tolerance was marked by reductions in Ni accumulation and consequent lowering of reactive oxygen species (ROS) like hydrogen peroxide and superoxide radicals. Contrarily, NO (nitric oxide) level increased in response to ABA application under Ni stress conditions, accompanied by promoted antioxidant activities through improved levels of secondary metabolites, proline, and perked-up ROS-detoxification enzymes activities. Exogenous ABA at 40 µM concentration applied to Ni-exposed plants (80 mg Ni kg-1 soil) improved the total content of alkaloids, phenolics, flavonoids and tannins by 14.3%, 10.2%, 15.4% and 7.0%, respectively, over Ni-stressed plants alone. Additionally, seed trigonelline content imparting several pharmacological actions to the fenugreek plant exhibited a remarkable escalation upto 3.6 and 2.6 mg g-1 DW under '40 µM ABA' and '40 µM ABA + 80 mg Ni kg-1 soil' treatments, respectively. The findings of the study suggest that ABA plays a key role in enhancing the overall performance of the fenugreek crop under excessive Ni stress.

Insights into strigolactone (GR24) mediated regulation of cadmium-induced changes and ROS metabolism in Artemisia annua.

Agricultural soil contamination and subsequently crops still require alternative solutions to reduce associated environmental risks. The effects of strigolactones (SLs) in alleviating cadmium (Cd) phytotoxicity in Artemisia annua plants were investigated during this study. Strigolactones play a vital role during plant growth and development due to their complex interplay during a plethora of biochemical processes. However, information on the potential of SLs to elicit abiotic stress signaling and trigger physiological modifications in plants is limited. In order to decipher the same, A. annua plants were exposed to different concentrations of Cd (20 and 40 mg kg-1), with or without the supplementation of exogenous SL (GR24, a SL analogue) at 4 µM concentration. Under Cd stress, excess Cd accumulation resulted in reduced growth, physio-biochemical traits, and artemisinin content. However, the follow-up treatment of GR24 maintained a steady state equilibrium between reactive oxygen species and antioxidant enzymes, improved chlorophyll fluorescence parameters such as Fv/Fm, ФPSII, and ETR for improved photosynthesis, enhanced chlorophyll content, maintained chloroplast ultrastructure, improved the glandular trichome (GT) attributes and artemisinin production in A. annua. Moreover, it also resulted in improved membrane stability, reduced Cd accumulation, and regulated the behaviour of stomatal apertures for better stomatal conductance under Cd stress. The results of our study suggest that GR24 could be highly effective in alleviating Cd-induced damages in A. annua. It acts via the modulation of the antioxidant enzyme system for redox homeostasis, protection of the chloroplasts and pigments for improved photosynthetic performance, and improved GT attributes for enhanced artemisinin production in A. annua.

Involvement of abscisic acid in silicon-mediated enhancement of copper stress tolerance in Artemisia annua.

Heavy metal (HM) toxicity is a well-known hazard which causes deleterious impact on the growth and development of plants. The impact of abscisic acid (ABA) in presence of silicon (Si) on plant development and quality traits has largely gone unexplored. The effects of ABA and Si on the growth, yield, and quality characteristics of Artemisia annua L. plants growing under copper (Cu) stress (20 and 40 mg kg-1) were investigated in a pot experiment. During this investigation, Cu stress caused severe damage to the plants but exogenous administration of Si and ABA ameliorated the harmful effects of Cu toxicity, and the plants displayed higher biomass and improved physio-biochemical attributes. Copper accumulated in the roots and shoots and its toxicity caused oxidative stress as demonstrated by the increased 2-thiobarbituric acid reactive substance (TBARS) content. It also resulted in the increased activity of antioxidant enzymes, however, the exogenous Si and ABA supplementation decreased the buildup of reactive oxygen species (ROS) and lipid peroxidation, alleviating the oxidative damage produced by HM stress. Copper toxicity had a considerable negative impact on glandular trichome density, ultrastructure as well as artemisinin production. However, combined Si and ABA enhanced the size and density of glandular trichomes, resulting in higher artemisinin production. Taken together, our results demonstrated that exogenous ABA and Si supplementation protect A. annua plants against Cu toxicity by improving photosynthetic characteristics, enhancing antioxidant enzyme activity, protecting leaf structure and integrity, avoiding excess Cu deposition in shoot and root tissues, and helping in enhanced artemisinin biosynthesis. Our results indicate that the combined application of Si and ABA improved the overall growth of plants and may thus be used as an effective approach for the improvement of growth and yield of A. annua in Cu-contaminated soils.

Mechanistic insight on boron-mediated toxicity in plant vis-a-vis its mitigation strategies: a review.

Boron (B) is an essential micronutrient, crucial for the growth and development of crop plants. However, the essential to a toxic range of B in the plant is exceptionally narrow, and symptoms develop with a slight change in its concentration in soil. The morphological and anatomical response, such as leaf chlorosis, stunted growth, and impairment in the xylem and phloem development occurs under B-toxicity. The transport of B in the plant occurs via transpiration stream with the involvement of B-channels and transporter in the roots. The higher accumulation of B in source and sink tissue tends to have lower photosynthetic, chlorophyll content, infertility, failure of pollen tube formation and germination, impairment of cell wall formation, and disruption of membrane systems. Excess B in the plant hinders the uptake of other micronutrients, hormone transport, and metabolite partitioning. B-mediated reactive oxygen species production leads to the synthesis of antioxidant enzymes which help to scavenge these molecules and prevent the plant from further oxidative damage. This review highlights morpho-anatomical, physiological, biochemical, and molecular responses of the plant under B toxicity and thereby might help the researchers to understand the related mechanism and design strategies to develop B tolerant cultivars.

The physio-biochemical and molecular responses and mechanism of B uptake under its toxic condition have been illustrated. The spatial distribution of boron under its toxic condition and its accumulation in the plant might be regulated with sugar alcohols (polyols). This review throws light on the elevated level of B in the soil-plant system and provides management strategies for alleviating B toxicity in the plant.

Systems biology of chromium-plant interaction: insights from omics approaches.

Plants are frequently subjected to heavy metal (HM) stress that impedes their growth and productivity. One of the most common harmful trace metals and HM discovered is chromium (Cr). Its contamination continues to increase in the environment due to industrial or anthropogenic activities. Chromium is severely toxic to plant growth and development and acts as a human carcinogen that enters the body by inhaling or taking Cr-contaminated food items. Plants uptake Cr via various transporters, such as sulfate and phosphate transporters. In nature, Cr is found in various valence states, commonly Cr (III) and Cr (VI). Cr (VI) is soil's most hazardous and pervasive form. Cr elevates reactive oxygen species (ROS) activity, impeding various physiological and metabolic pathways. Plants have evolved various complex defense mechanisms to prevent or tolerate the toxic effects of Cr. These defense mechanisms include absorbing and accumulating Cr in cell organelles such as vacuoles, immobilizing them by forming complexes with organic chelates, and extracting them by using a variety of transporters and ion channels regulated by various signaling cascades and transcription factors. Several defense-related proteins including, metallothioneins, phytochelatins, and glutathione-S-transferases aid in the sequestration of Cr. Moreover, several genes and transcriptional factors, such as WRKY and AP2/ERF TF genes, play a crucial role in defense against Cr stress. To counter HM-mediated stress stimuli, OMICS approaches, including genomics, proteomics, transcriptomics, and metallomics, have facilitated our understanding to improve Cr stress tolerance in plants. This review discusses the Cr uptake, translocation, and accumulation in plants. Furthermore, it provides a model to unravel the complexities of the Cr-plant interaction utilizing system biology and integrated OMICS approach.

Soil-applied Nickel Generates Differential Responses in Growth, Physiology and Oxidative Metabolism of Fenugreek (Trigonella foenum-graecum L.) Varieties.

This work aims to evaluate the potential of nickel (Ni), an essential micronutrient, as an oxidative stress inducer along with associated morphological and biochemical responses in different varieties of fenugreek (Trigonella foenum-graecum L.), a chief economically cultivated crop of India. Varietal differences in crop performance upon exposure to 0, 20, 40, 60 and 80 mg Ni kg- 1 soil reflects that Ni applied at 20 mg Ni kg- 1 soil offers growth-promoting effects, improved photosynthesis attributes, carbonic and nitrate reductase activities more profound in PEB followed by AFg2, AFg1 and UM185 variety. This study observed a dose-dependent reduction in all the above parameters. Maximum toxic effects were noticed at 80 mg kg- 1 Ni, manifested in the form of enhanced H2O2 and MDA contents, which were efficiently counteracted by augmentation in proline content, SOD, POX, CAT and APX activities in PEB over other varieties, suggesting that the Ni tolerance in fenugreek varieties can be organized as PEB > AFg2 > AFg1 > UM185.

Chromium in plant-soil nexus: Speciation, uptake, transport and sustainable remediation techniques.

Heavy metal (HM) pollution has become a serious global problem due to the non-biodegradable nature of the HMs and their persistence in the environment. Agricultural soil is a non-renewable resource that requires careful management so that it can fulfill the increasing demand for agricultural food production. However, different anthropogenic activities have resulted in a large-scale accumulation of HMs in soil which is detrimental to soil and plant health. Due to their ubiquity, increased bioavailability, toxicity, and non-biodegradable nature, HM contamination has formed a roadblock in the way of achieving food security, safety, and sustainability in the future. Chromium (Cr), specifically Cr(VI) is a highly bioavailable HM with no proven role in the physiology of plants. Chromium has been found to be highly toxic to plants, with its toxicity also influenced by chemical speciation, which is in turn controlled by different factors, such as soil pH, redox potential, organic matter, and microbial population. In this review, the different factors that influence Cr speciation were analyzed and the relationship between biogeochemical transformations of Cr and its bioavailability which may be beneficial for devising different Cr remediation strategies has been discussed. Also, the uptake and transport mechanism of Cr in plants, with particular reference to sulfate and phosphate transporters has been presented. The biological solutions for the remediation of Cr contaminated sites which offer safe and viable alternatives to old-style physical and chemical remediation strategies have been discussed in detail. This review provides theoretical guidance in developing suitable approaches for the better management of these remediation strategies.

Exogenous Strigolactone (GR24) Positively Regulates Growth, Photosynthesis, and Improves Glandular Trichome Attributes for Enhanced Artemisinin Production in Artemisia annua.

Artemisia annua is a medicinal plant particularly known for the production of a sesquiterpene lactone artemisinin; a specialty metabolite known for its efficacy in the treatment of malaria by killing different strains of Plasmodium falciparum due to radicals released upon the cleavage of its endoperoxide motif. Considering these facts and the immense medicinal value of artemisinin, the enhancement of in planta production of artemisinin is highly desirable. As strigolactones are known to regulate various aspects of plant growth and development, the effects of foliar spray of different concentrations of synthetic strigolactone analog GR24 (0, 0.5, 1, 2, 4, and 8 µM) on A. annua were studied. As compared to the control group, the foliar application of GR24 had a positive impact on general growth, photosynthesis, and other physiological indices with 4 µM GR24 showing the best results. The results indicate that GR24 application increased the plant biomass and various attributes related to photosynthesis, like total chlorophyll content, chlorophyll fluorescence, stomatal conductance, internal CO2, and net photosynthetic rate. Moreover, the activity of various enzymes related to photosynthesis like carbonic anhydrase, nitrate reductase, and RuBisCO was escalated. The GR24 also improved certain attributes related to glandular trichomes, with a significant enhancement in content and yield of artemisinin as compared to untreated plants.

Targeting Cd coping mechanisms for stress tolerance in Brassica napus under spiked-substrate system: from physiology to remediation perspective.

Cadmium (Cd) is a prevalent, non-essential, carcinogenic, and hazardous heavy metal that reduces plant productivity and capacity of arable land area around the globe. In the present substrate-based pot study, seedlings of Brassica napus 180015 were grown equidistantly in the spiked-substrate medium for 60 days under increasing concentrations of Cd (0, 10, 20, 30, 40, 50 mg kg-1). Following harvest, the morpho-physio-biochemical, antioxidative, and Cd-induced tolerance responses were evaluated in B. napus under an increasing Cd stress regime. Additionally, these parameters were also investigated to select the plant's threshold tolerance limit for Cd under the spiked-substrate system. B. napus showed dynamic behavior regarding morpho-physio-biochemical attributes, including agronomic features, biomass, photosynthetic pigments, relative water content under increased Cd toxicity. Cd stress-induced hydrogen peroxide (H2O2) production with high MDA contents and passive EL, followed by the orchestration of both enzymatic (SOD, POD, APX, CAT, and GR) and non-enzymatic antioxidants (flavonoids, TPC, TPA, proline, and total soluble protein) up to a certain limit. In addition, Cd-induced stress upregulated transcriptional levels of antioxidative enzyme SOD, POD, APX, GR, and MT encoded genes in B. napus. The increasing trend of Cd accumulation in different tissues at the highest Cd concentration was as follows: root > leaf > stem. In spiked substrate system, B. napus demonstrated improved metal extractability performance and a high potential for phyto-management of low to moderate Cd contamination, implying that this study could be used for integrative breeding programs and decontaminating heavy metals in real contaminated scenarios.Novelty statementThis study provides an insight into Cd-coping mechanisms of oilseed rape involved in alleviating toxicity and simultaneous phyto-management of increasing Cd concentration under spiked substrate system. The current study is the first scientific evidence of using a Cd-spiked soilless substrate medium. The present study will further strengthen our understanding of Cd-instigated positive responses in B. napus. Furthermore, it will provide a useful basis for integrative breeding programs and decontaminating heavy metals in real contaminated scenarios.

Exogenous triacontanol provides tolerance against arsenic-induced toxicity by scavenging ROS and improving morphology and physiological activities of Mentha arvensis L.

Arsenic (As), recognized as a toxic metalloid globally, has posed a serious threat to soil, plants and aquatic resources. Arsenic restrain fundamental processes of plant grown under the As-contaminated soil which are the worst sufferers for their sustenance. Thus, various scientific strategies are being continuously employed for reducing the soil As. In this regard, use of well-known plant growth regulators (PGRs) like triacontanol (TRIA) shown great efficiency towards heavy metals stress tolerance. TRIA is a plant growth promoter that has been proved effective for growth and development of plants under diverse environmental conditions for many decades. The research work was carried out to examine the toxic effects of As on various morphological, physio-biochemical, yield and quality parameters of Mentha arvensis L. and amelioration of the As toxicity through exogenous application of TRIA. Mentha plants were supplemented with various treatments (i) 0 (control), (ii) TRIA (10-6 M), (iii) As (60 mg kg-1), (iv) As (80 mg kg-1), (v) TRIA (10-6 M) + As (60 mg kg-1), and (vi) TRIA (10-6 M) + As (80 mg kg-1). Several parameters studied during the present investigation were plant height, fresh and dry weights, herbage yield, chlorophyll and carotenoid content, carbonic anhydrase (CA) activity, several chlorophyll fluorescence parameters, superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), proline (PRO), H2O2 content, TBARS content, electrolyte leakage (EL), essential oil content (EO) and yield and microscopic analyses as well as PCA analysis. Arsenic treatment exhibited deleterious effects on the overall growth, photosynthetic, and quality parameters of M. arvensis. However, the toxicity of As was mitigated by the leaf-applied TRIA to the plants, proved advantageous in combating the ROS generation.

A comprehensive review of impacts of diverse nanoparticles on growth, development and physiological adjustments in plants under changing environment.

The application of nanotechnology in agriculture includes the use of nanofertilizers, nanopesticides, and nanoherbicides that enhance plant nutrition without disturbing the soil texture and protect it against microbial infections. Thus, nanotechnology maintains the plant's health by maintaining its soil health. The use of nanoparticles (NPs) in agriculture reduces the chemical spread and nutrient loss and boosts crop yield and productivity. Effect of NPs varies with their applied concentrations, physiochemical properties, and plant species. Various NPs have an impact on the plant to increase biomass productivity, germination rate and their physiology. Also, NPs change the plant molecular mechanisms by altering gene expression. Metal and non-metal oxides of NPs (Au, Ag, ZnO, Fe2O3, TiO2, SiO2, Al2O3, Se, carbon nanotubes, quantum dots) exert an important role in plant growth and development and perform an essential role in stress amelioration. On the other hand, other effects of NPs have also been well investigated by observing their role in growth suppression and inhibition of chlorophyll and photosynthetic efficiency. In this review, we addressed a description of studies that have been made to understand the effects of various kind of NPs, their translocation and interaction with the plants. Also, the phytoremediation approaches of contaminated soil with combined use of NPs for sustainable agriculture is covered.

The Jacalin-Related Lectin HvHorcH Is Involved in the Physiological Response of Barley Roots to Salt Stress.

Salt stress tolerance of crop plants is a trait with increasing value for future food production. In an attempt to identify proteins that participate in the salt stress response of barley, we have used a cDNA library from salt-stressed seedling roots of the relatively salt-stress-tolerant cv. Morex for the transfection of a salt-stress-sensitive yeast strain (Saccharomyces cerevisiae YSH818 Δhog1 mutant). From the retrieved cDNA sequences conferring salt tolerance to the yeast mutant, eleven contained the coding sequence of a jacalin-related lectin (JRL) that shows homology to the previously identified JRL horcolin from barley coleoptiles that we therefore named the gene HvHorcH. The detection of HvHorcH protein in root extracellular fluid suggests a secretion under stress conditions. Furthermore, HvHorcH exhibited specificity towards mannose. Protein abundance of HvHorcH in roots of salt-sensitive or salt-tolerant barley cultivars were not trait-specific to salinity treatment, but protein levels increased in response to the treatment, particularly in the root tip. Expression of HvHorcH in Arabidopsis thaliana root tips increased salt tolerance. Hence, we conclude that this protein is involved in the adaptation of plants to salinity.

Crosstalk among plant growth regulators and signaling molecules during biotic and abiotic stresses: molecular responses and signaling pathways.

KEY MESSAGE: Plant stress responses are extremely sophisticated which implicate changes at the cellular, physiological and transcriptome levels by activating specific gene expression related to the challenges faced by plants.

Molecular Mechanisms of Nitric Oxide (NO) Signaling and Reactive Oxygen Species (ROS) Homeostasis during Abiotic Stresses in Plants.

Abiotic stressors, such as drought, heavy metals, and high salinity, are causing huge crop losses worldwide. These abiotic stressors are expected to become more extreme, less predictable, and more widespread in the near future. With the rapidly growing human population and changing global climate conditions, it is critical to prevent global crop losses to meet the increasing demand for food and other crop products. The reactive gaseous signaling molecule nitric oxide (NO) is involved in numerous plant developmental processes as well as plant responses to various abiotic stresses through its interactions with various molecules. Together, these interactions lead to the homeostasis of reactive oxygen species (ROS), proline and glutathione biosynthesis, post-translational modifications such as S-nitrosylation, and modulation of gene and protein expression. Exogenous application of various NO donors positively mitigates the negative effects of various abiotic stressors. In view of the multidimensional role of this signaling molecule, research over the past decade has investigated its potential in alleviating the deleterious effects of various abiotic stressors, particularly in ROS homeostasis. In this review, we highlight the recent molecular and physiological advances that provide insights into the functional role of NO in mediating various abiotic stress responses in plants.