Metagenome analyses of microbial population in geotextile fabrics used in permeable reactor barriers for toluene biodegradation.

Toluene is one of the hydrocarbons that contaminate soil and groundwater, and has a high cost to remediate, which makes it an environmental pollutant of concern. This study aimed to find bacterial distribution from nonwoven geotextile (GT) fabric specimens in a pilot-scale permeable reactive barrier (PRB). Upon 167 days of incubation with the addition of toluene, the microbial community on the GT surfaces (n = 12) was investigated by the 16S rRNA metagenome sequencing approach. According to taxonomic classification, the Proteobacteria phylum dominated the metagenomes of all the geotextile samples (80-90%). Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway database search of the toluene degradation mechanism revealed the susceptible toluene-degrading species. For the toluene-to-benzoate degradation, the Cupriavidus genus, particularly C. gilardii, C. metallidurans, and C. taiwanensis, are likely to be functional. In addition to these species, the Novosphingobium genus was abundantly localized in the GTs, in particular Novosphingobium sp. ABRDHK2. The results suggested the biodegradation potential of these species in toluene remediation. Overall, this work sheds light on the variety of microorganisms found in the geotextile fabrics used in PRBs and the species involved in the biodegradation of toluene from several sources, including soil, sediment, and groundwater. Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-023-03460-y.

Public perception towards medical waste generated in the environment during the COVID-19 pandemic in Eastern Province, Saudi Arabia.

Airborne droplets and contaminated surfaces are the main routes for the epidemic virus outbreak that causes the novel coronavirus. To reduce virus spread, people wear face masks and gloves daily, which massively increases the amount of waste generated in the environment. Also, the inappropriate disposal of used masks and gloves in communities may boost the spread of the novel coronavirus. However, no studies have been conducted to evaluate a public perception regarding the management of generated masks and gloves during the global health threat. Therefore, this paper proposes a study of public awareness, attitudes, and practices towards gloves and masks generated during the novel coronavirus pandemic in Saudi Arabia. The study showed that most of the participants (74%) have adequate awareness regarding the proper way to dispose of used masks and gloves, with 76% showing positive attitudes toward waste management. Gender shows a significant correlation with the regulations of medical waste collection and disposal (r = 0.169, p = 0.0001), and the consideration of the effective disposal of COVID-19 waste management as a collective responsibility of the community (r = 0.158, p = 0.0001). Also, the study shows that the majority of participants were aware on the availability of regulations for the collection and disposal of biomedical waste. Moreover, it demonstrates that public awareness regarding the attitude and measures taken by regional municipalities to reduce the spread of coronavirus has a significant positive correlation (r = 0.279, p = 0.0001). The study further recognized that the effective management of contagious wastes significantly protects the public against the improper practices of wastes disposal generated during the novel coronavirus pandemic.