[Effectiveness of commercial polyvalent proteinase inhibitors. Comparative analysis of their effect on kallikreins from different sources and trypsin]. / Effektivnost' deistviia kommercheskikh polivalentnykh ingibitorov proteinaz. Sravnitel'nyi analiz deistviia na kallikreiny razlichnogo proiskhozhdeniia i tripsin.

Polyvalent peptide-protein inhibitors of proteinases trasilol, contrical, hordox and pantrypine were studied using purified preparations of kallikreins from human blood plasma and hog pancreas as well as of trypsin. All the trade grade preparations of the inhibitors studied exhibited more distinct affinity to trypsin and tissue kallikrein as compared with blood plasma kallikrein. Trasilol inhibited most effectively the activity of trypsin, contrical--of pancreatic kallikrein. Contrical, trasilol and hordox inactivated blood plasma kallikrein at similar rates, pantrypine was several times less effective.

[Effectiveness of commercial polyvalent proteinase inhibitors. Use of gordox in vascular surgery]. / Effektivnost' deistviia kommercheskikh polivalentnykh ingibitorov proteinaz. Primenenie gordoksa pri khirurgicheskom vmeshatel'stve po povodu patologii sosudov.

Effect of hordox on activity of the kallikrein-prekallikrein system was studied in blood plasma of patients with stenosis of abdominal aorta during the reconstructive operation and in patients with septic complications after the open-heart operation. These impairments were accompanied by a distinct increase in kallikrein activity with simultaneous decrease in prekallikrein content, suggesting activation of the kininogenase system; the kallikrein activity was normalized in patients treated with the inhibitor. The highest efficiency of the inhibitor was observed in patients with stenosis of abdominal aorta; in patients with septic complications it was 4-fold less effective. The data obtained suggest that inhibition of the blood plasma kininogenase system correlates with restoration in these patients. The results, obtained in study of the inhibitor efficiency in vitro and in vivo, may be used for calculation of therapeutic doses of the proteinase inhibitors.

[Effect of abdominal aortic clamping on the kallikrein-kinin system and the activity of lactate dehydrogenase and its isoenzymes during reconstructive operations]. / Vliianie perezhatiia briushnoi aorty na kallikrein-kininovuiu sistemu, aktivnost' laktatdegidrogenazy i ee izofermentov pri rekonstruktivnykh operatsiiakh.

To study the effect of abdominal aorta clamping and revascularization and appraise the efficacy of the drug gordox during surgical intervention, the values of the kinin system, acid-base balance, acid metabolites, and the total activity of lactic dehydrogenase and its isoenzymatic spectrum were determined in 24 patients with aortic stenosis of atherosclerotic origin. In patients who did not receive gordox, the kinin system became markedly activated after the clamp was removed from the aorta, which led to a sharp fall in arterial pressure, the development of metabolic acidosis, and activation of enzymes of the glycolytic cycle. Preventive administration of gordox in the other group of patients during operation inhibited the processes of activation of the kinin system, stabilized hemodynamics, improved tissue circulation and prevented the development of hypoxia.

[Cardiac arrhythmias of hypothalamic origin]. / Serdechnye aritmii gipotalamicheskogo proiskhozhdeniia.

It was established in chronic rabbit experiments that periodical prolonged electric stimulation of the hypothalamic ventrolateral nucleus may induce, besides tachycardia and ventricular extrasystole, episodes of cardiac fibrillation and sudden death from ventricular fibrillation. The development of cardiac fibrillation is associated with increased content of catecholamines in the blood and myocardium. It was also found that the energy-producing activity of the mitochondria and active ion transportation were impaired in the myocardium, the intracellular content of potassium and sodium was changed, and the ultrastructure of the heart muscle was markedly altered. It was shown that the predominance of parasympathetic effects over sympathetic was one of the factors determining the resistance of the heart to the development of cardiac fibrillation of hypothalamic origin.