Assuntos
Bactérias/enzimologia , Metabolismo dos Carboidratos , Glicosídeo Hidrolases/metabolismo , Água do Mar/microbiologia , Bactérias/isolamento & purificação , Celulases/análise , Celulases/metabolismo , Galactosidases/análise , Galactosidases/metabolismo , Glucosidases/análise , Glucosidases/metabolismo , Glicosídeo Hidrolases/análise , Manosidases/análise , Manosidases/metabolismo , alfa-L-Fucosidase/análise , alfa-L-Fucosidase/metabolismoRESUMO
Nutritional attractiveness of the brown alga Laminaria japonica for the sea urchin Strongylocentrotus intermedius was studied. The composition of L. japonica was analyzed after one and two years of its life under natural conditions, in its seedlings, and in the alga partially degraded by natural factors. Substances extracted with various solvents were tested for the presence of inhibitors and activators of 1,3-beta-D-glucanase, a digestive enzyme of the sea urchin. Ethanolic extract of freshly harvested L. japonica was found to suppress the enzyme activity. Substances present in ethanolic extracts of the alga after one or two years of its life cycle and in the alga, partly degraded by natural factors, activated the sea urchin enzyme. This fact is in agreement with earlier natural observations concerning the nutritional attractiveness of such L. japonica samples for Strongylocentrotus intermedius.
