[Production of monoclonal antibodies to staphylococcal alpha-toxin using the technique of in vitro immunization]. / Poluchenie monoklonal'nykh antitel k stafilokokkovomu alfa-toksinu pri ispol'zovanii tekhniki immunizatsii in vitro.

The aim of this study is production of monoclonal antibodies (MAB) to staphylococcal alpha-toxin (SAT). SAT was obtained from culture medium of S. aureus s. 0-15 with ion-exchange chromatography and chromatofocusing. SAT was conjugated with CH-sepharose 4B and used for in vitro immunization of spleen cells, extracted from intact BALB/c mouse.