RESUMO
The present study was conducted to evaluate some of the components of antioxidant defense system and oxidative damage in 20 male patients of alcoholic liver disease (ALD). The results were compared with 20 healthy male smokers and 20 healthy male non-smokers volunteers. Patients were subjected to detailed clinical examination and laboratory investigations. Blood samples were collected for estimating reduced glutathione (GSH), total thiols (T-SH) malondialdehyde (MDA), transaminases (AST, ALT), glutathione-S-transferease (GST) and gammaglutamyl transferase (GGT). Serum aspartate amino transferase (AST)/alanine amino transferase (ALT) ratio was significantly (p<0.01) reduced in ALD patients as compared to the controls. However, the core of utility of MDA and GST was found to be significantly (p<0.01) increased in ALD patients compared to controls. There was a significant negative correlation of MDA with both GSH and TSH. Plasma GGT levels were significantly (p<0.01) increased in alcoholics and the enzyme showed a significant positive correlation with MDA. These results give enough evidence of increased oxidative stress and compromised antioxidant defense system in patients with ALD.
RESUMO
The aim of the study was to evaluate the influence of rotavirus (RV) and soyabean trypsin inhibitor (TI) on lipid composition and uptake of glucose and glycine in rotavirus (RV) infected malnourished (PEM) infant mice. Malnutrition was achieved in animals by doubling the litter size. Cholesterol (Ch) and phospholipd (PL) contents, uptake levels of glucose and glycine were determined in jejunum and ileum portion of small intestine. Increase in Ch/PL ratios was observed in PEM+RV group. The levels became comparable in PEM+RV+TI as compared to PEM. Uptake levels of glucose and glycine increased in PEM animals. With rotavirus (PEM+RV+TI group), the levels decreased which became comparable to PEM. Changes in uptake and lipid composition with rotavirus and trypsin inhibitor may be responsible for altering membrane fluidity and organization during rotavirus diarrhea. The results establish the importance of trypsin inhibitor during oral infection with rotavirus.
