No association between periodontal disease and GHQ-12in a Brazilian Police population

Objectives: We attempt to investigate a possible association between periodontal disease (PD) and mental disorders(MD) in a population of Brazilian Police.Study Method: From a total study population consisting of 803 policemen, 345 police officers were obtained bya sample calculation using the finite population correction who were randomly selected in Montes Claros, MinasGerais, Brazil. Patients who had been prescribed steroids or those diagnosed with diabetes and cardiovasculardiseases were excluded from this study. The General Health Questionnaire (GHQ-12) was used to assess mentaldisorders. Odds ratios (ORs) for periodontal diseases severity and their respective 95% confidence intervals (CIs)were estimated. The risk of advanced scores in Clinical Attachment Level (CAL) and Community PeriodontalIndex (CPI) were estimated using Poisson Regression analyses.Results: Only smoking and age were associated with severity in CAL and CPI index. No relation between MD andPD was observed even in different positions within the police department.Conclusions: It was not observed relation between GHQ-12 and the incidence of Periodontal Disease in a BrazilianPolice population. Classical factors like age and smoking, however, were associated with CAL and CPI indexhigher scores in this population (AU)

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No association between periodontal disease and GHQ-12 in a Brazilian Police population.

OBJECTIVES: We attempt to investigate a possible association between periodontal disease (PD) and mental disorders (MD) in a population of Brazilian Police. STUDY METHOD: From a total study population consisting of 803 policemen, 345 police officers were obtained by a sample calculation using the finite population correction who were randomly selected in Montes Claros, Minas Gerais, Brazil. Patients who had been prescribed steroids or those diagnosed with diabetes and cardiovascular diseases were excluded from this study. The General Health Questionnaire (GHQ-12) was used to assess mental disorders. Odds ratios (ORs) for periodontal diseases severity and their respective 95% confidence intervals (CIs) were estimated. The risk of advanced scores in Clinical Attachment Level (CAL) and Community Periodontal Index (CPI) were estimated using Poisson Regression analyses. RESULTS: Only smoking and age were associated with severity in CAL and CPI index. No relation between MD and PD was observed even in different positions within the police department. CONCLUSIONS: It was not observed relation between GHQ-12 and the incidence of Periodontal Disease in a Brazilian Police population. Classical factors like age and smoking, however, were associated with CAL and CPI index higher scores in this population.

High-throughput generation of P. falciparum functional molecules by recombinational cloning.

Large-scale functional genomics studies for malaria vaccine and drug development will depend on the generation of molecular tools to study protein expression. We examined the feasibility of a high-throughput cloning approach using the Gateway system to create a large set of expression clones encoding Plasmodium falciparum single-exon genes. Master clones and their ORFs were transferred en masse to multiple expression vectors. Target genes (n = 303) were selected using specific sets of criteria, including stage expression and secondary structure. Upon screening four colonies per capture reaction, we achieved 84% cloning efficiency. The genes were subcloned in parallel into three expression vectors: a DNA vaccine vector and two protein expression vectors. These transfers yielded a 100% success rate without any observed recombination based on single colony screening. The functional expression of 95 genes was evaluated in mice with DNA vaccine constructs to generate antibody against various stages of the parasite. From these, 19 induced antibody titers against the erythrocytic stages and three against sporozoite stages. We have overcome the potential limitation of producing large P. falciparum clone sets in multiple expression vectors. This approach represents a powerful technique for the production of molecular reagents for genome-wide functional analysis of the P. falciparum genome and will provide for a resource for the malaria resource community distributed through public repositories.