RESUMO
To date, there have been no molecular typing studies to identify the Sporothrix species circulating in Abancay, a hyperendemic area of sporotrichosis in Peru. To identify six clinical isolates of the Sporothrix schenckii complex from Abancay, Peru, we used PCR-sequencing of the calmodulin gene, and a phylogenetic analysis was conducted with these and additional sequences from GenBank. All clinical isolates were identified as S. schenckii (sensu stricto). Phylogenetic analysis revealed that the six clinical isolates from Abancay, Peru clustered in a clade along with sequences from Costa Rica, Iran, South Africa, and four other sequences from Peru. These findings reveal the presence of S. schenckii (sensu stricto) in Abancay, Peru.
Assuntos
Sporothrix , Humanos , Irã (Geográfico) , Peru/epidemiologia , Filogenia , África do Sul , Sporothrix/genéticaRESUMO
Pintomyia (Pifanomyia) deorsa (Pérez, Ogusuku, Monje & Young, 1991) was described on the basis of a single male; the female is being described here from specimens collected in Ollantaytambo, Cusco, Peru. Diagnoses for the Pintomyia genus, Pifanomyia subgenus, Verrucarum series and both sexes of Pi. deorsa are presented, as well as an identification key to distinguish the females of the Verrucarum series.
Pintomyia (Pifanomyia) deorsa (Pérez, Ogusuku, Monje & Young, 1991) fue descrita en base a un solo espécimen macho; la hembra es descrita aquí a partir de especímenes colectados en Ollantaytambo, Cusco, Perú. El diagnóstico para en género Pintomyia, el subgénero Pifanomyia, la série Verrucarum y ambos sexos de Pi. deorsa son presentados, así como claves para la identificación y separación de las hembras de la serie Verrucarum.
RESUMO
Ecological determinants of sporotrichosis etiological agents remain poorly understood. For this reason, we performed explorations using local climate estimates to determine the temperature and humidity ranges of the environment where clinically relevant Sporothrix species occur and to identify what plant species are associated with them, using data collected from the published literature. We performed a literature search to identify all publications on environmental isolations of medically relevant species of Sporothrix in the PubMed, SCOPUS, and EMBASE databases. All those studies were included in the analysis where medically relevant species of Sporothrix have been isolated from soil samples, and described a specific geographical location that could be precisely georeferenced. We approximated temperature and humidity from local climate estimates, integrating geospatial data, temperature, and water vapor pressure from regions or provinces where medically relevant species of Sporothrix have been isolated from soil. Sporothrix spp. were more commonly isolated from soil of different regions or provinces of 16 countries. Most environmental isolates were identified as S. schenckii, whereas S. pallida, S. brasiliensis, S. globosa, and S. mexicana were rare. We estimate that medically relevant Sporothrix spp. grow in the soil at temperatures of 6.6 °C to 28.84 °C and 37.5% to 99.06% relative humidity. These findings indicate that sporotrichosis etiological agents grow in soil in ecological niches from soil with wide ranges of temperature and humidity, but they are also associated with a variety of plants, flowers, woody debris, reed leaves, corn stalks, leaves, and wood crumbs, potentially facilitating its establishment and proliferation in the environment.
