RESUMO
The PowerPlex Y system including 11 Y-STRs (DYS19, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS385, DYS437, DYS438 and DYS439) was analyzed by capillary electrophoresis in 357 males from Mexico City. Haplotype frequency for this system was reported. The haplotype diversity was 99.56+/-0.04%, and gene diversity ranged from 51.4% for DYS393 to 92.5% for DYS385. AMOVA tests including previous reports from Mexico (Chihuahua and Jalisco States), demonstrated significant genetic heterogeneity between north and western populations regarding Mexico City, justifying the establishment of local databases in this country for male-identification purposes.
Assuntos
Cromossomos Humanos Y , Genética Populacional , Haplótipos , Indígenas Norte-Americanos , População Urbana , Impressões Digitais de DNA/métodos , Eletroforese Capilar , Heterogeneidade Genética , Genótipo , Humanos , Masculino , México , Controle de Qualidade , Sequências de Repetição em TandemRESUMO
We analyzed 242 individuals from the Valley of Mexico, including the larger and more cosmopolitan city of this country. They were PCR-typed for 15 STR loci with the AmpFlSTR Identifiler PCR Amplification Kit (Applied Biosystems). Allele frequencies for each STR were estimated and compared to previous reports. Genotype distribution by locus and by two-loci combination was in agreement with Hardy-Weinberg expectations for all fifteen STRs. This STR system in Mexican-mestizos presented a combined probability of exclusion (PE) and discrimination (PD) longer than 99.999%, respectively.
