Development of isoniazid electrochemical sensor using nickel ferrite - nitrogen and sulfur co-doped graphene quantum dot nanocomposite as a new electrode modifier.

This work reports the synthesis of nickel ferrite decorated nitrogen and sulfur co-doped graphene quantum dot (NF@N, S:GQD) and its use as an electrode modifier. The developed NF@N, S:GQD modified glassy carbon electrode (NF@N, S:GQD/GCE) was applied to assess isoniazid (INZ) concentration based on its oxidation at the surface of the proposed electrode. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were used as appropriate electrochemical techniques to study the electrochemical behavior of INZ and determine it. Based on combined evidence from surveys, research, and personal results, it is thought that the combination of nickel ferrite and doped graphene quantum dots can synergistically affect results, leading to increased sensitivity and reduced detection limits. This is probably mainly due to the high electrical conductivity of N, S-GQD structure, the electrocatalytic effect of nickel ferrite, and increased surface area resulting from the nano size of the modifier. The optimum conditions for preparing of the modified electrode and determination of INZ are selected by performing electrochemical experiments. The voltammetric response of the sensor is linear from 0.3 to 40 nM INZ under optimal conditions and the detection limit of the sensor is 0.1 nM. The validity and performance of the prepared sensor were confirmed by determining the amount of INZ in the drug and urine as real samples. The composite of doped nanoparticles and nickel ferrite is an innovative modification material to create electrochemical sensors with high sensitivity and selectivity that can be used in pharmaceutical applications.

An electrochemical sensor for the detection of arsenic using nanocomposite-modified electrode.

The aim of this research is to develop an electrochemical sensor based on a conducting polymer, polyaniline, and a cationic polymer, poly(diallyldimethylammonium chloride), reinforced with graphene oxide nanosheets functionalized with acrylic acid. The two-dimensional nature of acrylic acid functionalized graphene oxide nanosheets and clusters made of conductive polymers and acrylic acid functionalized graphene oxide nanosheets were confirmed by microscopic tests. The prepared nanocomposite was deposited on the glassy carbon electrode in order to prepare an electrochemical sensor for the detection of arsenic by cyclic voltammetry and differential pulse voltammetry methods. It should be mentioned that the presence of acrylic acid functionalized graphene oxide nanosheets increases the surface area due to the nano size effect and better dispersion of this nanomaterial, poly(diallyldimethylammonium chloride), increases the adsorption capacity of the analyte due to electrostatic interaction between the negatively charged analyte and positively charged surface, and polyanilin increases the charge transfer rate due to the good conductivity. The results show that the prepared electrode has a sensitivity equal to 1.79 A/M with 0.12 µM as the detection limit. The proposed sensor could be used for the determination of total inorganic arsenic by first oxidative pretreatment for conversion of As(III) to As(V).

An optical and electrochemical sensor based on L-arginine functionalized reduced graphene oxide.

The electrochemical and photochemical properties of graphene derivatives could be significantly improved by modifications in the chemical structure. Herein, reduced graphene oxide (RGO) was functionalized with L-arginine (L-Arg) by an amidation reaction between the support and amino acid. Deposition of a powerful ligand, L-Arg, on the optically active support generated an effective optical chemosensor for the determination of Cd(II), Co(II), Pb(II), and Cu(II). In addition, L-Arg-RGO was used as an electrode modifier to fabricate L-Arg-RGO modified glassy-carbon electrode (L-Arg-RGO/GCE) to be employed in the selective detection of Pb(II) ions by differential pulse anodic stripping voltammetry (DP-ASV). L-Arg-RGO/GCE afforded better results than the bare GCE, RGO/GCE, and L-Arg functionalized graphene quantum dot modified GCE. The nanostructure of RGO, modification by L-Arg, and homogeneous immobilization of resultant nanoparticles at the electrode surface are the reasons for outstanding results. The proposed electrochemical sensor has a wide linear range with a limit of detection equal to 0.06 nM, leading to the easy detection of Pb(II) in the presence of other cations. This research highlighted that RGO as a promising support of optical, and electrochemical sensors could be used in the selective, and sensitive determination of transition metals depends on the nature of the modifier. Moreover, L-Arg as an abundant amino acid deserves to perch on the support for optical, and electrochemical determination of transition metals.

An electrochemical biosensor based on a graphene oxide modified pencil graphite electrode for direct detection and discrimination of double-stranded DNA sequences.

The ability to directly recognize double-stranded DNA (ds-DNA) is a major challenge in disease diagnosis and gene therapy because DNA is naturally double-stranded. Herein, a novel electrochemical biosensor for the sequence-specific recognition of ds-DNA using a peptide nucleic acid (PNA) probe and graphene oxide (GO) modified pencil graphite electrode is reported and applied for the direct detection of the desired sequence in plasmid samples. For this purpose, GO was assembled onto the pencil graphite electrode surface (GO/PGE) by a simple casting method and applied for PNA probe immobilization (PNA-GO/PGE). Upon addition of ds-DNA, the interaction of the PNA probe with ds-DNA induces probe detachment from the electrode surface which results in a guanine oxidation signal decrease. Under optimized conditions, the guanine oxidation signal decreased linearly with the ds-DNA concentration increasing in the range from 30 pM to 10 nM, with a detection limit of 1.3 pM. Moreover, the proposed biosensor was applied for the sensitive and selective detection of double-stranded target DNA in plasmid samples. This proposed method could be used as a platform for direct detection of various sequences in double-stranded genomic DNA.

New water-soluble colorimetric pH and metal ione sensor based on graphene quantum dot modified with alizarine red S.

A new colorimetric sensor was designed for the screening pH changes in solutions, as well as, detection of some cations. The sensor preparation includes the chemical binding of alizarine red S (ARS) as a sensor of pH and cation to graphene quantum dots (GQD). Loading ARS on GQD led to the formation of water soluble sensor which finally responded to the colorimetric detection of some cations in water. Solubility and stability of the sensor in water indicate that the sensor is an ideal system for the biological and environmental applications. To demonstrate the applicability of the new sensor, the colorimetric responds of sensor were examined for some cations including Fe3+, Co2+, Ca2+, As3+, Cd2+, Hg2+, Pb2+, Sn2+, Al3+, and Cr3+. The colorimetric detections of all the ions were performable individually in a solution. In addition, GQD-ARS as a colorimetric sensor detected Co2+ at pH < 0.6 with limit of quantification 0.08 mM and Fe3+ at 0.6 < pH < 4.0 with limit of quantification 0.03 mM in the mixture of cations.

Detection and discrimination of recombinant plasmid encoding hepatitis C virus core/E1 gene based on PNA and double-stranded DNA hybridization.

Development of an electrochemical DNA biosensor for direct detection and discrimination of double-stranded plasmid (ds-Pl) without the need for denaturation of the target plasmid sample using a peptide nucleic acid (PNA) oligomer as the probe is described. This goal was achieved by modification of gold electrode with 6-mercapto-1-hexanol following monolayer self-assembly of cysteine conjugated 20-mer PNA oligomer probe, complementary to the HCV core/E1 region, which binds to ds-Pl and forms PNA/ds-Pl structure. The significant variation in differential pulse voltammetric response of methylene blue on the probe modified electrode upon contacting with complementary double-strand plasmid to form PNA/ds-Pl triplex structure is the principle of target plasmid detection. The results indicated that the reduction peak current was linear with the concentration of complementary strand in the range of 10-300 pg/µl with a detection limit of 9.5 pg/µl.