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J Fluoresc ; 29(5): 1213-1219, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31529260

RESUMO

Nitrogen doped carbon quantum dots (NCQDs) were synthesized via hydrothermal route. The NCQDs are thermally and optically stable with high flouresence yield. For the synthesis of NCQDs, citric acid and urea was taken as carbon and nitrogen sources, respectively. The Transmission Electron Microscopy (TEM) of these quantum dots revealed nearly spherical shape and average size of 1.5 nm, which was calculated using Image J software. The quantum dots were also well-characterized using spectroscopic techniques such as FTIR, UV-Visible absorption and fluorescence. These synthesized and characterized dots were utilized for selective detection of lactose in Milli Q water. The bioprobe provide a wide linear range varying from (10.00-77.41) µM with limit of detection 11.36 µM and sensitivity equal to (0.0065 ± 0.0002) µM-1. Graphical Abstract.


Assuntos
Corantes Fluorescentes/química , Lactose/análise , Lens (Planta)/enzimologia , Nitrogênio/química , Pontos Quânticos/química , beta-Galactosidase/metabolismo , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/metabolismo , Nitrogênio/metabolismo , Pontos Quânticos/metabolismo , Espectrometria de Fluorescência
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