Extending the vibroscape to agroecosystems: investigating the influence of abiotic factors and monitoring insect vibrational signaling.

Environmental conditions are crucial factors that influence communication systems and affect animal behavior. Research in the field of biotremology has improved our understanding of insect behavior, ecology, and evolution. However, the interactions between vibrational signaling and environmental factors are less studied, mainly because of technical issues faced in field trials. We therefore developed and tested an approach to investigate the effect of abiotic factors on insect vibrational signaling and explored its implementation as a monitoring tool for insect vibrational signals, using a vineyard as an agroecosystem model. Our results showed a significant decrease in insect signaling activity during unsuitable conditions of high temperature and wind velocity. We determined for the first time, the daily signaling pattern of the two insect pests, Scaphoideus titanus and Halyomorpha halys, in natural conditions. Biotremology techniques could be profitably used to monitor not only the presence of target pest species but also the biodiversity associated with vibrational signaling insects. In particular, the method implemented in this study could be used as a tool to compare the quality of cultivated areas under different management systems.

Intrasexual Vibrational Behavior of Philaenus spumarius in Semi-Field Conditions.

Insects that communicate by vibrational signals live in a complex interactive network of communication. Most studies on insect intrasexual behavior, based on plant-borne vibrational signals, have targeted few individuals. Despite their importance, behaviors that occur within groups were often overlooked. The study of multiple individuals, when insects occur in high density could simulate the environment in which they live and provide more reliable information on their behavior. In semi-field conditions, we investigated the intrasexual behavior of the meadow spittlebug, Philaenus spumarius. Vibrational signals exchanged among individuals of the same sex were recorded throughout their adult stage, from late spring to early autumn, and during the day, from the morning to the evening using a laser vibrometer. Males were less active than females throughout the season and their interactions were less frequent compared to females. Intrasexual interactions were characterized by signal overlapping in both unisex groups, in addition to signal alternating only in the case of males. In conclusion, the study of signaling behavior in intrasexual groups contributed to a better understanding of P. spumarius social behavior. We discuss the hypothesis of a possible competitive behavior between males and cooperative behavior between females.