[Serological classification of Pseudomonas aeruginosa]. / Serologicheskaia klassifikatsiia Pseudomonas aeruginosa.

In this work the comparative data on the antigenic schemes of P. aeruginosa proposed by different authors are presented. The scheme of serological grouping, accepted in the USSR, has been brought into accord with the current nomenclature of O-antigens, proposed by Lányi and Bergan. The O-antigen composition of the typing strains belonging to groups 3 and 9 according to the classification of Lányi-Bergan has been deciphered, which allows one to extend the antigenic scheme of P. aeruginosa by introducing new antigenic variants.

[Antibiotic behavior of Ps. aeruginosa strains isolated from various types of clinical material]. / Otnoshenie k antibiotikam shtammov Ps. aeruginosa, vydelennykh iz razlichnykh vidov klinicheskogo materiala.

Altogether 158 strains of P. aeruginosa isolated from the material of open and close cavities and blood of patients with bronchopulmonary diseases were studied. It was found that the majority of the virulent (92.6 per cent) and avirulent (70.3 per cent) cultures were characterized by multiple resistance to tetracycline, streptomycin, monomycin, kanamycin, levomycetin and polymyxin B with preserved sensitivity to gentamicin and carbenicillin. The source of the P. aeruginosa isolation had a significant influence on its relation to the effect of antibiotics: the highest number of the resistant cultures was isolated from the blood. No differences in the antibiotic effect on P. aeruginosa depending on the serotype were observed. No differences in the antibiotic effect on P. aeruginosa depending on the serotype were observed. Virulent cultures of P. aeruginosa typed with group agglutinating sera and isolated from the material of the open and close cavities were characterized by a higher resistance level to tetracycline, kanamycin, monomycin and levomycetin than the virulent not-typed strains.

[Principle of constructing polyvalent Pseudomonas aeruginosa vaccines]. / Printsip konstruirovaniia polivalentnykh vaktsin Pseudomonas aeruginosa.

Dependence of the range of protective action of P. aeruginosa vaccine on the number of its composites was studied. A principle of the selection of strains who vaccines differed in vivo by immunological specificity was applied to construction of the experimental preparations and modelling a polyvalent vaccine. Increase of the number of components in the vaccine was accompanied by increase of its protective action range. However, with the increase of the number of polyvaccine components in the polyvaccine the accretion of the protective effect expressed in the mean protective index per component displayed a gradual reduction. It was calculated theoretically that a 6--7-component vaccine should provide protection from 94--96% of the P. aeruginosa strains; as to further increase of the number of components--it would induce overloading of the vaccine with a possible absence of any effect.

[Serotype characteristics of clinical strains of Pseudomonas aeruginosa]. / Kharakteristika serotipov klinicheskikh shtammov Pseudomonas aeruginosa.

A total of 348 P. aeroginosa strains isolated from patients with pulmonary and pleural diseases were studied, and 87% of the test showed the possibility of their serotyping with the use of group-specific agglutinating antisera. Serogroups II, III, IV were found to be prevalent among the strains isolated from patients with bronchopulmonary pathological states. Correlations between definite groups of P. aeroginosa in their sensitivity to antibiotics were established; thus, the cultures belonging the serogroups II, III, IV were found to be more sensitive to tetracycline annd chloramphenicol than the culture belonging to other serogroups.

[Experience with epidemiologic studies of pyocyaneus infections. I. Feasibility of using serotypes and antibiotic resistance as a epidemiologic label for clinical strains]. / Opyt épidemiologicheskogo izucheniia sinegnoinoi infektsii. Soobshchenie I. Vozmozhnost' ispol'zovaniia serotipov i antibiotikorezistentnosti v kachestve épidemiologicheskoi metki klinicheskikh shtammov.

The authors carried out serological typing of 98 Pseudomonas aeruginosa strains, isolated from patients of burn department of the Sklifosovsky First Aid Institute in January-July, 1974, and of 215 strains obtained from other sources; their sensitivity to 13 antibiotics was determined. Pseudomonas aeruginosa cultures isolated from the patients were typed with O-sera of 10 serological types. The presence of several hospital strains of Pseudomonas aeruginosa was found by means of serological typing; along with these there were revealed cultures of this causative agent sporadically appearing in the department. Sensitivity to some antibiotics could serve as an additional criterion for differentiation of Pseudomonas aeruginosa strains of the same serological type.

[Dry medium for determining the beta-galactosidase activity of enterobacteria]. / Sukhaia sreda dlia opredeleniia beta-galaktozidaznoi aktivnosti énterobakterii.

Differential diagnosis of enterobacteria is based on the determination of beta-galactosidase enzyme, hydrolyzing lactose in the nutrient substrates; however, the tests suggested for its determination are time consuming and their use in practice is limited. Dry nutrient medium prepared by the authors containing o-nitrophenyl-betaD-pyranoside was tested on 1625 strains of enterobacteria in comparison with the Le Minor's ONPS test used at present. The results of beta-galactosidase determination by the, mentioned methods proved to coincide (significance level greater than 95%); this permits to recommend the method described--in dry nutrient medium--as a differential-diagnostic test for the identification of enterobacteria.

[Pyocyaneus infection of burn wounds]. / Sinegnoinaia infektsiia ozhogovykh ran

Materials of clinico-bacteriological study of 302 patients with thermal burns of different severity pointed to a considerable elevation of the incidence of Ps. aeruginosa isolation (in cultures) from burn wounds; the last few years it was found in about 50% of the cases. The greater frequency of Ps. aeruginosa detection correlated with the increase of the severity of the affection. By using Soviet set of 17 type agglutinating sera it was possible to type almost 90% of the Ps. aeruginosa cultures isolated from the wounds; a marked prevalence (over 70% of the strains) of cultures belonging to the serological group II was revealed. Patients admitted to the burn centre at early periods after the trauma displayed infection of the wound with the Ps. aeruginosa strain of the II serological group.

[Identification and serological typing of Pseudomonas aeruginosa by the indirect immunofluorescence method]. / Indikatsiia i cerotipirovanie Pseudomonas aeruginosa metodom nepriamoi immunofluorestsntsii

The authors used the method of indirect immunofluorescence for detection and serological typing of Ps. aeruginosa in pathological material. This method was found to be useful for detection of Ps. aeruginosa in association with other microorganisms. The indirect immunofluorescence method can be used for determination of the serological group of the clinical Ps. aeruginosa strains.

[An experimental study of the immunogenicity of Pseudomonas aeruginosa vaccines]. / Eksperimental'noe izuchenie immunogennosti vaktsin Pseudomonas aeruginosa

The author studied comparatively the immunogenicity of the vaccines prepared of 38 virulent Ps. aeruginosa strains belonging to different serological types. It was demonstrated that the immunogenicity of killed vaccines varied within a wide range--from the absoulte protective effect to its complete absence. The culture medium on which the initial culture was grown and the method of its detoxication produced practically no influence on the immunogenicity of the vaccines. Immunogenicity of Ps. aeruginosa vaccines apparently had no relationship with the serological type of the strains.

[Principle of obtaining specific sera for the serotyping of Pseudomonas aeruginosa]. / Printsip polucheniia spetsificheskikh syvorotok dlia serotipirovaniia Pseudomonas aeruginosa

The authors elaborated a method of obtaining sorbed specific sera for serotyping of Ps. aeruginosa on slide. A set of specific sera consisting of 20 sera was prepared; 93% of 500 cultures isolated from pathological material were typed with the aid of this set.

[Extraction and characteristics of agglutinating sera to the O-antigens of Pseudomonas aeruginosa]. / Poluchenie i kharakteristika aggliutiniruishchikh syvorotok k O-antigenam Pseudomonas aeruginosa

A method of preparation of antigens of Verder and Evans and a scheme of rabbit immunization suggested by them was used to obtain agglutinating specific O-sera to 12 type Ps. aeruginosa strains of Bulgarian and London collections. There was shown a principal correlation of the O-types of Ps. aeruginosa cultures of both collections. A possibility of using the sera obtained for typing the Ps. aeruginosa on glass was confirmed. Of 80 museum cultures 52 strains were typed; among them type 0-6 was frequently encountered.

[Hemolytic and lethal properties of Ps. aeruginosa exotoxin]. / Gemoliticheskie i letal'nye svoistva ékzotoksina PS. aeruginosa

Hemolytic and lethal properties of tbe preparations of exotoxin obtained from three Ps. aeruginosa strains in cultivation of bacteria on a hard medium covered with cellophane were studied. The exotoxin possessed a high hemolytic activity and after intraperitoneal administration caused the death of mice in the course of 24 hours. A marked thermostability and a wide range of temperature conditions and of the pH of the medium for expression of the hemolytic activity of the exotoxin was noted. Four fractions (one of which--the protein one, rapidly moving in the direction of the anode possessed hemolytic activity) were revealed by paper electrophoresis. Lethal activity failed to correlate with the hemolysin titre. At the same time the lethal factor, as in the case of hemolysin, was precipitated by trichloracetic acid and practically failed to be precipitated by chloroform; thermal treatment (70 degrees C for 30 min and 100 degrees C for 60 min) of the exotoxin failed to eliminate its toxicity; however, it somewhat delayed the periods of death of the animals.