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Methods Mol Biol ; 2793: 55-64, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38526723

RESUMO

Phage-assisted evolution has emerged as a powerful technique for improving a protein's function by using mutagenesis and selective pressure. However, mutations typically occur throughout the host's genome and are not limited to the gene-of-interest (GOI): these undesirable genomic mutations can yield host cells that circumvent the system's selective pressure. Our system targets mutations specifically toward the GOI by combining T7 targeted mutagenesis and phage-assisted evolution. This system improves the structure and function of proteins by accumulating favorable mutations that can change its binding affinity, specificity, and activity.


Assuntos
Bacteriófagos , Bacteriófagos/genética , Mutação , Mutagênese , Bacteriófago T7/genética
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