Optimization of bioprocesses with Brewers' spent grain and Cellulomonas uda.

Brewers' spent grain (BSG) is a low-value by-product of the brewing process, which is produced in large quantities every year. In this study, the lignocellulosic feedstock (solid BSG) was used to optimize fermentations with Cellulomonas uda. Under aerobic conditions, maximum cellulase activities of 0.98 nkat∙mL-1, maximum xylanase activities of 5.00 nkat∙mL-1 and cell yields of 0.22 gCells∙gBSG -1 were achieved. Under anaerobic conditions, enzyme activities and cell yields were lower, but valuable liquid products (organic acids, ethanol) were produced with a yield of 0.41 gProd∙gBSG -1. The growth phase of the organisms was monitored by measuring extracellular concentrations of two fluorophores pyridoxin (aerobic) and tryptophan (anaerobic) and by cell count. By combining reductive with anaerobic conditions, the ratio of ethanol to acetate was increased from 1.08 to 1.59 molEtOH∙molAc -1. This ratio was further improved to 9.2 molEtOH∙molAc -1 by lowering the pH from 7.4 to 5.0 without decreasing the final ethanol concentration. A fermentation in a bioreactor with 15 w% BSG instead of 5 w% BSG quadrupled the acetate concentration, whilst ethanol was removed by gas stripping. This study provides various ideas for optimizing and monitoring fermentations with solid substrates, which can support feasibility and incorporation into holistic biorefining approaches in the future.

Brewers' spent grain as carbon source for itaconate production with engineered Ustilago maydis.

Brewers' spent grain (BSG) is produced worldwide in millions of tons during the beer brewing process. Due to its high content of structural carbohydrates, BSG is a promising material for being valorised in biorefineries. In this study three process routes for producing itaconate from BSG hydrolysates are presented using the previously engineered smut fungi UstilagomaydisMB215Δcyp3ΔPria1::Petef as whole-cell biocatalyst. Using a fermentation medium based on BSG hydrolysate a yield of 0.38gIta/gSugar and a productivity of 0.11gIta/(L·h) were achieved. The addition of detoxified hydrothermal supernatant to the fermentation medium did not result in improved performance parameters but resulted in a decreased yield (0.29gIta/gSugar) and productivity (0.053 gIta/(L·h)). Simultaneous saccharification and fermentation with hydrothermal pretreated BSG is possible, although at lower rate. In summary, the valorisation of BSG in fungal fermentations might complement the end-of-life options of this industrial side product.

Brewers' spent grain liquor as a feedstock for lactate production with Lactobacillus delbrueckii subsp. lactis.

Brewers' spent grain (BSG) is a low-cost by-product of the brewing process. BSG liquor names the liquid components of BSG, mainly glucose, maltose, and long-chain α-1,4-glycosidic bond glucose oligomers. These substances should be separated in existing BSG biorefineries, as they might lead to an increased formation of microbe-inhibiting compounds in well-established hydrothermal/enzymatic saccharification processes. In most cases, this liquid fraction is discarded. The present study presents for the first time an optimized process with BSG liquor for the purpose of producing bulk chemicals (e.g., lactate) in relevant concentrations. The process comprises the application of yeast extract, produced from own brewing processes, as the sole supplemented complex constituent in a simultaneous fermentation and saccharification process. Kinetic parameters for the final optimized process conditions with the organism Lactobacillus delbrueckii subsp. lactis were: maximum specific growth rate µmax  =  0.47 h-1, maximum lactate concentration cLac, max  =  79.06 g L-1, process yield YPS  =  0.89 gLac gSugar -1, lactate production rate qP  =  4.18 gLac gCDW -1 h-1, and productivity P 15 h  =  4.93 gLac L-1 h-1. BSG liquor, linked with yeast extract from Brewers' yeast, can be a promising substrate for further bioprocess engineering tasks and contribute to a holistic and sustainable usage of Brewers' spent grain.