RESUMO
A study was made of the effect of L-DOPA on the dynamics of changes in lipid peroxidation products (LPP) and the content of various types of SH groups in certain brain structures (oblongata, cerebellum, visual and sensorimotor cortex) and their synaptosomal fractions upon irradiation. The preadministration of L-DOPA to irradiated rats inhibited LPP accumulation, prevented the decrease in the content of various types of thiols and thus exerted an antioxidant effect.
Assuntos
Encéfalo/efeitos dos fármacos , Encéfalo/efeitos da radiação , Levodopa/farmacologia , Peroxidação de Lipídeos/efeitos dos fármacos , Peroxidação de Lipídeos/efeitos da radiação , Animais , Encéfalo/metabolismo , Depressão Química , Peroxidação de Lipídeos/fisiologia , Ratos , Compostos de Sulfidrila/metabolismo , Compostos de Sulfidrila/efeitos da radiação , Sinaptossomos/efeitos dos fármacos , Sinaptossomos/metabolismo , Sinaptossomos/efeitos da radiação , Fatores de TempoRESUMO
Acute hypoxia was accompanied by intensification of lipid peroxidation in synaptosomal and mitochondrial rat brain fractions as well as by inhibition of Na+, K+- and Mg2+-ATPases. Preadministration of antioxidants vitamin E and ionol into animals prevented distinctly the increase in lipid peroxidation rate, while the ATPases activity was unaltered. Inhibition of the ATPases may be considered as a result of impairing effect of the lipid peroxidation products, which appears to be of importance for structure-functional deteriorations of the nervous system in hypoxia.
Assuntos
Adenosina Trifosfatases/metabolismo , Encéfalo/enzimologia , Hipóxia/metabolismo , Peroxidação de Lipídeos , Mitocôndrias/enzimologia , Sinaptossomos/enzimologia , Animais , ATPase de Ca(2+) e Mg(2+)/metabolismo , Hipóxia/enzimologia , Ratos , Ratos Endogâmicos , ATPase Trocadora de Sódio-Potássio/metabolismoRESUMO
The paper studies intensification of lipid peroxide oxidation in separate brain structures (the medulla oblongata, cerebellum, visual and sensomotor cortex), synaptosomal and mitochondrial fractions under hypoxia. It has been established that acute hypoxia increases accumulation of lipid peroxidation (LPO) products, hydroperoxide and malonyl dialdehyde. Intensification of LPO in synaptosomes and mitochondria is more pronounced as compared to the whole structures. Preliminary treatment with antioxidants (vitamin E and ionol) considerably suppressed LPO intensity under both hypoxia and hypoxia with reoxygenation. Intensification of LPO in synaptosomes and mitochondria is suggested to be the key point in structural-functional disturbances of the nervous system under hypoxia and ischemia.